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Nanoscale Research Letters
Fig. 5 | Nanoscale Research Letters

Fig. 5

From: Enhanced Antifungal Activity by Ab-Modified Amphotericin B-Loaded Nanoparticles Using a pH-Responsive Block Copolymer

Fig. 5

AMB deformations induce HKC damage. Immunofluorescence, RT-PCR, and Western blot were performed to evaluate the cytotoxicity on HKC cells treated with free AMB and CDA-AMB-NPs at 10.0 μM for 48 h. a Immunofluorescence staining with anti-KIM-1 and NGAL antibody. KIM-1 was stained with red and NGAL was stained with green. b KIM-1 and NGAL mRNA levels were analyzed by RT-PCR. c, d KIM-1 and NGAL protein levels were analyzed by Western blot. Data are shown as mean ± S.E.M. *p < 0.01, compared with the free AMB group

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