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Table 2 Gibbs energy of the solution-phase hybridization between the probe mod-Ph and the targets P1, Bcrex14, and TC depending on the buffer stringency

From: SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies

Probe and target

ΔG (kcal/mol)

0.5 × SSC

1 × SSC

2 × SSC

Mod-Ph and P1

−32.2

−33.9

−35.6

Mod-Ph and Bcrex14

−13.9

−14.7

−15.6

Mod-Ph and TC

−4.0

−4.3

−4.6

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