- Nano Idea
- Open Access
Patient-Specific Therapy via Cell-Reprogramming Technology: a Curative Potential for Patients with Diabetes
- Haizhao Luo†1,
- Xianbao Wang†2,
- Ruyi Zhang3,
- Youping Chen1,
- Yi Shu1,
- Huixian Li1 and
- Hong Chen4Email author
© Luo et al. 2015
- Received: 29 July 2015
- Accepted: 12 December 2015
- Published: 29 December 2015
Gene therapeutics provides great opportunities for curing diabetes. Numerous attempts have been made to establish a safe and high-efficiency gene delivery strategy, but all of them are unsuccessful. To achieve an ideal transfection, a novel gene delivery strategy was presented in this research. The novel system proposed was transfection mediated by the combination of ultrasound with microbubbles and cross-linked polyethylenimines (PEIs). Ultrasound with microbubbles enhances the permeability of target cells; moreover, cross-linked PEIs enabled DNA to escape from endosomes into the cytoplasm. If the proposed method is feasible and effective, the endogenous secretion system of insulin would be re-established in patients with diabetes.
In the last two decades, transgenic system based on viral vectors held promises for gene delivery for the efficient delivery and long-term expression of transgenes to target cells . It was documented that viral vectors-mediated transgenes in hepatocytes could ameliorate hyperglycemia in mice [3, 4]. By 2012, viral vectors have been employed as gene carriers in most of researches and clinical trials . However, safety concerns in gene delivery have been highly controversial. Theoretically, viral vectors would induce unexpected immune response and oncogenic effects. In fact, a tragic gene therapy death of a volunteer in a clinical study was caused by administering a high dosage of an adenovirus vector within 98 h . The post-mortem examination revealed that the patient died of multi-organ failure due to the fatal immune response triggered by the administered adenovirus .
Non-viral approaches have also been extensively investigated as alternatives over past a few years. Compared to viral methods, they are likely to present fewer toxic and immunological problems. However, inefficient gene transfer was still their “Achilles heel” currently . It is believed that when only a new non-viral method is explored, the large-scale clinical application of gene therapy would be allowed.
Presentation of the Hypothesis
Given the need, a novel therapeutic strategy using ultrasound (US) with microbubbles technology has entered the realm of gene delivery. Firstly, microbubbles, encapsulating gas in micron-sized shell, were introduced as US contrast agent to improve the ultrasonic imaging . Recent investigations suggested that US with the aid of microbubbles is a potential novel method for the gene therapy in various diseases, both in vivo [10, 11] and in vitro studies [12, 13]. It was addressed that gas-filled microbubbles were driven in US field, and induced shear stresses and sudden ruptures, which subsequently generated force and then punctured on the surrounding cells and tissues, which was termed as sonoporation . Finally, reversible pores resulted from the force enhanced the permeability of the cell membrane temporarily and permitted foreign genes into cells [12, 15, 16]. Besides, endocytosis stimulated by US is another reason for the facilitation of this gene delivery system . Compared with viral methods, many desirable features of gene therapy such as safety, low cytotoxicity, low immunogenicity, and low cost can be found in the gene delivery strategy of US with microbubbles. Whereas this protocol still suffers from the drawback of insufficient transfection efficiency [18, 19].
Cross-linked polyethylenimine (PEI) is a vector tailor-made for gene delivery, which was synthesized by adding 800-Da PEIs to small diacrylate cross-linkers. The degradable polymers exhibited characteristics of similar in structure, size, and DNA-binding properties to off-the-shelf 25-kDa PEI. Notably, they are essentially non-toxic for high efficiencies . Compact nanoparticles are formed through electrostatic attraction between negative charge DNA and positive charge cross-linked PEIs. These particles can escape from the endosomes into the cytoplasm, and prevent DNA from being digested . After reaching the cytoplasm, the particles will decompose into their low molecular-weight components, which are believed to be essentially non-toxic, and then release DNA to enter the nucleus for transcription. Moreover, the synergistic effect of transfection efficiency in combination of US with microbubbles and 25-kDa PEIs was found in Tu and his colleagues’ study . Therefore, we believe that greater transfection efficiency might be achieved via the combination of US with microbubbles and cross-linked PEIs (Fig. 1).
Implications of the Hypothesis
It was proved that transfection with Pdx1, Ngn3, and MafA was a most efficient combination in reprogramming hepatocytes into insulin-producing cells that closely resemble endogenous \( \beta \)-cells in our previous study . It is expected that a safer and more efficient transfection can be achieved by our proposed gene delivery strategy. Laparoscopic approach has been confirmed as a reliable therapeutic option in hepatectomy. Therefore, it is also expected that diabetes could be cured by their own reprogrammed hepatocytes via introducing Pdx1, Ngn3, and MafA (Fig. 2).
