Green sonochemical synthesis of silver nanoparticles at varying concentrations of κ-carrageenan
© Elsupikhe et al. 2015
Received: 5 February 2015
Accepted: 27 April 2015
Published: 28 July 2015
A green sonochemical method was developed for preparing silver nanoparticles (Ag-NPs) in different concentrations of kappa carrageenan (κ-carrageenan). The κ-carrageenan was used as a natural eco-friendly stabilizer, and ultrasonic irradiation was used as a green reducing agent. The number of Ag-NPs increased with increasing κ-carrageenan concentrations. Formation of Ag/κ-carrageenan was determined by UV-visible spectroscopy where the surface plasmon absorption maximum was observed at 402 to 420 nm. The X-ray diffraction (XRD) analysis showed that the Ag-NPs are of a face-centered cubic structure. The Fourier transform infrared (FT-IR) spectrum indicated the presence of Ag-NPs in κ-carrageenan. Transmission electron microscopy (TEM) image for the highest concentration of κ-carrageenan showed the distribution of Ag-NPs with an average particle size near to 4.21 nm. Scan electron microscopy (SEM) images illustrated the spherical shape of the Ag-NPs. The use of photo irradiation provides a green and economic feature to this work.
In the past 10 years, researchers have paid great attention to the nanotechnology field, which deals with reaction at the atomic and molecular level. Nanotechnology contains the synthesis, characterization, and application of devices and materials whose smallest organization in at least one dimension is on a scale of less than 100 nm [1–3]. Nanotechnology offers a broad technological base for applications in several areas such as modeling, bioprocessing in industry, and molecular medicine [4–6].
Metallic nanoparticles are of interest especially in biomedical sciences and engineering because of their huge potential in nanotechnology, hence opening a wide range of potential applications in biotechnology and magnetic separations . Metallic NPs also offer applications in biomedicine and drug delivery [8, 9].
Due to its good conductivity, catalytic properties, chemical stability, and antibacterial activity, silver nanoparticles have gained much interest [10, 11]. Silver nanoparticles (Ag-NPs) have different catalytic properties like surface plasmon resonance . Ag-NPs possess strong toxicity against a wide range of microorganisms and bacterial cells and have long been used as potent bactericidal agents .
Generally, there are many methods for the synthesis of metal nanoparticles. They can be prepared by chemical and physical methods. The chemical method for the synthesis of Ag-NPs is by using a chemical reduction method [14, 15]. Physical methods for preparing metal nanoparticles as a green method can be done by using the irradiation as a reducing agent including gamma irradiation , UV-irradiation , microwave irradiation , and ultrasonic waves .
Sonochemistry is the research area in which molecules undergo a chemical reaction due to the application of powerful ultrasound radiation (20 KHz to 10 MHz) . The sonochemical method has been studied for yielding different kinds of nanomaterials, especially noble metal nanoparticles, such as gold, platinum, and lead . The sonofication mechanism is called cavitation which is the production of a radical species by generating bubbles in the solution. The bubbles grow in the solution, and when they reach maximum size, the bubbles collapse and generate high temperatures and pressure. These conditions cause breaking of chemical bonds and formation of free radicals.
Kappa carrageenan (κ-carrageenan) is found in numerous red seaweeds. This polysaccharide has a linear structure of sulfated polysaccharide of D-galactose and 3,6-anhydro-D-galactose . In the food industry, κ-carrageenan is widely used for example as a gelling agent and as a texture improvement for cottage cheese. Also, it can be used in toothpaste; air freshener gels, cosmetic creams, and shoe polish . The biological activity of carrageenan as a natural polysaccharide has carried a large increase in its use for human applications due to its chemical structure and physical properties, and κ-carrageenan has also been used in engineering for the preparation of drug vehicles for controlled release .
As κ-carrageenan is a cheap natural polymer that has a negative charge in its back bone, it can be used as a stabilizer for the synthesis of Ag-NPs. On the other hand, ultrasonic irradiation plays an important role as a reducing agent to form metal NPs with small size and high distribution. In the literature, there is no report on the synthesis of Ag-NPs by using κ-carrageenan as a stabilizer. Hence, in this work, we proposed a green synthesis method of Ag-NPs by reducing and varying the concentrations of κ-carrageenan under ultrasonic irradiation for 90 min at room temperature. For the preparation of Ag-NPs, the effect of κ-carrageenan concentration on the optical properties, structures, and morphologies of Ag-NPs were characterized by using ultraviolet-visible (UV-vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), and scan electron microscopy (SEM). This is the first report in the literature on the synthesis of nanoparticles by using different concentrations of κ-carrageenan as a stabilizer with ultrasonic irradiation as a reducing agent to form small size and highly distributed Ag-NPs.
