Polymeric Nanoparticles of Brazilian Red Propolis Extract: Preparation, Characterization, Antioxidant and Leishmanicidal Activity
- Ticiano Gomes do Nascimento1Email author,
- Priscilla Fonseca da Silva1,
- Lais Farias Azevedo1,
- Louisianny Guerra da Rocha2,
- Isabel Cristina Celerino de Moraes Porto1,
- Túlio Flávio Accioly Lima e Moura2,
- Irinaldo Diniz Basílio-Júnior1,
- Luciano Aparecido Meireles Grillo1,
- Camila Braga Dornelas1,
- Eduardo Jorge da Silva Fonseca1,
- Eduardo de Jesus Oliveira3,
- Alex Tong Zhang4 and
- David G. Watson4
© The Author(s). 2016
Received: 29 January 2016
Accepted: 2 June 2016
Published: 17 June 2016
The ever-increasing demand for natural products and biotechnology derived from bees and ultra-modernization of various analytical devices has facilitated the rational and planned development of biotechnology products with a focus on human health to treat chronic and neglected diseases. The aim of the present study was to prepare and characterize polymeric nanoparticles loaded with Brazilian red propolis extract and evaluate the cytotoxic activity of “multiple-constituent extract in co-delivery system” for antileishmanial therapies. The polymeric nanoparticles loaded with red propolis extract were prepared with a combination of poly-ε-caprolactone and pluronic using nanoprecipitation method and characterized by different analytical techniques, antioxidant and leishmanicidal assay. The red propolis nanoparticles in aqueous medium presented particle size (200–280 nm) in nanometric scale and zeta analysis (−20 to −26 mV) revealed stability of the nanoparticles without aggregation phenomenon during 1 month. After freeze-drying method using cryoprotectant (sodium starch glycolate), it was possible to observe particles with smooth and spherical shape and apparent size of 200 to 400 nm. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and thermal analysis revealed the encapsulation of the flavonoids from the red propolis extract into the polymeric matrix. Ultra performance liquid chromatography coupled with diode array detector (UPLC-DAD) identified the flavonoids liquiritigenin, pinobanksin, isoliquiritigenin, formononetin and biochanin A in ethanolic extract of propolis (EEP) and nanoparticles of red propolis extract (NRPE). The efficiency of encapsulation was determinate, and median values (75.0 %) were calculated using UPLC-DAD. 2,2-Diphenyl-1-picryhydrazyl method showed antioxidant activity to EEP and red propolis nanoparticles. Compared to negative control, EEP and NRPE exhibited leishmanicidal activity with an IC50 value of ≅38.0 μg/mL and 31.3 μg/mL, 47.2 μg/mL, 154.2μg/mL and 193.2 μg/mL for NRPE A1, NRPE A2, NRPE A3 and NRPE A4, respectively. Nanoparticles loaded with red propolis extract in co-delivery system and EEP presented cytotoxic activity on Leishmania (V.) braziliensis. Red propolis extract loaded in nanoparticles has shown to be potential candidates as intermediate products for preparation of various pharmaceutical dosage forms containing red propolis extract in the therapy against negligible diseases such as leishmaniasis.
Propolis is a biotechnological product with biological activities produced by bees of the species Apis mellifera from plant exudates which are collected and added to organics or salivary secretions. Propolis has been widely used in alternative and traditional medicine to treat several diseases. The presence of secondary metabolites from plants like phenolic acids, phenolic esters, flavonoids, clerodanes, lupeones, propolones, prenylated benzophenones have been identified in different propolis around the world and are responsible for the biological activities in propolis raw material . These compounds in whole are considered a “multiple-constituent extract” from a natural product and has a multi-target purpose in biological systems .
Propolis have presented diverse pharmacological activities amongst them antioxidant , antimicrobial [4, 5], antifungal [6, 7], antiviral , antiparasite [8, 9], anti-inflammatory [3, 4], anticancer [1, 10–16] and wound healing [17, 18], but its biological activity depends on its particular chemical composition and some favourable conditions such as bee species and its genetic variability, geographic area, biodiversity of plant species around hives, climate and sazonality and presence of water around the hives specially in semiarid area.
Recent studies that sought to characterize Brazilian red propolis found molecules, such as elemicin, isoelemicin, methyl isoeugenol, methyl eugenol, formononetin, biochanin A, isoliquiritigenin, liquiritigenin, medicarpin, homopterocarpan, quercetin and vestitol, that allow it to be distinguished from other types of Brazilian propolis [3, 19, 20]. The red propolis present the unique characteristics of chemical composition and multiple biological activities in addition to standardized production in the areas of mangroves swamps of Alagoas, Brazil, respecting a environment conservation policy of the Atlantic rainforest. Then, the production of red propolis raw material and hydroalcoholic extract is part of a production chain of apiceuticals and bioproducts that promotes sustainable development in the region of mangroves and lagoons being awarded the geographical indication label with the designation of origin.
