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Anisotropic micro-cloths fabricated from DNA-stabilized carbon nanotubes: one-stop manufacturing with electrode needles

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Abstract

Among a variety of solution-based approaches to fabricate anisotropic films of aligned carbon nanotubes (CNTs), we focus on the dielectrophoretic assembly method using AC electric fields in DNA-stabilized CNT suspensions. We demonstrate that a one-stop manufacturing system using electrode needles can draw anisotropic DNA-CNT hybrid films of 10 to 100 µm in size (i.e., free-standing DNA-CNT micro-cloths) from the remaining suspension into the atmosphere while maintaining structural order. It has been found that a maximal degree of polarization (ca. 40%) can be achieved by micro-cloths fabricated from a variety of DNA-CNT mixtures. Our results suggest that the one-stop method can impart biocompatibility to the downsized CNT films and that the DNA-stabilized CNT micro-cloths directly connected to an electrode could be useful for biofuel cells in terms of electron transfer and/or enzymatic activity.

Background

Over the last decade, a variety of solution-based methods have been developed to fabricate carbon nanotube (CNT) films [1-23] due to their potential application as electronic (e.g., thin-film transistors) [1,3,4,17-19] or electrochemical devices (e.g., biofuel cells [2-4,24-28] and biosensors [24,29-32]). In biofuel cells, efficient electron transfer is required between the enzymatic active site and the electrode surface. Metallic CNTs, including DNA-wrapped CNTs [27], are ideal conducting nanowires that would facilitate such electron transfer. The difficulty, however, lies in the incorporation of CNT films into such devices, because the anisotropic CNT films of submicron to micron scales need to be manipulated for optimizing the device layout.

Solution-based approaches used to prepare CNT films mainly stem from colloidal assembly methods [1-28,33] and are the most popular and versatile techniques for fabricating composite films [5,20-23]. The main advantage of the colloidal method is that it can yield various films directly at room temperature in a manner compatible with thermal, piezoelectric, or electro-hydrodynamic jet printing patterning techniques [1-4]. However, this approach also has several disadvantages as it depends on the wettability of the substrates limiting the range of viable substrates for various applications. Additionally, the force involved in the transfer process easily disrupts the original network, causing limitations in terms of the film quality and the production efficiency. Colloidal approaches thus need to be refined to incorporate the aligned arrays of CNTs into various devices.

To this end, we used the dielectrophoretic assembly method [12-19] using AC electric fields in DNA-stabilized CNT suspensions [11,23,25-27,33-36] to produce aligned arrays of CNTs. The dielectrophoretic method provides a notable advantage over other techniques because this electronic method is able to selectively collect metallic CNTs and separate them from semiconducting CNTs [15,16]. Earlier studies have demonstrated that AC fields applied via on-chip electrodes precisely control the orientation of CNT arrays [12-19]. However, the on-chip assembly systems developed so far have used electrode configurations fixed to the substrate, making electrode removal from the CNT assembly difficult without the use of a binder [37].

In this study, we overcome the difficulties in fixed electrode configuration using plug-in electrode needles inserted into a pre-assembly suspension above the substrate. Using this method, we prepared free-standing anisotropic DNA-CNT hybrid films shaped into rectangular micro-cloths of 10 to 100 μm in size using electrode needles with 0.5- μm tip diameters. Our aim is to demonstrate that the use of plug-in electrodes solves several of the main issues found in wet fabrication methods. Furthermore, our method allows hybrid CNT film assembly to be drawn from a suspension into a gaseous atmosphere in one-stop manufacturing process while maintaining structural order.

