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Scalable Fabrication of Quasi-Three-Dimensional Chiral Plasmonic Oligomers Based on Stepwise Colloid Sphere Lithography Technology
Nanoscale Research Lettersvolume 10, Article number: 393 (2015)
We report a simple and scalable method for the fabrication of spiral-type chiral plasmonic oligomers based on the stepwise colloid sphere lithography technology. Through carefully adjusting the azimuthal angle Φ of polystyrene (PS) sphere array monolayer and the deposition thickness k n , the chiral plasmonic oligomers composed of four achiral particles can be successfully fabricated on a desired substrate. And their chiral sign, i.e., left-hand or right-hand, is dependent on the anticlockwise or clockwise deposition sequence of the achiral particles. The measured results show a large chiroptical resonance in the visible region, and this resonance can be easily adjusted by using different sizes of PS spheres. Our in-depth theoretical and experimental researches further reveal that the obtained chiral plasmonic oligomers are indeed a kind of quasi-three-dimensional chiral nanostructures, which own a three-dimensional geometrical morphology, but with nonreciprocity chiroptical effect. The ease and scalability (>1 cm2) of the fabrication method make chiral plasmonic oligomers promising candidates for many applications, such as chiral biosensor and catalysis.
According to Lord Kelvin, any geometrical figure or group of points is defined as chiral if its mirror image cannot be made to coincide with itself . Chirality is ubiquitous in nature and plays a crucial role in the living body. Almost all of the important biomolecules, such as proteins, amino acids, and DNA, own the same chirality, i.e., left-hand (LH) and right-hand (RH). And distinguishing the chirality of molecules becomes very significative for life. Although enantiomers (i.e., left-handed (LH) and right-handed (RH) substances) possess the same physical and chemical properties, they usually interact differently with left- and right-circularly polarized light (LCP and RCP). In fact, the cross coupling between the electric and magnetic dipoles in the chiral medium can break the degeneracy between the helicity eigenmodes of light (i.e., LCP and RCP), leading to an absorption difference (i.e., circular dichroism, CD) or a phase retardation difference (i.e., circular birefringence, CB) for the LCP and RCP. This measurable chiroptical effect paves a way to achieve the structural characterization and spectroscopy of chiral molecules and manipulate the polarization state of light. However, the magnetoelectric coupling in the natural chiral molecules, such as DNA, proteins, amino acids, and viruses, is very weak . This limits the ability of practical applications for the polarization manipulation and high-sensitivity sensing.
Recently, chiral plasmonic structures (CPS) have been demonstrated to own giant chiroptical effect  and attracted many attentions. These CPS cannot only be used to enhance the chiroptical effect of chiral molecules and improve the detection sensitivity [4, 5] but also be used to achieve some amazing physical phenomena, such as negative refraction [6, 7], repulsive Casimir force , and spin Hall effect . These remarkable properties make CPS a very popular metamaterial in many fields, such as biology, pharmacology, and nanophotonics. Typically, the currently developed CPS can be divided into two categories: one is the two-dimensional (2D) CPS. This type of CPS owns a plane-like geometrical morphology, and the twist sense of their structures is reverse when looked from the opposite side, such as a gammadion structure , L-shaped structure , and rose-shaped structure . The chiroptical effect from these CPS is nonreciprocal, which is similar to the Faraday effect . The other is the three-dimensional (3D) CPS. The twist sense of this type of CPS is the same when looked from the opposite side, such as Helix , twisted arcs [3, 15], Y-shaped , U-shaped , cross-shaped , and three-dimensional triple-helical nanowires [19, 20]. The chiroptical effect from these CPS is reciprocal, just like that from natural light active medium . However, the fabrication of these 2D or 3D CPS usually relied on the electron beam lithography technique , direct laser writing technique , and so on. The process technology usually is time-consuming, expensive, and small area limited, which limited the related research and applications.
