Figure 2

Characterization of macrophages. Immunofluorescence staining showed that more than 90% of the Ana-1 cells expressed the macrophage markers F4/80 (red, a) and MAC-3 (red, b) and the nuclei were stained with DAPI. Flow cytometry analysis of Ana-1 cells showed that F4/80 surface antigen was positive (98.6 ± 0.4) (c), whereas the isotype control was negative (d). Scale bar measures 100 μm.