Figure 9
From: Topographical control of cell-cell interaction in C6 glioma by nanodot arrays
Immunostaining and enlarged images showing localized and spread of Cx43 protein expression. (a) Time-dependent immunostaining of GFAP (blue) and connexin43 (red) in C6 glioma cells grown on nanodot arrays. Enhanced expression of Cx43 occurs to 10 and 39 nm at 120 h of incubation. (b) Enlarged image showing reduced and nucleus-localized expression of Cx43 protein in C6 glioma cells grown on 100-nm nanodots. (c) Enlarged image showing extensive expression of Cx43 protein spread throughout the entire cell. Scale bar = 5 μm.