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Table 3 The T m values of the solution-phase hybridized duplexes of the probe mod-Ph and the targets P1 and Bcrex14 of the solution-phase determined using the web server DINAMelt and the experimental T m values of the same duplexes formed on the sensor surface

From: SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies

Probe and target T m calculated (°C) T m experimental (°C)
Mod-Ph and P1 64.0 64.0
Mod-Ph and Bcrex14 39.2 39.2