Methods

Materials

Denatured DNA has been proven to be efficient to stabilize CNT densities lower than 1 wt.% in water [11,23,25-27,33-36]. We first prepared aqueous solutions of single-walled CNTs (purity, >95%; Cheap Tubes, Grafton, VT, USA) using salmon DNA (Wako, Richmond, VA, USA) as follows: 10 mg of raw CNT soot was suspended in 5 ml of a 0.12 wt.% density solution of salmon DNA (Wako) denatured by 10-min heating at 90° C. The DNA-CNT mixture was sonicated for 60 min at a 20-kHz frequency at 50 W in a water-ice bath using a homogenizer (Branson Sonifier 250, Branson Ultrasonics, Danbury, CT, USA). The sonicated suspension was centrifuged at 650g for 10 min, and the carefully decanted supernatant was used for the dielectrophoretic assembly. The CNT concentration in the supernatant was estimated from measuring its dry weight. We further added DNA solutions at various densities to the supernatants of the DNA-stabilized CNTs.

In performing the dielectrophoretic assembly experiments, we used diluted DNA-CNT mixtures where the CNT density, C CNT, was adjusted at C CNT=0.1 wt.%. The concentrations of added DNA, C DNA, adopted in the assembly ranged from 0.06 to 0.2 wt.%. Accordingly, we investigated the effect of density ratio, α=C CNT/C DNA, in the range of 0.6≤α≤2 while maintaining the CNT density at C CNT=0.1 wt.%. It is to be noted that the formation of a nematic phase has been reported at α=1 in more concentrated suspensions (C CNT>1 wt.%) [11,33-36].

Experimental setup

A pair of tungsten needles with a tip diameter of 0.5 μm was independently controlled by two sets of patch clamp micromanipulators (NMN-21, Narishige, Setagaya-ku, Tokyo, Japan). The electrode needles were inserted into a 10- μL aliquot of CNT suspensions mounted on an inverted optical microscope (IX71, Olympus, Shinjuku, Tokyo, Japan) and were arranged in a parallel configuration. An external sinusoidal wave electric field was applied between the electrode needles using an arbitrary waveform generator (Agilent 33220A, Agilent Technologies, Santa Clara, CA, USA) along with a current amplifier (F30PV, FLC Electronics, Partille, Sweden). We adjusted two parameters of the AC fields: applied electric field strength and wave frequency. Electric fields applied were in the range of 25 to 200 kV/m, while frequencies applied were between 1 kHz and 20 MHz. We obtained a time series of assembling images via CCD camera (Q-Imaging, Retiga Exi, Surrey, British Columbia, Canada) and performed video analysis using image analysis software (ImageJ).

Observations of the prepared CNT films were also made using a digital microscope (VC7700, Omron, Kyoto, Japan), and a field emission scanning electron microscope (FE-SEM; JSM-7300F, JEOL Ltd., Akishima, Tokyo, Japan) operated at 15 kV. We also covered the substrate glass with black polymer film. Within the black polymer film, a 70- μm pinhole was created. Through the pinhole placed on a microscope stage, the dried film was observed with its edge attached to a needle. The degree of alignment was quantified using a polarizer (Olympus), a spectrometer (USB2000+, Ocean Optics, Dunedin, FL, USA), and a halogen light source with its peak intensity at approximately 600 nm.

Results and discussion

Dispersion properties of DNA-CNT mixtures

The DNA-stabilized CNTs [11,23,25-27,33-36] disperse and stabilize at a considerable range of concentrations. Therefore, an isotropic-to-nematic phase transition has been found to occur in concentrated DNA-wrapped CNT suspensions particularly at the optimal ratio of α=1 [11,33-36]. This is similar to the transition observed in lyotropic rigid-rod polymers [38]. In our experiments, six different DNA-CNT mixtures were prepared by changing the density ratio α while maintaining C CNT=0.1 wt.%. This value is much lower than the density region (C CNT>1 wt.%) where the nematic phase had been found to emerge [11,33-36]; accordingly, optical microscopy was unable to detect the emergence of a nematic order in our dilute pre-assembly suspensions.