Molecule assembly techniques, such as DNA origami technique [24, 25], have been demonstrated to a kind of efficient and low-cost method for the fabrication of CPS. The chiroptical resonance from the CPS is randomly dispersed in solvent usually located in the visible region. Glancing angle deposition technique is another low-cost, material-independent, and parallel fabrication technique for the fabrication of CPS. With the help of various masks, such random nanohole array , square nanoparticle array , and aligned ZnO nanorods , various CPS with chiroptical resonance located in the near-infrared and visible region have been achieved. Particularly, our previous work also demonstrates that with the help of assembled polystyrene (PS) sphere array, a kind of shell-like chiral nanostructures can be achieved . This kind of CPS can combine the magnetic or dielectric spheres to form chiral “Janus particles”  or “patchy particles” [31, 32] and will find potential applications in the colloid field. However, the cancellation effect originated from the PS array limited the related applications. In this paper, we propose a new method for the fabrication of CPS based on stepwise colloid sphere lithography technology, which is quasi-three-dimensional chiral plasmonic oligomers (CPO) composed of four achiral particles. Furthermore, we systematically analyze the optical properties of our fabricated CPO with the help of full-wave simulation and the measurement of CD spectra and optical activity. And the results reveal the reciprocal chiroptical effect of our fabricated CPO.
This section describes the design principle and methods of the CPO fabricated. Colloid sphere lithography technology is a powerful method for the fabrication of nanostructures. And a great number of nanostructures have been obtained by this method, such as triangle particle array , binary array , and multiplex quasi-3D grids . The basic principle of this technology is the use of a PS array as mask, and the morphology and size of the obtained nanostructures are dependent on the projection of gap between PS spheres with respect to material vapor beam . In this work, we employ this technology to arrange four achiral nanoparticles with different sizes in a concentric circle, and in this case a kind of new CPS, i.e., CPO, can be formed on substrate, as shown in Fig. 1a, b. The left-handed chiral plasmonic oligomers (LHCPO) are related to the anticlockwise deposition sequence (Fig. 1a), while the right-handed chiral plasmonic oligomers (RHCPO) are related to the clockwise deposition sequence (Fig. 1b). In order to obtain CPO with high optical chirality and geometrical chirality, the size and position of the four achiral particles should be properly adjusted in the fabrication process.
Figure 2a is the material deposition schematic diagram, where θ is the deposition angle, which is defined as the material vapor beam with respect to the substrate normal, and Φ is the azimuth angle with respect to the PS array monolayer. In the formation of CPO, the size of the achiral particles and the diameter of the circle where the achiral particles are located are dependent on the deposition angle θ, and the position is dependent on the azimuth angle Φ. The deposition thickness k is another important parameter and defined as the deposition thickness on the plane perpendicular to the vapor beam. In general, the height of the achiral particles is dependent on the deposition thickness k. However, due to surface energy minimization , the deposition thickness k will indirectly affect the size of achiral particles and result in great contraction of the achiral particles with small deposition thickness. In the fabrication of CPO, these influence factors should be considered. The inset scanning electron microscope (SEM) images shown in Fig. 2b are the obtained CPO with θ = 20°, k 1, 2, 3, 4 = 8, 25, 40, and 55 nm, and ΔΦ = ±60°, where k n is the deposition thickness in the n-th step material deposition process, ΔΦ is the relative azimuth angle between the approaching two-step material deposition processes, and the signs “+” and “−” refer to the anticlockwise deposition sequence (i.e., LHCPO) and the clockwise deposition sequence (i.e., RHCPO). The measured CD spectra show that the LHCPO and RHCPO nearly own opposite CD spectra and a maximum CD signal of ~180 mdeg is obtained at 637 nm. These experiment results indicate the effectiveness of the method proposed in this paper. Due to the predominant fabrication method, the CPO with area of >1 cm2 can be easily obtained. These features will make CPO a promising candidate for many applications, such as chiral biosensor and catalysis.
The quasi-3D CPO are fabricated on a BK7 glass substrate through four-step Ag deposition on a monolayer of PS array. The detailed fabrication schematic diagram is shown in Fig. 3.
