Table 1 Primer sequences for the analysed genes in J774A.1 cells
From: AMP-Conjugated Quantum Dots: Low Immunotoxicity Both In Vitro and In Vivo
Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
β-actin | TTCTTTGCAGCTCCTTCG | TCGTCGCCCGCGAAGCC |
TLR-2 | GTCGTTCAAGGAGGTGCG | TGAGGTTTCGGTAAGTTGT |
TLR-3 | GGAGCCAGAACTGTGCCAAATACTC | TCGCAACGCAAGGATTTTATTTTTT |
TLR-4 | TCTGGTGGCTGTGGAGAC | TTGTGAGCCACATTGAGT |
TLR-5 | TGGGGACCCAGTATGCTAACT | CCACAGGAAAACAGCCGAAGT |
TLR-7 | CTTACCCTTACCATCAAC | TTGGACCCCAGTAGAAC |
TLR-9 | CGGGAACTGCTACTACAAGA | GGTTGTTATACTTCAGAGAC |
MyD88 | ACTCGCAGTTTGTTGGATG | TGTAAAGGCTTCTCGGACT |
NF-κB | TTCCAGGTGACAGTGCGG | GAGTTCCGGTTTACTCGG |
I-κB | GCCCTTCCTCCCTAACTG | GGGTAGCGAACTTGAAAAC |
TNF-α | TCTCATTCCTGCTTGTGG | GAGGGTCTGGGCCATAGA |
IL-1β | TCGTGCTGTCGGACCCAT | TCCTTTGAGGCCCAAGGCCA |
TGF-β | CTC TCCACCTGCAAGACCAT | CTGCCGTACAATTCCAGTGA |
MCP-1 | TGAGGTGGTTGTGGAAAAGG | CCTGCTGTTCACAGTTGCC |