Table 3 The T m values of the solution-phase hybridized duplexes of the probe mod-Ph and the targets P1 and Bcrex14 of the solution-phase determined using the web server DINAMelt and the experimental T m values of the same duplexes formed on the sensor surface
Probe and target | T m calculated (°C) | T m experimental (°C) |
---|---|---|
Mod-Ph and P1 | 64.0 | 64.0 |
Mod-Ph and Bcrex14 | 39.2 | 39.2 |