Firstly, a BK7 glass is used as substrate. Before being used in the micro-sphere-assembled process, the BK7 glass substrate is immerged in a mixed solution of sulfuric acid/hydrogen peroxide (3:1) for more than 1 h at 80 °C. Then, the substrate is rinsed in deionized water for several times and immerged in another mixed solution of ultrapure water/ammonium hydroxide/30 % hydrogen peroxide (5:1:1) for 1 hour under ultrasonication, sequentially. Finally, the substrates were thoroughly rinsed with deionized water and stored under deionized water until required. Secondly, a monolayer of PS array with hexagonal symmetry is obtained on the BK7 glass substrate through the method reported in the literature, as shown in Fig. 3b. The PS spheres with a diameter of 750 nm and weight concentration of 10 % are purchased from Thermo Scientific Company and used without further treatment. Thirdly, the Ag deposition on the closely packed PS array monolayer is performed inside a vacuum thermal evaporation system, as shown in Fig. 3c. The base pressure, the deposition rate, and the temperature are 4 × 10−4 Pa, 1 nm/s, and 30 °C, respectively. For a single-step deposition, the deposition time needs to be precisely controlled (i.e., deposition thickness k), together with the angles θ and Φ, which can be easily adjusted by tilting and rotating the samples with the improved slide holder in the vacuum chamber, respectively. For stepwise depositions, the operational process is the repeat of the single deposition. All the deposition angle θ is set to 20°, ΔΦ is set to ±60o for LHCPO (i.e., +60°) and RHCPO (i.e., −60°), and the deposition thickness k 1, 2, 3, 4 is set to 8, 25, 40 and 55 nm, respectively. Finally, a polydimethylsiloxane (PDMS) stamp is used to remove the silver-coated PS array monolayer. As shown in Fig. 3d, e, the PDMS stamp is placed on the surface of the silver-coated PS array monolayer with a uniform pressure of about 500 g/cm2. After being lifted up, the PS array monolayer is transferred to the surface of the PDMS stamp, due to the larger Van der Waals force from the PDMS stamp when compared with the silica substrate. When all of the process is finished, a kind of quasi-3D CPO is achieved on the BK7 glass substrate.
Results and Discussion
The SEM images are collected by the Hitachi S-4800 SEM. Figure 4a, b shows the SEM images of the obtained LHCPO and RHCPO, respectively. And the insets are the enlarged SEM images of single CPO. From these images, we can get that the CPO is well formed on the BK7 glass substrate. Obviously, the chiral sign (i.e., LH or RH) is dependent on the anticlockwise or clockwise deposition sequence. The Nikon Eclipse Ti-U atomic force microscopy (AFM) also has been used to verify the 3D geometric morphology of the quasi-3D CPO, and the obtained AFM images are shown in Fig. 4c, d, where the 3D geometric morphology is well presented. The deposition thickness in every material deposition step is also detected by AFM, and the results meet well with that designed thickness.
The finite difference time domain (FDTD) method is used to achieve the theoretical simulation and analysis of the obtained quasi-3D CPO. The geometric model of a single CPO used in simulation is four achiral particles with side length L 1, 2, 3, 4 = 36, 96, 150, and 175 nm and thickness k 1, 2, 3, 4 = 8, 25, 40, and 55 nm, respectively. These achiral particles are accurately arranged in a concentric circle like that shown in the obtained SEM images (i.e., Fig. 4a, b for LHCPO and RHCPO). Figure 5a shows the calculated CD spectra, where a maximum CD signal of about 320 mdeg is found at 680 nm. This obvious CD signal indicates that the CPO interact differently with LCP and RCP. Due to the small duty ratio of CPO on the glass substrate, the transmittance coefficient for LCP and RCP is larger than 0.70 in the whole visible range. These calculated results meet well with the CD spectra measured from the Jasco J-810 circular dichroism spectroscopy system (as shown in Fig. 5b), where the maximum CD signal is ~180 mdeg at 640 nm. The differences between the simulated and measured results should be mainly attributed to the geometric morphology difference of CPO used in theoretical simulation and experiment process.
The geometric morphology plays an important role in determining the chiroptical effect. As discussed earlier, the chiroptical effect from 2D CPS usually is nonreciprocal, while the chiroptical effect from 3D CPS is reciprocal. This optical effect gives us a standard to determine the dimensionality of CPS. For the CPO obtained in this paper, it seems that they are a kind of 3D CPS from the point of view of geometric morphology. However, our in-depth theoretical and experimental researches reveal that the obtained CPO is indeed a kind of quasi-3D CPS, and their chiroptical effect is nonreciprocal, although they own a 3D geometrical morphology. Figure 6a, b shows the simulated CD spectra of LHCPO and RHCPO on the BK7 glass substrate when light incidents from the forward (blue curve) and backward (red curve) sides. The forward side is defined as the case that the BK7 glass substrate with the CPO side faces the light source, while the backward side is defined as the opposite case. These simulated results show that the CD signals from LHCPO and RHCPO own the same sign in the whole waveband from 300 nm to 800 nm, when light incidents from the forward and backward sides. These results meet well with the experiment, as shown in Fig. 6c, d. This 3D chiroptical effect indicates that the CPO together with BK7 glass substrate is a kind of 3D CPS.