All of the relatively dilute dispersions appeared uniform and optical micrographs displayed few aggregates (see also Movie 1 in Additional file 1) for several days. Based on these observations, we performed CNT micro-cloth fabrications using dispersions incubated at 20° C for 24 h. Thereafter, the DNA-CNT mixtures were left at 20° C for 30 days. After this period, differences were observed. First, while long-lasting uniformity was validated for α<1, we saw an apparent density gradient at α=1. Second, deposited droplets of aggregates were found at α=2 (see Figure 1). This value α=1 agrees with the isotropic-nematic transition line previously reported for dense DNA-CNT mixtures [11,33-36]. The correspondence can be attributed to the enhancement of depletion attraction [38] between CNTs with increasing α, which would ultimately lead to sedimentation. Indeed, the osmotic compression has been observed due to added polymers inducing separation of a CNT-rich anisotropic phase in equilibrium with an isotropic one at α=1 [33,35].

Figure 1
figure1

Photographs of DNA-CNT mixtures. Uniform and sedimentary dispersions were observed after 5- and 30-day incubation periods, respectively, at C CNT=0.1 wt.% and α=2.

One-stop manufacturing of CNT micro-cloths

The one-stop manufacturing process consists of three consecutive steps: electric gathering and alignment of DNA-stabilized CNT arrays in suspensions of various DNA-CNT mixtures (step 1), solvent evaporation following rinsing of the remaining dispersions (step 2), and separation from the electrodes (step 3). We describe details of this fabrication procedure using the one-stop preparation at α=1 (see also Movies 1 and 2 in Additional file 1) as an example.

Figure 2a shows a schematic diagram of CNT gathering between a parallel pair of inserted electrode needles. The electrode pair creates an electrically focused rectangle by applying a spatially inhomogeneous AC field. We set the rectangular length and width at 100 and 300 μm, respectively (step 1). After CNTs were collected in the electric well of the rectangular box, extra CNT dispersoids surrounding the electrodes were rinsed off using deionized water. Subsequently, natural drying was allowed to proceed. During this drying process, the AC field was maintained to reduce the alignment loss due to evaporation-induced solvent flow (step 2). The electrodes were independently controlled by two micromanipulators. This facilitated not only needle insertion into suspensions while lifting them off the substrate but also the gentle separation of the dry assembly from the electrodes one by one (step 3).

Figure 2
figure2

Fabrication of CNT micro-cloths using electrode needles. (a) A schematic cofiguration of the electrode needles. These electrode needles are inserted into a suspension of DNA-CNT mixture, collecting aligned CNTs between them. (b, c) Digital microscope images of a dried CNT film with its edge attached to one of the electrode needles (b) and another sheet placed on a substrate after separation from the electrode pair (c). Scale bars: 50 μm.

The suspended configuration of the electrode needles was useful to avoid the additional deposition of aggregates onto the genuine assembly during drying. Indeed, even without further rinses, the presence of extra sediments on the surface was rarely observed. Figure 2b,c displays digital microscope images of dried CNT micro-cloths fabricated at E=25 kV/m and f=20 MHz. Figure 2b shows a dried CNT micro-cloth with one side attached to an electrode, and Figure 2c presents an isolated sheet on a new cover slip. From Figure 2c, the dry film can be determined to be approximately 100 μm in width and 200 μm in length. This is somewhat smaller in length than rectangular scale established by the electrode pair. The optical micrographs in Figure 2b,c reveal that the micro-cloth edges on both images are bow-shaped due to vaporization flow, which can be clearly seen from Movies 1 and 2 in Additional file 1. This vaporization accounts for the decrease in length of the rectangular micro-cloths.

Anisotropic properties of CNT micro-cloths

We used the FE-SEM to observe aligned CNT arrays inside an assembly film placed on a Cu grid substrate in the same manner as that of Figure 2c. The left image in Figure 3 shows an FE-SEM image of the surface, whereas a superficial rent is magnified in the right micrograph of Figure 3. These FE-SEM images reveal that the CNTs were aligned in parallel to the direction of the applied electric fields. From the adjustment of the SEM focus when observing dry CNT films, we found that the mean CNT film thickness was 5 to 10 μm [39].

Figure 3
figure3

FE-SEM images of DNA-stabilized CNT arrays. The aligned CNTs constitute electrically fabricated micro-cloths. Scale bars: 2 μm.

