Fig. 2

Simultaneous nanoscale dual-color imaging of CD4 and CD25 on the surface of CD4⁺CD25^low T cells through using NSOM/QD system. a T cell topography. b Fluorescence image of CD4 labeled with QD-655 (red). c Fluorescence image of CD25 labeled with QD-605 (blue). d Merge of CD4 and CD25 two color fluorescence images. e Merge of cell topography and two color fluorescence images. f–h Zoom images of the areas as indicated by the squares on (b–d), respectively. i The percentage numbers of CD4 or CD25 molecules arrayed to form nanodomains. j Molecule density of CD4 or CD 25 nanodomains. Data were expressed as mean ± SEM in (i, j), *P < 0.02, **P < 0.01 (i) and *P < 0.01 (j) compared with control