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Fig. 5 | Nanoscale Research Letters

Fig. 5

From: Nanoscale Relationship Between CD4 and CD25 of T Cells Visualized with NSOM/QD-Based Dual-Color Imaging System

Fig. 5

Simultaneous nanoscale dual-color imaging of CD4 and CD25 on the surface of CD8+CD25+ T cells though using NSOM/QD system. a T cell topography. b Fluorescence image of CD8 labeled with QD-655 (red). c Fluorescence image of CD25 labeled with QD-605 (blue). d Merge of CD8 and CD25 two color fluorescence images. e Merge of cell topography and two color fluorescence images. fh Zoom images of the areas as indicated by the squares on (bd), respectively. i The percentage numbers of CD8 or CD25 molecules arrayed to form nanodomains. j Molecule density of CD8 or CD25 nanodomains. Data were expressed as mean ± SEM in (i, j), *P < 0.02, **P < 0.01 (i) and *P < 0.02, **P < 0.005 (j) compared with control

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