To verify this anisotropy over a larger scale, the alignment degree was quantified using a polarized optical microscope. We performed in situ measurement of the degree of polarization (DOP) for a CNT micro-cloth with one edge to an electrode (see Figure 2b and Movies 1 and 2 in Additional file 1). The manipulability of the electrode needle allows the film position to be adjusted to overlap a pinhole, as shown in Movie 3 in Additional file 1. Upon observing the anisotropy through the pinhole, the transmitted light intensity was modulated by the rotation of a polarizing plate (see Movie 3 in Additional file 1 for an example).

We measured the optical spectra of the transmitted light at the CCD plane using the aforementioned spectrometer so that we could quantify the maximum and minimum light intensities at the wavelength of 600 nm, I max and I min, respectively, to obtain the DOP: ρ=(I maxI min)/(I max+I min). A typical set of optical spectra with a given I max and I min is shown in Figure 4a where the corresponding micrograph of the brightest micro-cloth was observed through the pinhole. From these data, the DOP of a CNT micro-cloth fabricated from DNA-CNT mixture at α=1 was found to be ρ≈42%.

Figure 4
figure4

Evaluating polarization degrees of CNT micro-cloths. (a) Optical spectra of transmitted light intensities provide I max and I min that are evaluated from red and green lines, respectively, at the wavelength of 600 nm marked by the blue line. The spectrum of red line reflects the micrograph of a manipulated CNT micro-cloth observed through a pinhole (see also Movie 3 in Additional file 1). (b) DNA concentration dependency on the DOP (ρ) at E=25 kV/m and f=20 MHz. The shaded blue line at α=1 marks that DNA-CNT mixtures form depletion-induced bundles above α=1 in correspondence with the emergence of nematic order previously reported. Error bars lie within symbols.

We then investigated the extent to which the DOP is correlated with local directional order in the pre-assembly suspension by changing α from 0.6 to 2. Figure 4b indicates the α-dependence of the mean DOP, ρ, that is obtained from averaging polarization degrees of four to six sheets of CNT micro-cloths prepared at an identical α value. It is found from Figure 4b that ρ is independent of α even though the α-range covers the isotropic-nematic transition line at α=1 for denser suspensions of C CNT>1 wt.% [29,30]. Notably, the constant DOP is ca. 40% irrespective of α=1. This is sufficiently high considering that the DOP of super aligned CNT arrays is approximately 50% unless the super-aligned arrays are further drawn to produce periodically striped films [40-42].

Electric field dependencies

In contrast to Figure 4b, Figure 5a demonstrates that the applied frequency f greatly affects the DOP when the electric field strength E and α are fixed at E=25 kV/m and α=1, respectively. In the frequency range of 1 to 20 MHz, we observed a rapid increase in ρ as f is increased, and ρ reached ca. 40% at 20 MHz. The contrasted variations in ρ for Figure 4b and Figure 5a suggest that the anisotropic feature of the CNT micro-cloths is mainly determined by the external AC field, instead of the local alignment degree prior to assembly. Figure 5b therefore shows frequency dependence in terms of the gathering rates of CNT dispersoids. Gray scale contrast was used for a time sequence of optical micrographs. As an index of the gray scale contrast, the longitudinal axis in Figure 5b indicates the normalized mean darkness G(t ) of the optical micrographs at a predetermined period of time, t =600 s, during which the AC field is applied (E=25 kV/m and f=20 MHz, as before). Here, G(t) is obtained from both spatially averaging the gray scale over the rectangular region of the CNT micro-cloth and normalizing the average to satisfy the condition G(t)=1 for black objects. Because G(t ) represents the total degree of CNT gathering, a smaller value of G(t ) is associated with a slower assembly rate.

Figure 5
figure5

Applied frequency ( f ) dependencies. We used a DNA-CNT suspension (α=1) while fixing the electric field strength at E=25 kV/m. The f dependencies of the DOP ρ (a) and the reference darkness G(t ) at t =600 s (b) are displayed. Error bars lie within symbols.

