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Fig. 4 | Nanoscale Research Letters

Fig. 4

From: Involvement of Ubiquitin-Editing Protein A20 in Modulating Inflammation in Rat Cochlea Associated with Silver Nanoparticle-Induced CD68 Upregulation and TLR4 Activation

Fig. 4

A20+ and RNF11+ cells in the rat cochlea 7 days post-intratympanic injection of 0.4 % AgNPs shown by immunofluorescence confocal microscopy or immunohistochemistry. In the cochleae exposed to dH2O, the spiral ganglion cells (SGCs), inner hair cells (IHCs), pillar cells (PCs), and Deiters’ cells (DCs) of Corti’s organ (CO) showed intensive staining for A20 (j, n), while the strial basal cells (SBCs), spiral ligament fibrocytes (SLFs), and outer hair cells (OHCs) demonstrated mild staining for A20 (d, n). The SBCs, SGCs, and inner pillar cells (IPCs) of CO exhibited intensive staining for RNF11, while the SLFs, hair cells (HCs), and outer pillar cells (OPCs) displayed mild staining for RNF11 (h, l, p). The DCs showed extremely weak staining for RNF11 (p). In the cochleae exposed to 0.4 % AgNPs, the SBCs and SLFs demonstrated more intensive staining for A20 and RNF11 that was independent of the cochlear turn (ac, eg). In CO, the OHCs and DCs displayed more intensive staining for A20 (m), the OPCs and DCs exhibited more intensive staining for RNF11 (o). However, the SGCs and capillary endothelial cells (CaECs) did not show any changes in the staining of A20 and RNF11 (i, k). Comparisons of staining intensity are shown in q and r. Scale bar = 50 μm in ah, 20 μm in m, n, and 30 μm in il, o, p

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