- Nano Express
- Open Access
Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array
- Jaehyung Lee†1,
- Min-Ho Hong†1,
- Sanghun Han1,
- Jukwan Na1,
- Ilsoo Kim1,
- Yong-Joon Kwon2,
- Yong-beom Lim1Email author and
- Heon-Jin Choi1Email author
© The Author(s). 2016
Received: 21 July 2015
Accepted: 30 May 2016
Published: 22 July 2016
In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
During the past decades, many biosensors have been developed for more sensitive and selective detection of target materials under various conditions. Among these, electrochemical sensors have raised interest due to their simplicity, rapid detectability, and low cost together with high sensitivity . Additionally, they provide direct, specific, and real-time detection of target materials [2–4].
One of the critical factors that determines the performance of electrochemical sensors is the electrode where the reactions occur. Conventional macroelectrodes cannot access micro- and nanoenvironments due to their size. The development of nanoscale electrodes allows for the detection of single biomolecules  and single cell secretions , measurement of local concentration profiles, and in vivo monitoring of neurochemical events . Nanoelectrodes have other advantages regarding diffusion and size. Molecular analytes are consumed at an electrode surface as electrolysis proceeds during an electrochemical redox reaction, making a concentration gradient for fresh analyte to diffuse from the bulk solution. At macroelectrodes, linear diffusion dominates, resulting in limited mass transport. On the other hand, radial diffusion dominates at nanoelectrodes with consequent enhanced rates of analyte mass transport to an electrode. Thus, nanoelectrodes can be used to study faster electrochemical and chemical reactions. In addition, size reduction of each electrode and increase in the total number of electrodes can improve the detection limits and the signal-to-noise (S/N) ratio because the noise level depends on the active area of the individual electrode while the signal depends on the total area of the electrodes .
Several nanomaterials have been studied as electrodes including nanoparticles (NPs) [9, 10], nanobelts (NBs) [11, 12], nanorods (NRs) , and nanotubes (NTs) [14, 15]. Nanoparticles, commonly used for biosensors, show high sensitivity for detection, but their sensing properties often suffer from degradation as a result of the growth of aggregates among the nanoparticles when operated at high temperature for long times . Moreover, difficulties in controlling the particle size deviation and uniform dispersion hinder the development and characterization of nano-integrated devices.
Nanowires (NWs), which can solve these problems, have advantages as building blocks for electrochemical sensors, such as relatively simple preparation methods allowing large-scale production, superior stability due to high crystallinity, and very large surface-to-volume (S/V) ratio. The latter are mandatory for fast reaction kinetics and high density loading of a target species and catalyst deposition over the surface for the induction or inhibition of specific reactions . Thus, one-dimensional (1D) NWs are potential candidates for future sensors.
In this study, we propose an electrochemical biosensor by combining the NWs with artificial peptides designed to recognize HIV-1 Rev response element (RRE) RNA. Developing device aspects to enhance sensor properties, we also used artificial peptides instead of intact proteins. Artificial peptides are economic, easy to synthesize, and are small in size. Additionally, it is easy to change their properties by modifying the side chains. Using the advantages of both the NWs and artificial peptides, we demonstrate ultra-sensitive and -selective detection of HIV-1 RRE RNA.
Reagents and Materials
Rink amide MBHA resin LL and all Fmoc-protected amino acids were purchased from Novabiochem. Fmoc-Ebes-OH was purchased from Anaspec. N-Methyl-2-pyrrolidone was purchased from Merck. Other solvents were purchased from Sigma-Aldrich. Peptide was synthesized on Rink Amide MBHA resin LL using standard Fmoc protocols via a Tribute™ peptide synthesizer (Protein Technologies, Inc.). The peptides were purified by reverse-phase high-performance liquid chromatography (HPLC, water-acetonitrile with 0.1 % TFA). Concentration was determined spectrophotometrically using a molar extinction coefficient of TAMRA (80,400 M−1 cm−1) at 547 nm.
Growth of Silicon Nanowires and Fabrication of Electrodes
The Au-coated vertical silicon nanowire electrode array (VSNEA) was fabricated using the procedures described previously [17, 18] modified as follows. Si (111) substrate was deposited in 0.1 vol.% 3-aminopropyl triethoxysilane (APTES) solution in absolute ethanol. Au catalysts were then coated by immersing the substrate in the colloid solution having Au particles with 250-nm diameter. The substrate was placed in a chemical vapor deposition (CVD) reactor after washing and drying. Silicon nanowires (SiNWs) were vertically grown (with controlled diameter, length, and growth density) on the substrate by flowing SiCl4 and H2 gas. To fabricate the VSNEA, SiNWs were added a metal layer (Au = 80 nm and Ti = 10 nm) and a SiO2 passivation layer onto the surface of the substrate using a sputtering process at a rate of 5 nm/min. Then, the SiO2 passivation layer was selectively etched out to expose the Au tips at the top of the NWs by a complementary metal-oxide semiconductor (CMOS) process.