However, we note that the substrate also can affect the chiroptical effect from CPS. In fact, the excited surface plasmonic resonance in the air CPS and CPS substrate interfaces can destroy the mirror symmetry of 2D CPS and results in 3D chiroptical effect . And this phenomenon happens again in this work. Figure 7e, f shows the simulated CD spectra of LHCPO and RHCPO without substrate when light incidents from the forward and backward sides. We can see that the CD signals almost own the opposite sign when light incidents from the opposite sides. This indicates that the chiroptical effect from CPO themselves is nonreciprocal, just like that from 2D CPS. And the 3D chiroptical effect shown in Fig. 6 should be attributed to the influence of the BK7 glass substrate, which adds another dimensionality for the quasi-3D CPO and results in reciprocal chiroptical effect. When comparing the simulated results in Fig. 6a, b with Fig. 7e, f, we find that the glass substrate also can suppress some plasmonic resonance modes and lead to a relatively smooth CD spectra shown in Fig. 6a, b. Figure 7a–d shows the surface electric distribution of LHCPO when LCP incidents from the backward side. Figure 7a, c is the electric distribution of LHCPO without substrate when incident lights are 650 and 705 nm, respectively. While Fig. 7b, d is the related electric distribution of LHCPO with substrate. These images clearly show that the BK7 glass substrate can effectively change the near-field distribution of LHCPO and finally the chiroptical effect. The CD intensity difference when light incidents from front and back sides (as shown in Fig. 7e, f) should be attributed to the influence of the 3D geometrical morphology of CPO, which is different when looking from the opposite sides.
The optical activity also has been investigated in this work. We note that the obtained CPO do not own a perfect in-plane rotational symmetry, and the polarization rotation of linear polarized light does not unambiguously determine the optical activity in the obtained CPO sample. Thus, the polarization analysis for linearly polarized incidence at a series of incident polarization azimuthal angles φ is performed to specify the linear birefringence and obtain the actual optical activity. The measured diagram is shown in Fig. 8a, where a linear polarization light at 632.8 nm incidents normally to the sample surface, and the polarization rotation angle Δ of the transmitted light is defined as the longer axis of the polarization ellipse with respect to the polarization direction of incident light. The measured result for LHCPO is shown in Fig. 8b, where the red and blue curves refer to the cases that light incidents from the forward and backward sides, respectively. The averaged polarization angles are 125.00 and 141.20 mdeg for the forward and backward incidences, respectively, which are indeed originated from the optical activity of LHCPO. Figure 8c shows the simulated polarization rotation angle Δ as a function of φ from LHCPO with substrate, and the averaged polarization angles are 52 and 44 mdeg for the forward and backward incidences, respectively, which meet well with the measured results shown in Fig. 8b. Figure 8d is the simulated polarization rotation angle Δ as a function of φ from LHCPO without substrate, where the averaged polarization rotation angles own the same sign. And this coincides well with the simulated CD signal shown in Fig. 7e, f, indicating the effectiveness of our previous discussions.
In this paper, we have demonstrated that a kind of quasi-3D CPO can be easily achieved through the stepwise colloid sphere lithography technology. Through a four-step deposition process with proper fabrication parameters, the LHCPO and RHCPO are formed on a BK7 glass substrate with CD of ~180 mdeg and optical activity of ~140 mdeg. Due to the advantages of the fabrication method, the CPO with area of larger than 1 cm2 can be easily achieved, and their chiroptical resonance also can be expediently tuned in the infrared and visible region through using proper micro-sphere array and deposition parameters, such as deposition time, angle, and thickness. The FDTD method also has been employed to investigate the chiroptical properties of CPO, and the simulated results meet well with that of the experiment. The in-depth theoretical and experimental researches reveal that the BK7 glass substrate plays an important role in the chiroptical effect of CPO. The substrate can restrain some plasmonic resonance modes and obviously break the mirror symmetry of CPO, leading to a 3D chiroptical effect in the experiment. The CPO with large area, tunable chiroptical spectra, and a low-cost fabrication method could find potential applications in many fields, such as stereochemical enantiomer sensors.