Nanoparticles have been studied since early 1990s and used as drug nanocarriers and are be able to be encapsulated inside nanocarriers or onto the surface of small nanocarrier molecules, genes, biopharmaceuticals and diagnostic and imaging agents [21–23]. The nanocarriers commonly present different matrix systems (nanostructured materials) and an architecture composed of polymers (polymeric nanoparticles), liposomes (solid lipid nanoparticles, cubosomes, niosomes, spherulites etc.) [21, 22, 24], metal nanoparticles (silicon nanoparticles, gold nanoparticles, silver nanoparticles, magnetic nanoparticles), carbon nanotubes and quantum dots (diagnostic image agents) [23, 25–27].
But polymeric nanoparticles and liposomes are amongst the most accepted delivery vehicles of drugs with clinical applications in pharmaceutical area and approved as generally recognized as safe (GRAS). Polymeric nanoparticles reached this status due to biocompatibility, non-toxicity to biological systems, biodegradability, stability during storage, controlled release, target delivery, resulting in higher therapeutic efficacy [21, 25, 28, 29].
Nanoparticles can be prepared by different techniques including solvent emulsification-evaporation, high pressure homogenization, high-speed stirring, ultrasonication, microemulsion using spray-drying, nanoprecipitation and others [29–31]. Preparation and characterization of nanoparticles loaded with isolated drugs , vaccines  or isolated phytomedicine (kampferol, luteolin, cathechin, quercetin) [34, 35] and propolis extract  are applied in cancer therapy [37–39] and other diseases  including a negligible disease such as leishmaniasis [41, 42]. However, there are few papers describing herbal extract encapsulated in nanoparticles [43–49]. Few studies describe the use of propolis extracts with effective leishmanicidal effect . There is also a current trend in the use of conventional therapy using the drug of first choice in association  or drug of first choice combined with phenolic compounds for treatment of various diseases resulting in enhancement of therapeutic efficacy or reducing the side effect of drugs used in the therapy of diseases such as leishmaniasis .
There is a great diversity in a nanoparticulate system to transport for target tissues the drug-drug or drug combined with phytochemicals [53, 54]. The development of “multi-compound extract in co-delivery system” for target tissues arises due to the high prevalence of the number of cases of cancers  and the increase in drug-resistance during leishmaniasis treatment worldwide [56–59].
The use of bee products and propolis comes from ancient times to present days, and there is an increasing interest to standardize apiceutical products for use in the pharmaceutical and cosmetic industries. It is necessary to carry out complex and diverse scientific studies of dose, composition, formulation stability and therapeutic activity of this complex mixture of substances of different phytochemical classes to ensure the efficacy, safety, quality and productivity. Currently, pharmaceutical industries have extensively explored the use of nanoparticles for “drugs in co-delivery system” in therapy against various diseases especially for the treatment of cancer as well as cardiovascular diseases, metabolic syndrome diseases and also negligible diseases. Thus, this study aimed to explore the potential of the propolis extract loaded in polymeric nanoparticles containing a pool of flavonoids and phenolic compounds which may act in a synergistic action for treating chronic and neglected diseases previously such as leishmaniasis.
The aim of the present study was to prepare and characterize polymeric nanoparticles loaded with Brazilian red propolis extract and access the antioxidant and leishmanicidal activity of “multiple-constituent extract in co-delivery system” present in red propolis extract loaded in a nanoparticulate system.
Red propolis raw material (150 g) was collected from Marechal Deodoro city, state of Alagoas, Brazil. Propolis was collected from the Ilha do Porto apiary with the following geographical coordinates: south latitude 9° 44.555′, west latitude 35° 52.080′ and height of 18.1 m above sea level. The access and transportation of Brazilian red propolis was previously authorised by regulatory agencies controlling Brazilian Genetic Heritage and Biodiversity Conservation with protocol number of acceptance 010124/2012-8.
Poly-ε-caprolactone (P.M 10.000), pluronic F-108 copolymer (poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (P.M. 14.000), and 2,2-diphenyl-1-picryhydrazyl (DPPH) reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Analytical standards: pinobanksin, isoliquiritigenin, formononetin and biochanin A were purcharsed from Sigma-Aldrich (St. Louis, MO, USA), and liquiritigenin was acquired from Extrasynthese (Lyon, France). HPLC-grade acetonitrile was purchased from J. T. Baker Mallinckrodt-Avantor (NJ, USA). Schneider for insect medium was purchased from Sigma-Aldrich (St. Louis, MO, USA). Biphasic medium Schneider’s/Novy-McNeal-Nicolle, supplemented with 10 % of foetal bovine serum (FBS), 50 U of penicillin/mL and 50 μg of streptomycin/mL was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Preparation of Ethanolic Extract of Propolis (EEP)
Raw propolis (250 g) was manually grounded and placed in a flask with 600 mL of 80 % ethanol, which was placed on an agitator (Thornton, Model T14, USA) for 48 h. Then, the macerate (the liquid portion) was removed using a pipette, and the solid portion (wax) was discarded. The macerate was mixed with 600 mL of 80 % ethanol in a glass flask and placed on the agitator for 24 h. Then, the resulting macerate was mixed again with 600 mL of 80 % ethanol and left for 24 h without agitation.
