Fig. 8
a COX-2 and iNOS expression were analyzed by western blotting analysis in BV-2 cells treated with 1 μg/mL of LPS for 12 h in the absence or presence of pretreatment with 50 μM C60–COOH for 6 h. b, c The relative levels of COX-2 and iNOS expression were performed by densitometric analysis. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05, significantly different from LPS-treated control. b, d BV-2 cells were pretreated with 50 μM C60–COOH for 6 h and then stimulated with 1 μg/mL LPS for 12 h. The amount of PGE2, NO, TNF-α, IL-β, and IL-6 released into media were measured as described in the “Methods” section. The data are expressed as the mean ± SD of three independent experiments. *p < 0.05, significantly different from LPS-treated control