Fig. 6
a Cytoplasmic and nuclear fractions from BV-2 cells treated with 1 μg/mL of LPS for 6 h with or without 50 μM C60–COOH pretreatment for 6 h were analyzed by western blotting using antibodies against NF-κB p65. Lamin B and β-actin were used as nucleus and cytoplasmic marker, respectively. b The ratio of NF-κB p65 in nucleus and cytoplasm was performed by densitometric analysis. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05, significantly different from LPS-treated control. c Immunolocalization of NF-κB p65 was studied by confocal laser scanning microscopy