Next, the macerate was removed using a pipette, filtered through filter paper and subjected to distillation under reduced pressure in a rotary evaporator (Fisatom, São Paulo, Brazil) in a water bath at temperature 40–50 °C, pressure 650 mmHg and speed 80 rpm to remove the solvent. The EEP was then placed in a glass container and left for approximately 3 days for the residual solvent to evaporate; as a result, a solid mass (162 g) with viscous appearance was obtained.
Preparation of Nanoparticles Loaded with Nanoparticles of Red Propolis Extract (NRPE)
Compositions of nanoparticles loaded with red propolis extract (NRPE) using a nanocarrier in matrices system PCL-pluronic
Red propolis extract
The colloidal suspensions of nanoparticles were obtained by the preformed polymer interfacial deposition method also called nanoprecipitation preformed polymer . The components of the organic and aqueous phases were weighed, placed in individual beakers and subjected to sonication until complete dissolution (15 min). Then, the organic phase (500 μL) was poured into the aqueous phase (50 mL) and vortexed for 1 min. The suspensions of polymeric nanoparticles containing red propolis extract (NRPE) were submitted to the characterization assays.
The NRPE were centrifuged for 15 min under a rotation speed of 5000 rpm in a small centrifuge for Eppendorf tubes (MiniSpin model) to form a solid pellet of non-rigid consistency in the bottom of the Eppendorf tube, which was later transferred to a 10-mL vial. The supernatant, containing acetone and water, was discarded, and nanoparticles were diluted with milli-Q water (1:5, v/v) and subjected to freeze-drying using three methods of drying. The suspension of nanoparticles were dried at room temperature (method A), by slow freeze method in freeze at −20 °C (method B) and by fast freeze method using liquid nitrogen (method C) to form the solid nanoparticles. The same procedure was performed for placebo composition (nanoparticles without red propolis extract, NRPE placebo).
Freeze-Drying of Nanoparticles Loaded with Red Propolis Extract
The suspensions of red propolis nanoparticles were submitted to two freezing-drying processes: (1) slow freezing, in which the suspensions of red propolis nanoparticles were placed in a freezer at −20 °C for a period between 48 and 120 h and immediately transferred to freeze-dryer to perform the drying process for a period between 24 and 72 h (method B); and (2) fast freezing with liquid nitrogen at −196 °C for a period between 10 and 20 min and immediately transferred to a freeze-dryer to perform the drying process for a period between 24 and 36 h (method C).
Cryprotectant agents such as colloidal silicon dioxide from 0.1 to 10 %, sucrose between 1 and 15 %, glucose between 1 and 15 %, a solution containing combination of colloidal silicon dioxide and sodium starch glycolate (1:1, w/w) in concentration ranges between 10 and 30 % and sodium starch glycolate solution from 0.10 to 30 % were used.
The freeze-dryer used to dry the red propolis nanoparticles was a Terroni equipment®, LD 1500 model (São Paulo, Brazil), which comprises three shelves in a drying chamber, a condenser to −43 ± 5 °C and a vacuum pump. The equipment used for freeze-drying showed temperature stability and low pressure in the condenser. The system pressure remained below 300 μHg when the suspensions of nanoparticles are presented in solid state.
The EEP and nanoparticles loaded with red propolis extract were subjected to physicochemical characterization, investigation of antioxidant activity and leishmanicidal assay using Leishmania (V.) braziliensis species.
Characterization of Suspension of Nanoparticles Loaded with Red Propolis Extract
Particle size, Zeta Potencial and pH
The determination of average diameter and polydispersion index of nanoparticles in suspension were made using dynamic light scattering. The suspensions were fourfold diluted in Milli-Q water and analysed in Zetasizer apparatus, model Nano-ZS from Malvern. The results were determined by the average of two cycles of 20 scans. The zeta potential of the nanoparticles suspension were obtained by the eletrophoretic mobility technique in the zetasizer apparatus in which the samples were diluted fourfold with Milli-Q water and results were expressed in millivolts (mV) from the average of two cycles of 20 scans. The nanoparticles pH determinations were performed using a previously calibrated glass electrode, and the measurements were carried out directly from nanoparticles suspension without any dilution.
Solid State Characterization of Red Propolis Extract Loaded in Nanoparticles
The calorimetric curves of polymeric nanoparticles containing red propolis extract were obtained in a differential scanning calorimeter, model DSC 50 from Shimadzu (Tokyo, Japan), using a mass of (5.0 ± 10 % mg), which were packed in aluminium capsules hermetically sealed. The heating rate was 10 °C min−1 in the temperature range 30–400 °C under an atmosphere of nitrogen and flow rate of 50 mL min−1. The DSC was calibrated using indium and zinc standards according to the Shimadzu suggested procedures.
The nanopolymeric system loaded with propolis extract in solid state was subjected to attenuated total reflectance (ATR)-FTIR analysis, which was performed by ATR in the wavenumber range 4000 to 400 cm−1 and 64 scans. The results were expressed in infrared transmittance percentage. The equipment used was a Thermo Scientific coupled to the Ommic software for data acquisition. EEP and NRPE including placebo were analysed.
