Figure 2From: ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytesEffect of ZnO NPs on cell viability in cultured primary astrocytes. (A) MTT assay. (B) LDH release. Astrocytes were treated with 4, 8, or 12 μg/ml of ZnO NPs for 6, 12, or 24 h, respectively. (C) Inhibitory effect of N-acetylcysteine (NAC) on cell viability reduction. Astrocytes were pretreated for 1 h with 0.05, 0.5, and 5 mM of NAC before 6 h co-exposure with ZnO NPs (12 μg/ml). Data are presented as mean ± SD of the three representative experiments. **p < 0.05, ***p < 0.001 vs. control. #p < 0.05 vs. ZnO NPs (12 μg/ml).Back to article page