Figure 5a,b displays a similarity in the upward tendencies of both the DOP and assembly rate: ρ, as well as G(t ), increases and saturates in an overlapped frequency region. It is to be noted that previous studies using on-chip electrodes have reported a similar frequency condition (f>10 MHz) over which metallic CNTs can be selectively collected [15,16]. The combination of these results implies that the DOP is reduced as the assembly rate is diminished due to the frequency dependence of metallic dielectrophoresis [12-19]. In other words, the decrease in ρ below f=10 MHz can be ascribed either to the diminishing polarizability or dielectrophoretic behavior of the metallic CNTs. However, whether dielectrophoresis is the main cause of CNT collection between the electrode needles remains to be validated.

Hence, we further addressed the assembly mechanism based on a previous formula for the increasing number rate of gathering colloids [39]. This formula predicts that the assembly rate is proportional to E 2 when colloids undergo dielectrophoresis toward an electrically focused area. The E dependence of the assembly rate was investigated using the time evolution of G(t). In measuring G(t), the present experiments adopted 900 s as a maximum duration time because it was evaluated from weighing the drops that the open densification for 900 s yielded a maximal change in the density of the suspension of less than 10%.

Figure 6a shows four plots of G(t) at field strengths of E=25, 50, 100, and 200 kV/m and f=20 MHz. Because a maximum value for the normalized gray scale, G ref=0.95, at 900 s in the lowest electric field strength of E=25 kV/m was obtained, we used G ref, which is marked by the shaded blue line in Figure 6a, as an indicator that determines a required time t req for reaching the threshold of G ref via the equation, G(t req)=G ref. From Figure 6a, we can see that the G(t) rises faster with increasing E; correspondingly, t req is reduced by increasing E and the assembly rate can be represented by the inverse time of 1/t req. Figure 6b shows that the experimental data satisfy the proportionality of 1/t req to E 2 (the blue line in Figure 6b) consistently with the dielectrophoretic mechanism [17,37,39].

Figure 6
figure6

Assembly courses dependent on the electric field strength ( E ) in a suspension of DNA-CNT mixture ( α=1 ). (a) The normalized mean darkness G(t) of the CNT assembly area is plotted as a function of the duration time, t, to compare the time courses for E=25 kV/m (blue squares), E=50 kV/m (red circles), E=100 kV/m (green crosses), and E=200 kV/m (orange triangles) at the same frequency of f=20 MHz. (b) Log-log plot of the inverse required times, 1/t req, versus E at f=20 MHz. A light blue line, representing the proportionality of 1/t req to E 2, is shown for comparison. Standard errors fall within symbols.

Conclusions

In conclusion, we applied a spatially inhomogeneous AC field across DNA-wrapped CNT suspensions by changing the density ratio of α=C CNT/C DNA from α=0.6 to α=2; incidentally, α=1 has been found to be the isotropic-nematic transition line in concentrated suspensions of C CNT>1 wt.% [33-36]. The inhomogeneous AC fields induce dipole moments in CNTs, especially in metallic tubes, and exert a dielectrophoretic force, as well as torque, on the CNTs in relatively dilute suspensions (0.1 wt.%). This causes the anisotropic CNT-DNA hybrid films to be shaped into 100- μm-scale rectangles or micro-cloths. Shaping of the size of these micro-cloths can be accomplished by the electrode needles (the on-demand regulation of film size). Because this method using micromanipulators is capable of forming assembly films far above the substrate (the off-chip assembly) even at the air-water interface, we can avoid impurity deposition, which is particularly crucial for CNT suspension drying processes that necessarily contain sediments such as depletion-induced aggregates. Furthermore, thanks to the manipulability of individual electrodes using micromanipulators, the present system allows us to accomplish two operations (the one-stop manufacturing) that other on-chip systems have difficulty performing. First, we can prepare a dried, free-standing film with one edge attached to an electrode needle, as shown in Figure 2b. Second, the same film can be subsequently placed over a pinhole, through which a microscope spectrometer can measure the DOP (Movie 3 in Additional file 1).