Scanning electron microscopy images were analysed to confirm the morphology and approximate size of nanoparticles in solid state. The compositions of red propolis nanoparticles were fixed on stubs with double carbon tape and covered by a gold film during the metallization process with 10 mA for 7 min in a System Sanyu Electron, Quick Coater Model SC-701. SEM micrographics have been taken from Shimadzu microscope (SSX-550 Superscan model).
Determination of markers in Polymeric Nanoparticles Using UPLC-DAD
The identification and quantification of flavonoids in EEP and NRPE (in solid state) were performed using a ultra performance liquid chromatography coupled with diode array detector (UPLC-DAD) from Shimadzu consisting of the following modules: a high-pressure pump (Model LC-20ADXR), degasser (model DGU-20A3R), Autoinjector (model SIL-20AXR), oven chromatographic column, photodiode array detectors (model EPDM-20A) and fluorescence detector (RF-20A model), a controller (model CBM-20A) and a Shimadzu Labsolution software. The separation of flavonoids occurred using a reversed-phase column (C18, 150 × 4.6 mm; 5 μm), a mobile phase that consisted of solvent A (Milli-Q water) and solvent B (acetonitrile), pumped at a flow rate of 0.3 mL/min. The initial elution gradient consisted of water (70 %) and acetonitrile (30 %) with a variation of the percentage of acetonitrile to 100 % in 40 min followed by an isocratic condition with acetonitrile (100 %) up to 53 min and return to the initial condition at 54 min, followed by isocratic conditions acetonitrile (30 %) up to 60 min. This long method was developed in order to wash the column during the analysis with 100 % of acetonitrile and avoid lack of accuracy and precision during the efficiency of entrapment assay and to avoid column fouling and excessive pressure buildup by irreversible retention of non-polar compounds (terpenes and guttiferones present in red propolis extract).
Analytical standards were exactly weighed (2.0 mg) transferred to 10-mL volumetric flasks to obtain a concentration of 200 μg/mL. The working solutions were diluted to obtain the final calibration curve concentrations of 7.50, 5.00, 2.50, 1.00, 0.50 and 0.15 μg/mL. These calibration curves were used for determining the content (assay) of flavonoids in the extract (EEP) and NRPE loaded with red propolis extract. The identification of flavonoids was performed by retention time comparison with analytical standards using the same analytical conditions on the same working day.
Efficiency of Encapsulation (%)
The efficiency of encapsulation was also performed using these same analytical conditions and with five markers (liquiritigenin, pinobanksin, formononetin, isoliquiritigenin, biochanin A) present in the EPP, which were determined in NRPE following the same separation conditions. The propolis extract (100 mg) as a solid (<1 % solvent) was solubilized in absolute ethanol with ultrasonic bath (5 min) and transferred to a volumetric flask (10 mL) to obtain a concentration of 10 mg/mL. A further dilution step was performed to obtain working solution of 1 mg/mL, and then final dilutions at 250 μg/mL were performed. These solutions were filtered through filter units 0.22 μm, and (2 μL) were injected in the UPLC-DAD system.
Antioxidant Activity of the Polymeric Nanoparticles Using DPPH Method
Quantitative assessment of the antioxidant activity of EEP and NRPE were performed according to the methods described in the literature  with few modifications. The inhibition of free radical DPPH by the samples was monitored by measuring the decrease in absorbance of solutions with different concentrations. The solvent ethanol was used as blank. A solution of DPPH (3 mM) was prepared transferring 0.0118 g of DPPH reagent to a 100-mL volumetric flask with ethanol.
The EEP and NRPEs were prepared at an initial concentration of 1.0 mg/mL in the solvent system acetone/ethanol (6:4, v/v). An aliquot of 400 μL was transferred to a volumetric flask of 5 mL, and then 2.0 mL of DPPH solution (3 mM) was added and diluted with ethanol until achieving final concentrations of 80.0 μg/mL. The reaction was left to develop in the dark at room temperature (25 °C) over 30 min. The absorbance readings were then performed with a spectrophotometer (Model UV-1240, Shimadzu, Kyoto, Japan) at 518 nm.
In vitro Biological Assays
L. (V.) braziliensis culture
The strains of L. (V.) braziliensis (IOC-L0566 - MHOM/BR/1975/M2903) from FioCruz (Recife, Brazil) [62, 41] promastigotes were cultured in vitro in biphasic medium Schneider’s/Novy-McNeal-Nicolle, supplemented with 10 % of FBS, 50 U of penicillin/mL and 50 μg of streptomycin/mL, incubated in a BOD camera at temperature of 26 °C.
Antileishmanial In vitro Assay
Sample preparation: previously, EEP and NRPE were exactly weighed and solubilized with DMSO/H2O (Milli-Q water) (75:25,v/v) to obtain a stock solution of 10,000 μg/mL. Using this solvent system, EEP was completely solubilized but NRPE was only partially solubilized. Several work solutions were obtained transferring aliquots of EEP or NRPE stock solution and diluting with Schneider’s medium supplemented with 10 % BFS to obtain volumes of 1 mL to each concentration at the range between 1000 and 5 μg/mL. In this way, the concentration of DMSO solvent did not exceed 0.5 %, and at this concentration, DMSO had no deleterious effect to the parasites .
