Fig. 5
C60–COOH prevents loss of mitochondrial membrane potential and generation of mitochondrial reactive oxygen species (ROS) induced by LPS. BV-2 cells were treated with 1 μg/mL of LPS for 6 h in the absence or presence of 50 μM C60–COOH pretreatment for 6 h. The cells were then incubated either with MitoSOX Red and JC-1 fluorescent probes, respectively, and mitochondrial ROS levels and mitochondrial membrane potential were observed under a laser confocal microscope (a and c) or analyzed by flow cytometry (b and d). Representative images are shown. *p < 0.05, significantly different from LPS-treated control