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Table 2 Gibbs energy of the solution-phase hybridization between the probe mod-Ph and the targets P1, Bcrex14, and TC depending on the buffer stringency

From: SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies

Probe and target ΔG (kcal/mol)
0.5 × SSC 1 × SSC 2 × SSC
Mod-Ph and P1 −32.2 −33.9 −35.6
Mod-Ph and Bcrex14 −13.9 −14.7 −15.6
Mod-Ph and TC −4.0 −4.3 −4.6