Peptide Immobilization and RNA Functionalization
The peptide immobilization on VSNEA was carried out by infusing 50 μL of 40 μM peptide in 25 °C environments. The peptide and Au-coated SiNWs were bound by covalent interaction using cysteine (C; this amino acid is expressed in the sequence of peptide in Fig. 4), which has sulfhydryl group, for stability of binding sites. After 12 h for peptide immobilization, RNA was injected to VSNEA using RNase-free water to functionalize with the peptide, and was further incubated for 12 h. Between every immobilization and functionalization step, the VSNEA was washed with distilled water to remove unattached biomolecules.
The cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements were performed with 20 mM potassium ferrycianide in 50 mM phosphate-buffered saline (PBS) solution. CV measurements were carried out in a potential range of 0.8 to −0.8 V and the scan rate was 20 mV/s. Sensitivity and selectivity were measured by DPV with a potential range from −0.2 to 0.6 V, pulse amplitude of 50 mV, and pulse width of 10 ms.
Results and Discussion
Growth of SiNWs and Fabrication of Electrodes
The diameter and length of the SiNWs could be controlled by the size of Au colloidal particles and growth time. The density of SiNWs on the substrate could also be controlled by the concentration of Au nanoparticles in the colloidal solution. Previous studies have shown that the density and size of NWs that are electrodes of a device can change the properties like sensitivity and selectivity of the NW-based sensors .
(NEA; nano electrode array, CONV; conventional electrode).
Equation (5) shows that the S/N ratio of the NEA is higher than that at a conventional electrode because A geom /A act values are generally below 10−3 . This means that detection limits of such devices are two to three orders of magnitude better than that of conventional macroelectrodes . The reaction rate can further be enhanced in the nanowire electrodes since a vertically aligned nanowire can work as an “electron antennae” and enhance the electron transfer for the reactions [23, 24].
Immobilization of RNA onto VSNEA
Detection of Different Concentrations of RNA
The inset of Fig. 5 depicts the linear correlation of the differences in peak currents, [Ip(c) = Ip (c = 0) – Ip (c)] with respect to the target RNA concentration. The regression equation for the peak current (I), and the concentration of the target RNA (c), shows a correlation coefficient (R 2) of 0.99557 with standard deviation of ± 2.310−7 fM. Detection limit was calculated to be 1.513 fM. Recent HIV-1 biosensors using nanomaterials such as nanopore , nanoparticle , and graphene composite film  showed detection limits from tens of fM to nM. Compared to these, the detection limits of VSNEA in this study show superior detectability. This is due to the nanosize and unique shape of nanowire as an electrode, which cause enhanced mass transport and fast electron-transfer kinetics [28, 29]. In addition, total overlap diffusion with proper electrode structure, size, and distance between the electrode elements make it possible to achieve very high sensitivity with fM detection limit.
Detection of Mutated RNA
The working of VSNEA as a biosensor is also explored by the detection of different types of RRE IIB. Three different types [RRE IIB RNA (complementary), RRE IIB mutant and T7 primer DNA] were evaluated by DPV measurement. The sequences used were as follows:
RRE IIB RNA sequence:
RRE IIB mutant sequence:
T7 primer DNA sequence: TAATACGACTCACTATAGGAG
Sensitive and selective detection of HIV-1 RNA by using VSNEA combined with artificial peptides was demonstrated. The advantages of vertical, 1D nanowire electrodes such as high surface area to volume ratio and total overlap diffusion of ions make it possible to detect RNA concentrations of up to 1.513 fM and distinguish between wild-type RNA and mutant RNA. Thus, vertical nanowire sensors may be used as sensitive and selective biosensor platforms in many applications.
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2014M3A7B4051594), the Yonsei University Yonsei-SNU Collaborative Research Fund of 2014, and the Agency for Defense Development (ADD). This work was researched by the third Stage of Brain Korea 21 Plus Project in 2016.
JL and MHH carried out the experiment and drafted the manuscript. SH participated in the biomolecule synthesis. JN, IK, YJK, YbL, and HJC participated in the design of the study and drafted the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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