Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

Table 2 Purification table of ASNase II by DEAE-Sepharose

Steps	Volume (ml)	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Overall yield^a(%)	Purification fold
Before purification (filtrate)	80	786.4	14,604.48	18.57	100	1
After purification (DEAE-Sepharose)	187	106.7	11,896.8	111.5	81.4	6.0