Figure 8
From: Validation of a Janus role of methotrexate-based PEGylated chitosan nanoparticles in vitro
In vitro cell viability and intracellular delivery. (A) Cytotoxicity of the PEG-CS-NPs, (FA + PEG)-CS-NPs, (MTX + PEG)-CS-NPs, and free MTX against HeLa cells after 24 h of incubation (mean ± SD, n = 6). Statistical significance: *P < 0.05. (B) Cytotoxicity of the PEG-CS-NPs, (FA + PEG)-CS-NPs, (MTX + PEG)-CS-NPs, and free MTX at the highest MTX concentration (10 μg/mL) against HeLa cells (cancer cells) or MC 3 T3-E1 cell (normal cells) after 24 h of incubation (mean ± SD, n = 6). Statistical significance: *P < 0.05. (C) Intracellular delivery of the (MTX + PEG)-CS-NPs in HeLa cells after 4 h of incubation observed by laser scanning confocal microscopy. The late endosomes and lysosomes were stained by LysoTracker Red. (a) Green fluorescent FITC, (b) red fluorescent late endosomes/lysosomes, (c) overlay of (a) and (b).