atomic force microscopy
chiral plasmonic oligomers
chiral plasmonic structures
finite difference time domain method
left circular polarization
left-handed chiral plasmonic oligomers
right circular polarization
right-handed chiral plasmonic oligomers
scanning electron microscopy
Thomson WH, Kelvin L (1904) Baltimore lectures on molecular dynamics and the wave theory of light
Shieh J-Y, Kuo J-Y, Weng H-P, Yu HH (2013) Preparation and evaluation of the bioinspired PS/PDMS photochromic films by the self-assembly dip–drawing method. Langmuir 29(2):667–672
Cui Y, Kang L, Lan S, Rodrigues S, Cai W (2014) Giant chiral optical response from a twisted-arc metamaterial. Nano Lett 14(2):1021–1025
Hendry E, Carpy T, Johnston J, Popland M, Mikhaylovskiy R, Lapthorn A, Kelly S, Barron L, Gadegaard N, Kadodwala M (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nat Nanotechnol 5(11):783–787
Tullius R, Karimullah AS, Rodier M, Fitzpatrick B, Gadegaard N, Barron LD, Rotello VM, Cooke G, Lapthorn A, Kadodwala M (2015) “Superchiral” spectroscopy: detection of protein higher order hierarchical structure with chiral plasmonic nanostructures. J Am Chem Soc 137(26):8380–8383
Pendry J (2004) A chiral route to negative refraction. Science 306(5700):1353–1355
Zhaofeng L, Mehmet M, Ekmel O (2013) Chiral metamaterials: from optical activity and negative refractive index to asymmetric transmission. J Opt 15(2):023001
Zhao R, Zhou J, Koschny T, Economou E, Soukoulis C (2009) Repulsive Casimir force in chiral metamaterials. Phys Rev Lett 103(10):103602
Wang H, Zhang X (2011) Unusual spin Hall effect of a light beam in chiral metamaterials. Phys Rev A 83(5):053820
Gao W, Tam WY (2011) Optical activities in complementary double layers of six-armed metallic gammadion structures. J Opt 13(1):015101
Helgert C, Pshenay-Severin E, Falkner M, Menzel C, Rockstuhl C, Kley E-B, Tuennermann A, Lederer F, Pertsch T (2011) Chiral metamaterial composed of three-dimensional plasmonic nanostructures. Nano Lett 11(10):4400–4404
Rogacheva AV, Fedotov VA, Schwanecke AS, Zheludev NI (2006) Giant gyrotropy due to electromagnetic-field coupling in a bilayered chiral structure. Phys Rev Lett 97(17):177401
Polevoy SY, Prosvirnin SL, Tarapov SI, Tuz VR (2013) Resonant features of planar Faraday metamaterial with high structural symmetry study of properties of a 4-fold array of planar chiral rosettes placed on a ferrite substrate. Eur Phys J Appl Phys 61(3):30501
Gansel JK, Thiel M, Rill MS, Decker M, Bade K, Saile V, von Freymann G, Linden S, Wegener M (2009) Gold helix photonic metamaterial as broadband circular polarizer. Science 325(5947):1513–1515
Ma X, Huang C, Pu M, Wang Y, Zhao Z, Wang C, Luo X (2012) Dual-band asymmetry chiral metamaterial based on planar spiral structure. Appl Phys Lett 101(16):161901
Xiaoliang M, Cheng H, Mingbo P, Wenbo P, Yangin W, Xiangang L (2013) Circular dichroism and optical rotation in twisted Y-shaped chiral metamaterial. Appl Phys Express 6(2):022001
Mutlu M, Ozbay E (2012) A transparent 90 degrees polarization rotator by combining chirality and electromagnetic wave tunneling. Appl Phys Lett 100(5):051909
Papakostas A, Potts A, Bagnall DM, Prosvirnin SL, Coles HJ, Zheludev NI (2003) Optical manifestations of planar chirality. Phys Rev Lett 90(10):107404
Esposito M, Tasco V, Cuscuna M, Todisco F, Benedetti A, Tarantini I, De Giorgi M, Sanvitto D, Passaseo A (2015) Nanoscale 3D chiral plasmonic helices with circular dichroism at visible frequencies. ACS Photonics 2(1):105–114
