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Fig. 5 | Nanoscale Research Letters

Fig. 5

From: PLGA-PEG Nanoparticles Coated with Anti-CD45RO and Loaded with HDAC Plus Protease Inhibitors Activate Latent HIV and Inhibit Viral Spread

Fig. 5

HDAC4 in ACH-2 cells treated with scFv CD45RO-SAHA NPs or scFv CD45RO-SAHA/Nel NPs was downregulated, leading to HIV-1 reactivation in ACH-2 cells. a Real-time PCR quantification of HDAC4 mRNA expression and b immunoblotting of HDAC4 protein levels on whole-cell lysates from ACH-2 cells cultivated in the presence scFv CD45RO-SAHA NPs, scFv CD45RO-SAHA/Nel NPs, or control (DMEM) at the indicated times. c Real-time PCR quantification of HIV-1 RNA in ACH-2 cells treated with PBS (control), scFv CD45RO-SAHA NPs, or scFv CD45RO-SAHA/Nel NPs for the indicated times. Results were expressed as the fold change vs. the control at each time point (mean ± SEM of three independent experiments)

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