The authors would like to thank Eva Xu for her valuable suggestions.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL et al (2000) Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 343(4):230–238View ArticleGoogle Scholar
- Somia N, Verma IM (2000) Gene therapy: trials and tribulations. Nat Rev Genet 1(2):91–99View ArticleGoogle Scholar
- Ferber S, Halkin A, Cohen H, Ber I, Einav Y, Goldberg I et al (2000) Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med 6(5):568–572View ArticleGoogle Scholar
- Kaneto H, Nakatani Y, Miyatsuka T, Matsuoka TA, Matsuhisa M, Hori M et al (2005) PDX-1/VP16 fusion protein, together with NeuroD or Ngn3, markedly induces insulin gene transcription and ameliorates glucose tolerance. Diabetes 54(4):1009–1022View ArticleGoogle Scholar
- Ginn SL, Alexander IE, Edelstein ML, Abedi MR, Wixon J (2013) Gene therapy clinical trials worldwide to 2012—an update. J Gene Med 15(2):65–77View ArticleGoogle Scholar
- Marshall E (1999) Gene therapy death prompts review of adenovirus vector. Science 286(5448):2244–2245View ArticleGoogle Scholar
- Raper SE, Chirmule N, Lee FS, Wivel NA, Bagg A, Gao GP et al (2003) Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer. Mol Genet Metab 80(1–2):148–158View ArticleGoogle Scholar
- Yin H, Kanasty RL, Eltoukhy AA, Vegas AJ, Dorkin JR, Anderson DG (2014) Non-viral vectors for gene-based therapy. Nat Rev Genet 15(8):541–555View ArticleGoogle Scholar
- Lindner JR (2004) Microbubbles in medical imaging: current applications and future directions. Nat Rev Drug Discov 3(6):527–532View ArticleGoogle Scholar
- Shen ZP, Brayman AA, Chen L, Miao CH (2008) Ultrasound with microbubbles enhances gene expression of plasmid DNA in the liver via intraportal delivery. Gene Ther 15(16):1147–1155View ArticleGoogle Scholar
- Chen S, Ding JH, Bekeredjian R, Yang BZ, Shohet RV, Johnston SA et al (2006) Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology. Proc Natl Acad Sci U S A 103(22):8469–8474View ArticleGoogle Scholar
- Li YS, Davidson E, Reid CN, McHale AP (2009) Optimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: potential applications for gene therapy of cancer. Cancer Lett 273(1):62–69View ArticleGoogle Scholar
- Chang S, Guo J, Sun J, Zhu S, Yan Y, Zhu Y et al (2013) Targeted microbubbles for ultrasound mediated gene transfection and apoptosis induction in ovarian cancer cells. Ultrason Sonochem 20(1):171–179View ArticleGoogle Scholar
- Mitragotri S (2005) Healing sound: the use of ultrasound in drug delivery and other therapeutic applications. Nat Rev Drug Discov 4(3):255–260View ArticleGoogle Scholar
- Nomikou N, McHale AP (2010) Exploiting ultrasound-mediated effects in delivering targeted, site-specific cancer therapy. Cancer Lett 296(2):133–143View ArticleGoogle Scholar
- Qiu Y, Zhang C, Tu J, Zhang D (2012) Microbubble-induced sonoporation involved in ultrasound-mediated DNA transfection in vitro at low acoustic pressures. J Biomech 45(8):1339–1345View ArticleGoogle Scholar
- Meijering BD, Juffermans LJ, van Wamel A, Henning RH, Zuhorn IS, Emmer M et al (2009) Ultrasound and microbubble-targeted delivery of macromolecules is regulated by induction of endocytosis and pore formation. Circ Res 104(5):679–687View ArticleGoogle Scholar
- Tsai KC, Liao ZK, Yang SJ, Lin WL, Shieh MJ, Hwang LH et al (2009) Differences in gene expression between sonoporation in tumor and in muscle. J Gene Med 11(10):933–940View ArticleGoogle Scholar
- Kinoshita M, Hynynen K (2007) Key factors that affect sonoporation efficiency in in vitro settings: the importance of standing wave in sonoporation. Biochem Biophys Res Commun 359(4):860–865View ArticleGoogle Scholar
- Forrest ML, Koerber JT, Pack DW (2003) A degradable polyethylenimine derivative with low toxicity for highly efficient gene delivery. Bioconjug Chem 14(5):934–940View ArticleGoogle Scholar
- Pack DW, Hoffman AS, Pun S, Stayton PS (2005) Design and development of polymers for gene delivery. Nat Rev Drug Discov 4(7):581–593View ArticleGoogle Scholar
- Qiu Y, Luo Y, Zhang Y, Cui W, Zhang D, Wu J et al (2010) The correlation between acoustic cavitation and sonoporation involved in ultrasound-mediated DNA transfection with polyethylenimine (PEI) in vitro. J Control Release 145(1):40–48View ArticleGoogle Scholar
- Luo H, Chen R, Yang R, Liu Y, Chen Y, Shu Y et al (2014) Reprogramming of mice primary hepatocytes into insulin-producing cells by transfection with multicistronic vectors. J Diabetes Res 2014:716163View ArticleGoogle Scholar