Preparation and nanoencapsulation of l-asparaginase II in chitosan-tripolyphosphate nanoparticles and in vitro release study

Table 4 The characteristics of ASNase II-loaded CSNPs prepared by CS/TPP 0.4%/0.095% ( w / v ) and loaded with different amounts of lyophilized ASNase II

Lyophilized protein (mg)	Size (nm)	PDI	Zeta potential (mV)	Protein content (mg)	EE (%)	Yeild (mg)	LC (%)
1	250 ± 11	0.48	+35.5 ± 2	0.701 ± 0.011	70.1	3.02	23.3
2	262 ± 10	0.38	+30.7 ± 2	1.464 ± 0.05	73.2	4.18	35.1
3	295 ± 9	0.27	+24.1 ± 3	2.244 ± 0.105	74.8	5.5	40.8
4	340 ± 12	0.42	+21.2 ± 3	3.048 ± 0.07	76.2	6.4	47.6
5	ND	ND	ND	ND	ND	ND	ND

PDI < 5 and unimodal size distribution. ND, not determined (the physicochemical characteristic of the nanoparticles prepared from 5 mg of protein was not suitable for further study); data shown are the mean ± standard deviation.