Finally, we would like to emphasize that, in addition to the maximal achievable DOP value (ca. 40%) irrespective of α, CNT dispersions containing excess DNA can be a useful medium for maintaining sufficient activity of D-fructose dehydrogenase (FDH) from Gluconobacter sp., an enzyme used in biofuel cells; indeed, our colorimetric study on oxidation degrees of D-fructose due to FDH [43] shows that the enzymatic activity in pre-assembly suspension of DNA-CNT mixture at α=2 dominates that at α=1, as well as that in CNT dispersion stabilized by surfactant (Triton X-100) [44]. Our preliminary result implies that the DNA-stabilized CNT micro-cloths connected directly to an electrode could be useful for biofuel cells, as mentioned previously, due to the direct electron transfer and/or improved enzymatic activity.

References

  1. 1

    Sun DM, Liu C, Ren WC, Cheng HM. A review of carbon nanotube- and graphene-based flexible thin-film transistors. Small. 2013; 9:1188–205.

  2. 2

    Park S, Vosguerichian M, Bao Z. A review of fabrication and applications of carbon nanotube film-based flexible electronics. Nanoscale. 2013; 5:1727–52.

  3. 3

    Hu L, Hecht DS, Gruner G. Carbon nanotube thin films: fabrication, properties, and applications. Chem Rev. 2010; 110:5790–844.

  4. 4

    Cao Q, Rogers JA. Ultrathin films of single-walled carbon nanotubes for electronics and sensors: a review of fundamental and applied aspects. Adv Mater. 2009; 21:29–53.

  5. 5

    Cooper CA, Ravich D, Lips D, Mayer J, Wagner HD. Distribution and alignment of carbon nanotubes and nanofibrils in a polymer matrix. Compos Sci Technol. 2002; 62:1105–12.

  6. 6

    Zhang M, Fang S, Zakhidov AA, Lee SB, Aliev AE, Williams CD, et al. Strong, transparent, multifunctional, carbon nanotube sheets. Science. 2005; 309:1215–9.

  7. 7

    Wu Z, Chen Z, Du X, Logan JM, Sippel J, Nikolou M, et al. Transparent, conductive carbon nanotube films. Science. 2004; 305:1273–6.

  8. 8

    Lu M, Jang MW, Haugstad G, Campbell SA, Cui T. Well-aligned and suspended single-walled carbon nanotube film: directed self-assembly, patterning, and characterization. Compos Sci Technol. 2009; 94:261903.

  9. 9

    Wu J, Jiao L, Antaris A, Choi CL, Xie L, Wu Y, et al. Self-assembly of semiconducting single-walled carbon nanotubes into dense, aligned rafts. Small. 2013; 9:4142–8.

  10. 10

    Li X, Zhang L, Wang X, Shimoyama I, Sun X, Seo WS, et al. Langmuir-Blodgett assembly of densely aligned single-walled carbon nanotubes from bulk materials. J Am Chem Soc. 2007; 129:4890–1.

  11. 11

    Zamora-Ledezma C, Blanc C, Maugey M, Zakri C, Poulin P, Anglaret E. Anisotropic thin films of single-wall carbon nanotubes from aligned lyotropic nematic suspensions. Nano Lett. 2008; 8:4103–7.

  12. 12

    Monica AH, Papadakis SJ, Osiander R, Paranjape M. Wafer-level assembly of carbon nanotube networks using dielectrophoresis. Nanotechnology. 2008; 19:085303.

  13. 13

    Freer EM, Grachev O, Duan X, Martin S, Stumbo DP. High-yield self-limiting single-nanowire assembly with dielectrophoresis. Nat Nanotechnol. 2010; 5:525–30.

  14. 14

    Shekhar S, Stokes P, Khondaker SI. Ultrahigh density alignment of carbon nanotube arrays by dielectrophoresis. ACS Nan. 2011; 5:1739–46.

  15. 15

    Krupke R, Linden S, Rapp M, Hennrich F. Thin films of metallic carbon nanotubes prepared by dielectrophoresis. Adv Mater. 2006; 18:1468–70.

  16. 16

    Krupke R, Hennrich F, Lohneysen HV, Kappes MM. Separation of metallic from semiconducting single-walled carbon nanotubes. Science. 2003; 301:344–7.

