Skip to main content
Account
Fig. 10 | Nanoscale Research Letters

Fig. 10

From: Serum Proteins Enhance Dispersion Stability and Influence the Cytotoxicity and Dosimetry of ZnO Nanoparticles in Suspension and Adherent Cancer Cell Models

Fig. 10

Cellular production of ROS following ZnO NP exposure. ROS generation was evaluated in Jurkat cells at 6, 18, and 24 h following treatment with nZnO/PBS (PBS) and nZnO – FBS/PBS (FBS/PBS) stock dispersions using MitoSOX red and flow cytometry (means ± standard error, minimum of n = 3). Cells were treated with 0.4 mM nZnO and stained with MitoSOX red and CD3 antibody after the desired time. a Mean MitoSOX fluorescence intensity for a non-treated control (NT) and nZnO and nZnO – FBS exposure. To assess relative increases in ROS following NP treatment, a marker (M1) was set so that background fluorescent in control samples (MitoSOX loaded/no NP) was between 1 and 3.5 %. Histograms represent b 6 h, c 18 h, and d 24 h

Back to article page

Navigation