Antileishmanial activity was carried out according to Rocha et al. . Aliquots of (100 μL) of the cultured procyclic promastigotes cells of the parasite (at a concentration of 4 × 106 cells/mL) immersed in the Schneider’s medium with 10 % BFS in 96-well microplates were incubated for a 24-h period in BOD camera at 26 °C with 100 μL of samples (EEP and NRPE) at the concentrations of 1000, 500, 400, 350, 300, 250, 200, 160, 100, 80, 60, 50, 40, 30, 20, 10 and 5 μg/mL. Afterwards, the morphology and parasites viability were evaluated in an inverted microscope (Olympus - IX70) to all concentrations tested, and then the parasite number was determined through optical microscope in the ×40 objective using a Neubauer chamber to express the antileishmanial effect. The results were expressed as the mean parasite growth inhibitory concentration (IC50).
All results are presented as mean ± standard deviation for, particle size, size distribution, zeta potential of NRPE, (%) efficiency of entrapment (n = 3) using UPLC-DAD and antioxidant activity by DPPH method. The mean ± standard deviation of the mean (SDM) relative to the percentage (normalized graphs) of cell growth inhibition and parasite growth inhibition were used to estimate the IC50 using non-linear regression with the software GraphPad Prism, version 5.0. The significance level was set as p < 0.05.
Results and Discussion
Characterization of Suspension of Nanoparticles Loaded with Red Propolis Extract
Particle Size, pH and Zeta Potential
Particle size and polydispersion index and zeta potential and pH of nanoparticles loaded with red propolis extract in suspension
Particle size (nm)
Polydispersion index (PDI)
Zeta potential (mV)
279.6 ± 1.4
−33.5 ± 6.06
6.0 ± 0.1
280.2 ± 8.7
−26.8 ± 4.64
6.1 ± 0.1
262.2 ± 6.60
−18.6 ± 3.66
6.1 ± 0.2
208.5 ± 4.79
−12.7 ± 5.24
5.8 ± 0.2
225.2 ± 7.72
−24.2 ± 5.24
6.0 ± 0.1
246.7 ± 9.2
−19.1 ± 4.61
6.1 ± 0.1
Values of particle size in the nanometric scale varying between 208.5 and 280.2 nm with polydispersity index from 0.089 to 0.169 demonstrating unimodal model, i.e., monodisperse particles. The pH of the suspensions of nanoparticles (NRPE) was similar to the placebo with slightly acidic values between 5.83 and 6.15.The slightly acidic characteristic of suspension of nanoparticles can be attributed to the presence of the PCL polymer and the greater number of carboxyl end groups , and pH values between 4.6 and 6.0 were due to PCL polymer . The pH is an important parameter to evaluate the stability of the nanoparticles in suspension and can be an indicator of polymer degradation or drug diffusion to aqueous medium.
The zeta potential varied from −18.6 to −26.8 mV, except NRPE A3 (−12.7 mV). Changes in the size of nanoparticles may be influenced by the nature and concentration of the polymer in the organic phase, polarity of solvent, the nature and concentration of the surfactants in the aqueous phase . Chawla and Amiji  observed changes in particle size after removal of the pluronic F-68 surfactant with a significant increase in particle size. Therefore, the presence of pluronic in the nanoprecipitation process acts as a dispersant and also as a stabilizer. Solid materials have electric charge on its surface, after contact with a liquid. The high values of the zeta potential, either negative or positive (±30 mV), represent the achievement of a stable suspension, by repulsion between particles which prevents the occurrence of aggregation of the nanoparticles. The suspensions of nanoparticles were stable and dispersed, and no tendency to form aggregates was observed. In our studies, we monitored the stability of the suspensions of nanoparticles during 30 days and no aggregations or precipitation was observed, except for NRPE A3. Only NRPE A3 has developed precipitation after 10 days of storage.
Our strategies were prepare suspensions of nanoparticles or solid lipid nanoparticles loaded with red propolis extract and then incorporate it in cosmestic compositions (semisolid composition like cosmestic creams). Thus, these zeta potential values were sufficient to stabilize the nanoparticles of red propolis extract. Negative values for zeta potential are a desired condition to promote the permeation of molecules across the skin barriers especially for transdermal compositions. Nair et al.  reported that slightly negative or positive charges on the surface of the particles can control parameters such as the solubility and stability, as well as prevent loss of nanoparticles in undesired locations. In this way, it is a sine qua non condition for this natural product to maintain the multi-target purpose in biological systems. This stability of the suspensions of nanoparticles for our strategy (semisolid composition like cosmetic creams) is sufficient to stabilize the nanoparticles of red propolis extract.
Characterization of Red Propolis Nanoparticles in Solid State
The accompanying Fig. 1 shows DSC thermograms of the propolis extract, the polymeric coating matrix (PCL-pluronic) and NRPE compositions. The DSC thermogram of the red propolis extract showed four endotherm events at temperatures of 81.7, 92.0, 107.0 and 135.0 °C. Three endothermic peaks between 81.7 and 107.0 °C correspond to water volatilization, and the fourth endothermic peak at 135 °C refer to fusion processes of low molecular weight compounds like flavonoids in mixture and other phenolic compounds present in the propolis extract.
