Table 2 Experimental design matrix used to optimize of MIP nanoparticles yield
Experiment number | Name of experiment | Run order | Inclusion/Exclusion | Concentration of monomer | Irradiation time | Temperature of irradiation | Temperature of low-affinity wash | Yield |
|---|---|---|---|---|---|---|---|---|
1 | N1 | 14 | Incl | 1 | 2.5 | 10 | 10 | 3.4 |
2 | N2 | 19 | Incl | 5 | 2.5 | 10 | 10 | 0.796 |
3 | N3 | 24 | Incl | 1 | 4.5 | 10 | 10 | 0.336 |
4 | N4 | 5 | Incl | 5 | 4.5 | 10 | 10 | 0.269 |
5 | N5 | 26 | Excl | 1 | 2.5 | 30 | 10 | |
6 | N6 | 6 | Excl | 5 | 2.5 | 30 | 10 | |
7 | N7 | 9 | Excl | 1 | 4.5 | 30 | 10 | |
8 | N8 | 4 | Excl | 5 | 4.5 | 30 | 10 | |
9 | N9 | 15 | Incl | 1 | 2.5 | 10 | 30 | 1.478 |
10 | N10 | 2 | Incl | 5 | 2.5 | 10 | 30 | 0.812 |
11 | N11 | 13 | Incl | 1 | 4.5 | 10 | 30 | 0.739 |
12 | N12 | 12 | Incl | 5 | 4.5 | 10 | 30 | 0.567 |
13 | N13 | 10 | Incl | 1 | 2.5 | 30 | 30 | 0.922 |
14 | N14 | 22 | Incl | 5 | 2.5 | 30 | 30 | 0.937 |
15 | N15 | 16 | Incl | 1 | 4.5 | 30 | 30 | 0.585 |
16 | N16 | 11 | Incl | 5 | 4.5 | 30 | 30 | 0.269 |
17 | N17 | 23 | Incl | 1 | 3.5 | 20 | 20 | 0.75 |
18 | N18 | 7 | Incl | 5 | 3.5 | 20 | 20 | 0.245 |
19 | N19 | 3 | Incl | 3 | 2.5 | 20 | 20 | 1.038 |
20 | N20 | 8 | Incl | 3 | 4.5 | 20 | 20 | 0.488 |
21 | N21 | 18 | Incl | 3 | 3.5 | 10 | 20 | 0.833 |
22 | N22 | 20 | Excl | 3 | 3.5 | 30 | 20 | |
23 | N23 | 17 | Excl | 3 | 3.5 | 20 | 10 | |
24 | N24 | 25 | Incl | 3 | 3.5 | 20 | 30 | 1.768 |
25 | N25 | 27 | Incl | 3 | 3.5 | 20 | 20 | 0.858 |
26 | N26 | 21 | Excl | 3 | 3.5 | 20 | 20 | |
27 | N27 | 1 | Excl | 3 | 3.5 | 20 | 20 |