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Table 2 Primary and secondary antibodies used in Western blot method

From: The effect of silver nanoparticles (AgNPs) on proliferation and apoptosis of in ovo cultured glioblastoma multiforme (GBM) cells

Western blot
Primary antibody Dilution Producer Secondary antibody Dilution Producer
Active caspase 3a 1:1,000 Cell Signaling Anti-rabbit 1:10,000 Santa Cruz
Active caspase 9a 1:1,000 Cell Signaling Anti-rabbit 1:10,000 Santa Cruz
Actina 1:200 Santa Cruz Anti-goat 1:5,000 Santa Cruz
  1. aPolyclonal antibody.