  17. 17

    Islam MR, Khondaker SI. Recent progress in parallel fabrication of individual single walled carbon nanotube devices using dielectrophoresis. Mater Exp. 2014; 4:263–78.

  18. 18

    Toda T, Frusawa H, Furuta M. Thin-film transistors using uniform and well-aligned single-walled carbon nanotubes channels by dielectrophoretic assembly. Jpn J Appl Phys. 2013; 52. 03BB09.

  19. 19

    Vijayaraghavan A, Blatt S, Weissenberger D, Oron-Carl M, Hennrich F, Gerthsen D, et al. Ultra-large-scale directed assembly of single-walled carbon nanotube devices. Nano Lett. 2007; 7:1556–60.

  20. 20

    Jiang C, Tsukruk VV. Freestanding nanostructures via layer-by-layer assembly. Adv Mater. 2006; 18:829–40.

  21. 21

    Hennrich F, Lebedkin S, Malik S, Tracy J, Barczewski M, Rosner H, et al. Preparation characterization and applications of free-standing single walled carbon nanotube thin films. Phys Chem Chem Phys. 2002; 4:2273–7.

  22. 22

    Kuilla T, Bhadra S, Yao D, Kim NH, Bose S, Lee JH. Recent advances in graphene based polymer composites. Prog Polym Sci. 2010; 35:1350–75.

  23. 23

    Cooper L, Amano H, Hiraide M, Houkyou S, Jang IY, Kim YJ, et al. Freestanding bendable thin film for supercapacitors using DNA-dispersed double walled carbon nanotubes. Compos Sci Technol. 2009; 95:233104.

  24. 24

    Yang W, Ratinac KR, Ringer SP, Thordarson P, Gooding JJ, Braet F. Carbon nanomaterials in biosensors: should you use nanotubes or graphene?. Angew Chem Int Ed. 2010; 49:2114–38.

  25. 25

    Holzinger M, Le Goff A, Cosnier S. Carbon nanotube/enzyme biofuel cells. Electrochim Acta. 2012; 82:179–90.

  26. 26

    Gao F, Viry L, Maugey M, Poulin P, Mano N. Engineering hybrid nanotube wires for high-power biofuel cells. Nat Commun. 2010; 1:2.

  27. 27

    Lee JY, Shin H. Y, Kang SW, Park C, Kim SW. Improvement of electrical properties via glucose oxidase-immobilization by actively turning over glucose for an enzyme-based biofuel cell modified with DNA-wrapped single walled nanotubes. Biosens Bioelectron. 2011; 26:2685–8.

  28. 28

    Yan Y, Chan-Park MB, Zhang Q. Advances in carbon-nanotube assembly. Small. 2007; 3:24–42.

  29. 29

    Wen Z, Li J. Hierarchically structured carbon nanocomposites as electrode materials for electrochemical energy storage, conversion and biosensor systems. J Mater Chem. 2009; 19:8707–13.

  30. 30

    Lu LM, Zhang XB, Shen GL, Yu RQ. Seed-mediated synthesis of copper nanoparticles on carbon nanotubes and their application in nonenzymatic glucose biosensors. Analytica Chim Acta. 2012; 715:99–104.

  31. 31

    Zhang K, Xu J, Duan X, Lu L, Hu D, Zhang L, et al. Controllable synthesis of multi-walled carbon nanotubes/poly (3,4-ethylenedioxythiophene) core-shell nanofibers with enhanced electrocatalytic activity. Electrochim Acta. 2014; 137:518–25.

  32. 32

    Zhang K, Duan X, Zhu X, Hu D, Xu J, Lu L, et al. Nanostructured graphene oxide-MWCNTs incorporated poly (3, 4-ethylenedioxythiophene) with a high surface area for sensitive determination of diethylstilbestrol. Synthetic Met. 2014; 195:36–43.

  33. 33

    Zakri C, Blanc C, Grelet E, Zamora-Ledezma C, Puech N, Anglaret E, et al. Liquid crystals of carbon nanotubes and graphene. Philos Tr Soc A. 2013; 371:20120499.

