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Figure 3 | Nanoscale Research Letters

Figure 3

From: The effect of two novel amino acid-coated magnetic nanoparticles on survival in vascular endothelial cells, bone marrow stromal cells, and macrophages

Figure 3

Effects of MNPs@Gly on HUVECs. (A) Morphological observation of HUVECs treated with MNPs@Gly for 6 h (×200), 24 h (×200), and 48 h (×100) at different concentrations. (B) Hoechst 33258 staining (top) and acridine orange staining (middle) for HUVECs treated with the nanoparticles for 24 h. Hoechst 33258 staining for HUVECs treated with the nanoparticles for 48 h (bottom). Arrows indicated the dead cells. (C) WST-8 assay determined the viability of HUVECs treated with the nanoparticles for 48 h at different concentrations (*P < 0.05, **P < 0.01, ***P < 0.001 vs normal, n = 3). (D) LDH assay detected no cell necrosis. Data were expressed as relative level of LDH compared with normal (n = 3).

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