Figure 6
Time-, size-, and concentration-dependent changes in particle properties during AuNP-HLM coincubations. UV–vis extinction spectra (A), variation of UV–vis absorbance value at λmax(B), hydrodynamic diameter and Z-potential (C) and photograph of solutions (D) of 7 nm (left) and 70 nm (right) AuNPs in the presence of an equal volume of HLM for increasing protein concentrations during a coincubation period of 60 min. (A) The UV–vis spectra were acquired after mixing for 10 min, and the dots highlight the maximum adsorption of each spectrum. (B) UV–vis absorbance at λmax as a function of incubation time extracted from equilibrated spectra in (A). (C) Hydrodynamic diameter of AuNPs when mixed with 0.5 (a), 5 (b), and 50 μg mL-1 HLM (c); Z-potential of AuNPs when mixed with 0.5 (a’), 5 (b’), and 50 μg mL-1 HLM (c’). (D) A digital picture of AuNP solutions after 60 min of incubation with 500 (I), 50 (II), 5 (III), 0.5 (IV), and 0 μg mL-1 HLM (V) in the presence of 100 mM PBS.