Esposito M, Tasco V, Todisco F, Cuscuna M, Benedetti A, Sanvitto D, Passaseo A (2015) Triple-helical nanowires by tomographic rotatory growth for chiral photonics. Nat Commun 6:6484
Fedotov V, Mladyonov P, Prosvirnin S, Rogacheva A, Chen Y, Zheludev N (2006) Asymmetric propagation of electromagnetic waves through a planar chiral structure. Phys Rev Lett 97(16):167401
Staude I, Decker M, Ventura MJ, Jagadish C, Neshev DN, Gu M, Kivshar YS (2013) Hybrid high-resolution three-dimensional nanofabrication for metamaterials and nanoplasmonics. Adv Mater 25(9):1260–1264
Radke A, Gissibl T, Klotzbuecher T, Braun PV, Giessen H (2011) Three-dimensional bichiral plasmonic crystals fabricated by direct laser writing and electroless silver plating. Adv Mater 23(27):3018–3021
Lan X, Chen Z, Dai G, Lu X, Ni W, Wang Q (2013) Bifacial DNA origami-directed discrete, three-dimensional, anisotropic plasmonic nanoarchitectures with tailored optical chirality. J Am Chem Soc 135(31):11441–11444
Shen X, Asenjo-Garcia A, Liu Q, Jiang Q, García de Abajo FJ, Liu N, Ding B (2013) Three-dimensional plasmonic chiral tetramers assembled by DNA origami. Nano Lett 13(5):2128–2133
Frank B, Yin X, Schäferling M, Zhao J, Hein SM, Braun PV, Giessen H (2013) Large-area 3D chiral plasmonic structures. ACS Nano 7(7):6321–6329
Mark AG, Gibbs JG, Lee TC, Fischer P (2013) Hybrid nanocolloids with programmed three-dimensional shape and material composition. Nat Mater 12(9):802–807
Yeom B, Zhang H, Zhang H, Park JI, Kim K, Govorov AO, Kotov NA (2013) Chiral plasmonic nanostructures on achiral nanopillars. Nano Lett 13(11):5277–5283
Hou Y, Li S, Su Y, Huang X, Liu Y, Huang L, Yu Y, Gao F, Zhang Z, Du J (2013) Design and fabrication of three-dimensional chiral nanostructures based on stepwise glancing angle deposition technology. Langmuir 29(3):867–872
Walther A, Mueller AHE (2013) Janus particles: synthesis, self-assembly, physical properties, and applications. Chem Rev 113(7):5194–5261
Pawar AB, Kretzschmar I (2010) Fabrication, assembly, and application of patchy particles. Macromol Rapid Commun 31(2):150–168
Larsen GK, He Y, Ingram W, Zhao Y (2013) Hidden chirality in superficially racemic patchy silver films. Nano Lett 13(12):6228–6232
Duyne CLHaRPV (2003) Dichroic optical properties of extended nanostructures fabricated using angle-resolved nanosphere lithography. Nano Lett 3(7):939–943
Zhang G, Wang D, Möhwald H (2007) Ordered binary arrays of Au nanoparticles derived from colloidal lithography. Nano Lett 7(1):127–132
Zhang G, Wang D, Möhwald H (2007) Fabrication of multiplex quasi-three-dimensional grids of one-dimensional nanostructures via stepwise colloidal lithography. Nano Lett 7(11):3410–3413
Su Y, Ye S, Hou Y, Shi S, Li S, Gao F, Du J (2014) Design and fabrication of diverse three-dimensional shell-like nano-structures. Microelectron Eng 115:6–12
Kuwata-Gonokami M, Saito N, Ino Y, Kauranen M, Jefimovs K, Vallius T, Turunen J, Svirko Y (2005) Giant optical activity in quasi-two-dimensional planar nanostructures. Phys Rev Lett 95(22):227401
We would like to thank the National Natural Science Foundation of China, Grant No. 61377054.
The authors declare that they have no competing interests.
SX produced the samples, carried out the test of CD spectra and optical activity, did digital simulations, and performed the statistical analysis. SX and YH drafted the manuscript. JY performed part of the simulations. FG and YH participated in the design of the study and made valuable proposals. XX, LP, and JD participated in the analysis and discussion. XL participated in the discussion for the revision. All authors read and approved the final manuscript.