Fig. 1
From: Giant Catalytic DNA Particles for Simple and Intuitive Detection of Pb2+
Schematic illustration of the construction of the 17DS/E DNAzyme microparticles and their catalytic activity. The DNA microparticles containing a large amount of 17E DNAzyme sites were synthesized via rolling circle amplification (RCA) (step 1). The 17DS substrate strands containing a cleavage site were added to the particle solution and then hybridized particles could be formed by denaturation and annealing. The substrate strands are cleaved when Pb2+ is added and the cleaved strands are separated from the particles (step 2)