Figure 5From: Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2Purification of Nb3 conjugating with biotin and detection of Nb1 coupling with HRP. (A) The Nb3 conjugated with biotin was purified on a streptavidin-containing column. (B) Analysis of the Nb1 coupled with HRP by ELISA. H3N2 antigen was coated onto the plate directly. Nb1, coupled with HRP, was used as a detector. After stopping the reaction, the readings were measured at 450 nm.Back to article page