Table 2 Gibbs energy of the solution-phase hybridization between the probe mod-Ph and the targets P1, Bcrex14, and TC depending on the buffer stringency
Probe and target | ΔG (kcal/mol) | ||
---|---|---|---|
0.5 × SSC | 1 × SSC | 2 × SSC | |
Mod-Ph and P1 | −32.2 | −33.9 | −35.6 |
Mod-Ph and Bcrex14 | −13.9 | −14.7 | −15.6 |
Mod-Ph and TC | −4.0 | −4.3 | −4.6 |