Figure 3a, b (NRPE A1 and NRPE A1) was obtained by the method of drying at room temperature (natural drying). Figure 3a, b shows photomicrographs of submicron particles (between 450 and 960 nm) obtained by drying process at room temperature (method A) with the formation of nanocrystals, and Fig. 3c, d shows scanning electron microscopy of NRPE A2 like submicron particles (between 400 and 960 nm) with the formation of amorphous aggregates when obtained using drying method B (slow freezing at freezer −20 °C).
Figure 3 shows scanning electron microscopy of NRPE A4 obtained by method C of drying. Micrographs with magnification of ×7000 (scale 2 μm) and magnification up to ×10,000 (scale 1 μm) showing the polymeric nanoparticles loaded with red propolis extract. Figure 3e, f (NRPE A4) was obtained by drying method C. Figure 3e shows a micrograph of the nanoparticles (200 and 350 nm) using colloidal silicon dioxide as cryoprotectant, and Fig. 3f shows a photomicrograph of the nanoparticles (between 175 and 320 nm) using a sodium starch glycolate as cryoprotectant. Method C presented spherical nanoparticles in nanometric scale and no aggregates.
Determination of markers in Polymeric Nanoparticles Using UPLC-DAD
Determination of the markers present in EEP and nanoparticles loaded with red propolis extract using UPLC-DAD
Concentration of flavonoids(μg/mL) ± SDa
2.290 ± 0.760
0.330 ± 0.003
2.510 ± 0.010
2.960 ± 0.010
0.371 ± 0.010
1.240 ± 0.040
0.329 ± 0.004
2.400 ± 0.040
1.740 ± 0.010
0.370 ± 0.004
0.950 ± 0.010
0.238 ± 0.020
1.770 ± 0.020
1.740 ± 0.010
0.324 ± 0.003
0.820 ± 0.01
0.200 ± 0.004
2.032 ± 0.001
1.860 ± 0.010
0.318 ± 0.001
1.216 ± 0.004
0.240 ± 0.003
1.888 ± 0.001
2.032 ± 0.022
0.327 ± 0.002
1.168 ± 0.003
0.230 ± 0.001
1.792 ± 0.031
2.002 ± 0.013
0.321 ± 0.002
Efficiency of Encapsulation (%)
Efficiency of encapsulation (%) of flavonoid marker compounds present in the nanoparticles loaded with red propolis extract
(%) Efficiency of encapsulation of flavonoids using UPLC-DADa
53.0 ± 1.6
99.8 ± 1.7
95.6 ± 1.7
77.4 ± 1.7
99.8 ± 1.7
41.3 ± 0.4
72.0 ± 0.9
70.5 ± 0.9
58.6 ± 0.5
82.1 ± 1.3
38.0 ± 0.8
60.9 ± 1.3
103.6 ± 0.7
75.4 ± 0.6
85.7 ± 0.2
57.3 ± 0.3
72.9 ± 0.8
96.6 ± 0.1
82.2 ± 1.4
88.3 ± 1.7
54.9 ± 0.2
70.9 ± 0.2
91.5 ± 2.5
81.2 ± 0.8
86.4 ± 1.7
Efficiency of encapsulation (%) and non-encapsulation proportion (%) of the flavonoid marker compounds present in nanoparticles of red propolis extract
(%) Efficiency of encapsulation of flavonoids using UPLC-DAD
∑ (%E.E. + %N.E.)
Generally, an apiceutical extract is a “multiple-component agent” and presents different chemical classes of compounds like phenolic acids, esters, flavonoids and terpenes, and each chemical class display a particular chemical reactivity and is likely to react with light, air, water and other extrinsic factors promoting rapid degradation or inactivation of biological activity. The polymeric encapsulation of red propolis extract was a successful strategy to protect this apiceutical extract against degradation and inactivating itself; besides, this encapsulation process was able to store the red propolis extract during 9 months in solid-state form.
Antioxidant Activity of the Polymeric Nanoparticles Using DPPH Method
Antioxidant activity (%AOA) of EEP and its nanoparticles loaded with red propolis extract
∑(%E.E. + %N.E.)
