Figure 5
From: AHAPS-functionalized silica nanoparticles do not modulate allergic contact dermatitis in mice
Quantification of T lymphocytes. Cellular infiltrates of the skin in acute contact dermatitis were mainly attributed to T lymphocytes as identified immunohistochemically by the detection of CD3 antigen and visualized with the brown chromogen diaminobenzidine. Nuclei were counterstained with hemalaun. Significant differences were observed only between the acetone solvent control (A) and oxazolone (Ox)-treated groups but not between the vehicle control (B) and AHAPS-SiO2-NP-treated groups (C). The same quantitative effects were seen for T lymphocytic infiltration into the epidermis (D) and dermis (E). Data are presented as mean ± SEM; Ox-treated groups: n = 5; solvent-treated group: n = 3; ***p < 0.001.