Fig. 5

a Cytotoxicity of free DOX or DOX-incorporated HAssLG nanoparticles against MDA-MB231 cells. DOX or DOX-incorporated nanoparticles were exposed to cells 24h. b. Cytotoxicity of DOX-incorporated nanoparticles in the presence (+) or absence (-) of free HA against MDA-MB231 cells (*p < 0.05). MDA-MB231 cells were pretreated with free HA 1 h before drug treatment, and then cells were exposed to 50 μg/ml of DOX or nanoparticles for 4 h. Twenty-four hours later, viable cells were determined using the MTT assay