81.40 ± 2.75
30.73 ± 1.04
76.63 ± 6.86
27.18 ± 0.95
79.51 ± 2.13
36.75 ± 1.33
77.35 ± 2.98
38.10 ± 0.36
76.22 ± 5.74
18.27 ± 1.77
In vitro Biological Assays
The EEP and NRPE presented antileishmanial activity proven by IC50 values. The EEP showed an IC50 of 37.9 μg/mL (CI 95 % 33.57–43.11 μg/mL), and NRPE samples showed an IC50 of 31.34 μg/mL (CI 95 % 27.24–35.71 μg/mL) (NRPE A1), 47.23 μg/mL (CI 95 % 42.41–53.23 μg/mL) (NRPE A2), 154.2 μg/mL (CI 95 % 140.60–169.20 μg/mL) (NRPE A3) and 193.2 μg/mL (CI 95 % 187.60–201.40 μg/mL) (NRPE A4) (Fig. 5A). In general, the compositions of EEP-loaded into nanoparticles comprising 30 and 40 % of EEP maintained antileishmanial activity similar to the EEP in its original form. Leishmanicidal activity of the prepared nanoparticles has not been found to be a concentration-dependent response but is related to efficiency of encapsulation and ratio between polymeric matrix and extract of red propolis present in the nanoparticles. The NRPE A4 composition demonstrated the lowest amount of EEP and had the lowest leishmanicidal activity of these compositions. In general, the polymeric matrix (PCL-pluronic) has shown to be a controlled release system very efficient for release of drugs, and in this particular composition (NREP A4), the polymeric system served as a delayed release system by reducing the release of flavonoids present in the propolis extract against L. braziliensis. The NRPE A3 composition presented highest loss for markers liquiritigenin and formononetin, and the highest amount of markers determined outside of nanoparticles was observed (Tables 4 and 5).
Propolis extracts from different part of the world have been investigated against leishmania parasites and has demonstrated relevant leishmanicidal activity. Duran et al.  showed antileishmanial activity for two types of Turkey propolis extracts (propolis Hatay and Bursa propolis) and demonstrated an IC50 of 250 and 500 μg/mL. Another relevant study by Duran et al.  revealed good results for Adana propolis against leishmania parasite in concentrations above 250 μg/mL. Another type of Turkish propolis extract (Kayseri propolis) studied by Ozbilge et al.  showed excellent leishmanicidal activity against Leishmania tropica with an IC50 of 32 μg/mL.
Comparative study of the green propolis extracts from Brazilian and Bulgarian propolis extract carried out by Machado et al.  showed leishmanicidal activity against four different species of Leishmania (amazonensis, braziliensis, chagasi and major). Brazilian green propolis extract showed IC50 close to 49 μg/mL against L. braziliensis, L. chagasi and L. major species, while the Bulgarian propolis extract showed leishmanicidal activity for L. amazonensis, L. chagasi and L. major species with IC50 between 2.8 and 41.3 μg/mL. Excellent leishmanicidal activity of Bulgarian propolis can be explained by the presence of various flavonoids present in its extract as pinobanksin esters, pinocembrine, chrysin, some phenolic acids and phenolic acid esters . Brief description of the scientific literature shows that some flavonoid aglycones such as quercetin, luteolin and fisetin have high leishmanicidal activity with IC50 values in the range from 0.6 to 0.8μg/mL. Flavonoid glycosides such as rutin, quercitrin and isoquercitrin have also demonstrated leishmanicidal activity. Scientific research by Da Silva et al.  showed that the Brazilian green propolis displays leishmanicidal activity in concentrations ranging from 5 to 100 μg/mL in in vitro cytotoxicity studies at the times 24, 96 and 168 h. Miranda et al.  demonstrated efficacy of combined therapy of complex Ru-NO (NO donor) and Brazilian green propolis extract in the treatment of American cutaneous leishmaniasis (ATL).
Brazilian red propolis extract with high concentrations of prenylated benzophenones has been evaluated in macrophages infected with L. amazonensis and demonstrated that a concentration of 25 μg/mL is capable of increasing the reductive activity of MTT being active against these intracellular parasites present in macrophages . In our studies, the hydroalcoholic propolis extract showed antileishmanial activity against L. braziliensis with IC50 values ≅38.0 μg/mL, and the same results were obtained with polymeric nanoparticles loaded with propolis extract with IC50 values between 31.3 and 47.2 μg/mL for NRPE A1 and NRPE A2, respectively.
We have developed a method to evaluate only biological activity of nanoparticles (NRPE) against the parasite L. (V.) braziliensis that is a biological quality control method for these nanoparticles, and it is considered as an intermediary product (bulk form) during the production of semisolids (cosmetic creams). In our experiments, we proved similar efficacy of NRPE (nanoparticle loaded with red propolis) to EEP (ethanolic extract of red propolis) for NRPE A1 and NRPE A2, and these nanoparticles will deserve further development efforts aimed at a final composition (cosmetic cream) against cutaneous leishmaniasis. Previous studies using emulsionated curcumin  and phospholipid nanoparticles of ursolic acid  obtained using preparation techniques such as emulsification-evaporation and amphiphilic self-assembly was shown to affect parameters such as absorption, half-life and bioavailability when compared to the effect of the isolated substances. Thus, in the final formulation excipients could be taylored to modulate and improve pharmacokinetic parameters of natural products .
Our chormatographic study determined the presence of some isoflavonoids (formononetin, biochanin A), dyhydroflavonol (pinobanksin), chalcone (isoliquiritigenin) and flavonone (liquiritigenin), but formononetin, isoliquiritigenin and liquiritigenin were considered the major flavonoids present in the EEP and also in NRPE. It was previously shown that the presence of isoliquiritigenin, a chalcone, and other flavonoids and isoflavonoids present in EEP and in the prepared nanoparticulate extract (NRPE) can inhibit the activity of L. (V.) braziliensis through different biochemical targets such as cytoplasmic membrane and the mitochondrial respiratory complex. The propolis extract present a multitude of constituents that can act on multiple biochemical targets affecting the balance of complex cellular networks in a way that is currently not fully understood such as the multi target efficacy model proposed by CSERMELY et al. .
