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Figure 4 | Nanoscale Research Letters

Figure 4

From: A promising gene delivery system developed from PEGylated MoS2 nanosheets for gene therapy

Figure 4

Cell uptake and in vitro siRNA transfection. (a) Confocal microscopy images of HepG2 cells after incubation with MoS2–PEG–PEI/FAM-siRNA for 4 h. The fluorescence from DAPI (blue colored) and FAM-siRNA fluorescence (green colored) showed well co-localization inside cells. (b) Western blotting results to determine PLK1 expression of HepG2 cells after various treatments indicated. β-Actin was also detected as the internal control. (c) Quantitative determination of PLK1 expression for different samples based on Western blotting data from (b). (d) The expression levels of PLK1 mRNA determined by qPCR. PLK1 mRNA levels were expressed as a relative index normalized against β-actin. Error bars were based on triplicated samples. P values were calculated by the Student’s t-test: ∗, #p <0.05 (n =3).

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