All the reagents in this work were used as received without any purification. The κ-carrageenan was obtained from Sigma (St. Louis, MO, USA), grad type (CAS 9000-07-1), and AgNO3 was obtained from Bendosen 99.89% (C0721-2284551). The aqueous solutions were prepared by using double distilled water.
Synthesis of silver nanoparticles
The Ag-NPs were synthesized by reducing AgNO3 using ultrasonic waves in the presence of κ-carrageenan. Five suspensions were prepared, by adding 10 mL of 0.1 M AgNO3 to 40-mL κ-carrageenan. The κ-carrageenan solutions used were 0.1, 0.15, 0.20, 0.25, and 0.3 wt%, respectively. The solutions were stirred for 1 h to obtain AgNO3/κ-carrageenan. Then, the samples were exposed to high-intensity ultrasound irradiation under amplitude of 50% for 90 min at room temperature. Ultrasound irradiation was carried out with ultrasonic liquid processors (Hielscher ultrasound UP-400S, Teltow, Brandenburg, Germany, 50/100 Hz) immersed directly into the reaction solution. After that, the suspensions were centrifuged for 15 min and washed with double distilled water four times to remove the silver ion residue. The nanoparticles were precipitated then dried at 40°C under vacuum overnight to obtain the Ag-NPs.
Characterization methods and instruments
The Ag/κ-carrageenan nanoparticles were characterized using UV-vis spectroscopy, XRD, FT-IR spectroscopy, TEM, and SEM. The UV-vis spectra were recorded over a range of 300 to 800 nm with the H.UV.1650 PC, SHIMADZU UV-vis spectrophotometer (SHIMADZU, Kyoto, Japan). The XRD patterns were carried out on a Philips X’pert (Cu Kα, Philips, Amsterdam, Netherlands) and were recorded at a scan speed of 2°/min. FT-IR spectra were recorded over the range of 500 to 4,000 cm−1 with a series 100 PerkinElmer FT-IR 1650 spectrophotometer (PerkinElmer, Waltham, MA, USA). TEM observations were carried out on a Hitachi H-7100 electron microscope (Hitachi, Chiyoda, Tokyo, Japan), and the particle size distributions were determined using the UTHSCSA Image Tool version 3.00 program. SEM was carried out on Jeol-JSM-7600F (Jeol, Tokyo, Japan).
Results and discussion
UV-visible spectroscopic analysis
As shown in Figure 2a, when the concentrations of κ-carrageenan increased (0.1%, 0.15%, 0.2%, 0.25%, and 3% in a, b, c, d, and e, respectively), the intensity of the SPR peak also regularly increased. The increase of the absorbance was indicative that the concentration of Ag-NPs increased 
Furthermore, Figure 2b shows that with an increase of the concentrations of κ-carrageenan, the absorbance also increased and shifted to lower wavelength at 402 nm to blue-shift, which referred to a decrease in the particle size [31, 32]. Based on Mie’s theory , nanoparticles with different sizes should demonstrate different optical properties due to the difference in the SPR bands.
In Figure 2c it was observed that the 0.3% κ-carrageenan solution had a larger absorbance compared to other samples. The increase of the absorbance indicated that the concentration of Ag-NPs increased .
X-ray diffraction analysis
FT-IR chemical analysis
In summary, we reported the successful use of ultrasonic irradiation as a green and physical reducing method for the synthesis of Ag-NPs in different concentrations of κ-carrageenan. The Ag-NPs were successfully formed as proven by the maximum surface plasmon resonance peak at 402 to 420 nm for each sample as indicated by UV-vis spectroscopy. The XRD pattern also showed the fcc crystal structure of Ag-NPs without any impurity for all the samples. FT-IR showed the interactions that exist between κ-carrageenan and Ag-NPs. The TEM images and their particle size distributions indicated that as κ-carrageenan concentrations increased, the number of Ag-NPs also increased. SEM data showed that with increasing κ-carrageenan concentration, there were change in the surface of Ag/κ-carrageenan, where small-sized Ag-NPs with spherical shape were obtained.
The authors are also grateful to the staff of the Institute of Advanced Technology, Department of Chemistry UPM, for their help in this research, and the Institute of Bioscience (IBS/UPM), for technical assistance.
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