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Figure 5 | Nanoscale Research Letters

Figure 5

From: Combining magnetic nanoparticle with biotinylated nanobodies for rapid and sensitive detection of influenza H3N2

Figure 5

Purification of Nb3 conjugating with biotin and detection of Nb1 coupling with HRP. (A) The Nb3 conjugated with biotin was purified on a streptavidin-containing column. (B) Analysis of the Nb1 coupled with HRP by ELISA. H3N2 antigen was coated onto the plate directly. Nb1, coupled with HRP, was used as a detector. After stopping the reaction, the readings were measured at 450 nm.

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