  34. 34

    Badaire S, Zakri C, Maugey M, Derre A, Barisci JN, Wallace G, et al. Liquid crystals of DNA - stabilized carbon nanotubes. Adv Mater. 2005; 17:1673–6.

  35. 35

    Tardani F, La Mesa C, Poulin P, Maugey M. Phase behavior of DNA-based dispersions containing carbon nanotubes: effects of added polymers and ionic strength on excluded volume. J Phys Chem C. 2012; 116:9888–94.

  36. 36

    Tardani F, Gentile L, Ranieri GA, La Mesa C. Shear orientation in nematic carbon nanotube dispersions: a combined NMR investigation. J Phys Chem C. 2013; 117:8556–62.

  37. 37

    Velev OD, Bhatt KH. On-chip micromanipulation and assembly of colloidal particles by electric fields. Soft Matter. 2006; 2:738–50.

  38. 38

    Dogic Z, Fraden S. Ordered phases of filamentous viruses. Curr Opin Colloid In. 2006; 11:47–55.

  39. 39

    Frusawa H, Manabe T, Kagiyama E, Hirano K, Kameta N, Masuda M, et al. Electric moulding of dispersed lipid nanotubes into a nanofluidic device. Sci. Rep. 2013; 3:2165.

  40. 40

    Jiang K, Wang J, Li Q, Liu L, Liu C, Fan S. Superaligned carbon nanotube arrays films and yarns: a road to applications. Adv Mater. 2011; 23:1154–61.

  41. 41

    Feng C, Liu K, Wu JS, Liu L, Cheng JS, Zhang Y, et al. Flexible stretchable transparent conducting films made from superaligned carbon nanotubes. Adv Func Mater. 2010; 20:885–91.

  42. 42

    Liu K, Sun Y, Liu P, Wang J, Li Q, Fan S, et al. Periodically striped films produced from super-aligned carbon nanotube arrays. Nanotechnology. 2009; 20:335705.

  43. 43

    Ameyama M, Shinagawa E, Matsushita K, Adachi O. D-fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose. J Bacteriol. 1981; 145:814–23.

  44. 44

    Vaisman L, Wagner HD, Marom G. The role of surfactants in dispersion of carbon nanotubes. Adv Colloid Interfac. 2006; 128:37–46.

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Acknowledgments

This work was funded by the Yamada Foundation.

Author information

Correspondence to Hiroshi Frusawa.

Additional information

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

HF designed and performed the experiments, analyzed the data, and wrote the manuscript. GY contributed to obtaining the experimental results. Both authors read and approved the final manuscript.

Additional file

Additional file 1

Three movie clips of assembly (Movie 1), drying (Movie 2), and polarization (Movie 3). Movie 1: A typical sequence of preparing anisotropic DNA-CNT micro-cloth that consists of three steps: an electric gathering of aligned carbon nanotubes between a parallel pair of electrode needles, vaporization when the air-water interface moves across the assembly surface, and separation of one needle from the micro-cloth while the other electrode remains fixed to one edge of the assembly film. Movie 2: A vaporization-induced flow washing out two rectangular edges, opposite to each other, more extensively than those in Movie 1. Upon evaporation, the CNT assembly is separated from one electrode and is touched by the free needle itself, with the other electrode remaining adherent to the CNT micro-cloth. Movie 3: In the early part of this movie, the location of a dried CNT film is adjusted by manipulating an attached electrode needle so that the black sheet may cover a pinhole which can be seen as a bright circle. Subsequently, the film brightness varies according to the rotation of a polarizing plate.

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Frusawa, H., Yoshii, G. Anisotropic micro-cloths fabricated from DNA-stabilized carbon nanotubes: one-stop manufacturing with electrode needles. Nanoscale Res Lett 10, 107 (2015) doi:10.1186/s11671-015-0817-3

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Keywords

  • Carbon nanotubes
  • DNA
  • Depletion
  • Lyotropic liquid crystal
  • Free-standing film
  • Dielectrophoresis
  • Biofuel cell