Recent studies showed that flavonoid chalcones have high activity in inhibiting the growth of parasites including Leishmania. Torres-Santos et al.  demonstrated that naturally occurring chalcones (dihydromethoxylated chalcone) alter the biosynthesis of sterols in L. amazonensis and promote ergosterol accumulation and cholesterol decrease. This alteration resulted into increase the membrane fluidity and toxic effect to the parasite with IC50 of 5.5 μM. Previous studies have shown a similar mechanism of action for amphotericin B (leishmanicidal drug) .
Nanoparticles (200-800nm) loaded with red propolis extract were prepared and characterized both as suspensions and in solid state form, based on PLC-pluronic matrix system. This copolymeric matrix system was able to encapsulate different flavonoids from red propolis extract with particular characteristics of solubility and antioxidant activity.
Nanoparticles loaded with red propolis extract in multidrug co-delivery system and EEP presented cytotoxic activity on L. braziliensis. Red propolis extract loaded in nanoparticles was shown to be a potential candidate as intermediate products for preparation of various pharmaceutical dosage forms containing red propolis extract in the therapy against neglected diseases such as leishmaniasis.
%AOA, percentage of antioxidant activity; DMSO, dimethyl sulphoxide; EEP, ethanolic extract of propolis; NRPE, nanoparticles of red propolis extract; DPPH, 2,2-diphenyl-1-picryhydrazyl; UPLC-DAD, ultra performance liquid chromatography coupled with diode array detector.
The authors thank the Federal University of Alagoas, FAPEAL, CAPES and CNPq for the financial support with Grant number 446630/2014-4 of funding of research no 014/2014 – Universal/MCT/CNPq and scholarship of postgraduate students. The authors thank FAPEAL and Apícola Fernão Velho for financial the support (TECNOVA) in research, development and innovation. The authors thank CNPq and FINEP for their financial support (CT-INFRA) in the acquisition of some equipment and laboratory facilities.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- De Mendonca IC, Porto IC, Do Nascimento TG, De Souza NS, Oliveira JM, Arruda RE, Mousinho KC, Dos Santos AF, Basilio-Junior ID, Parolia A et al (2015) Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells. BMC Complement Altern Med 15:357View ArticleGoogle Scholar
- Csermely P, Agoston V, Pongor S (2005) The efficiency of multi-target drugs: the network approach might help drug design. Trends Pharmacol Sci 26(4):178–182View ArticleGoogle Scholar
- Ozan F, Sümer Z, Polat ZA, Er K, Ozan U, Deger O (2007) Effect of mouthrinse containing propolis on oral microorganisms and human gingival fibroblasts. Eur J Dent 1:195–201Google Scholar
- Daugsch A, Fort P, Park YK (2008) Brazilian red propolis—chemical composition and botanical origin. Evid Based Complement Alternat Med 5:435–41View ArticleGoogle Scholar
- Grenho L, Barros J, Ferreira C, Santos VR, Monteiro FJ, Ferraz MP et al. In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite. Biomed Mater. 2015; doi:10.1088/1748-6041/10/2/025004
- Dota KF, Consolaro ME, Svidzinski TI, Bruschi ML. Antifungal activity of Brazilian propolis microparticles against yeasts isolated from vulvovaginal candidiasis. Evid Based Complement Alternat Med. 2011. doi:10.1093/ecam/neq029
- Pippi B, Lana AJ, Moraes RC, Güez CM, Machado M, de Oliveira LF et al (2015) In vitro evaluation of the acquisition of resistance, antifungal activity and synergism of Brazilian red propolis with antifungal drugs on candida spp. J Appl Microbiol 118:839–50View ArticleGoogle Scholar
- Nina N, Lima B, Feresin GE, Gimenez A, Salamanca Capusiri E, Schmeda-Hirschmann G (2016) Antibacterial and leishmanicidal activity of Bolivian propolis. Lett Appl Microbiol 62(3):290–296View ArticleGoogle Scholar
- Pontin K, da Silva FA, Santos F, Silva M, Cunha W, Nanayakkara NP, Bastos JK, Albuquerque S (2008) In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract. Parasitol Res 103:487–492. doi:10.1007/s00436-008-0970-z View ArticleGoogle Scholar
- Franchi Jr. GC, Moraes CS, Toreti VC, Daugsch A, Nowill AE, Park YK. Comparison of effects of the ethanolic extracts of Brazilian propolis on human leukemic cells as assessed with the MTT assay. Evid Based Complement Alternat Med. 2012; doi:10.1155/2012/918956
- Li F, Awale S, Tezuka Y, Kadota S (2008) Cytotoxic constituents from Brazilian red propolis and their structure-activity relationship. Bioorg Med Chem 15:5434–40View ArticleGoogle Scholar
- Begnini KR, de Leon PMM, Thurow H, Schultze E, Campos VF, Rodrigues FM, et al. Brazilian red propolis induces apoptosis-like cell death and decreases migration potential in bladder cancer cells. Evid Based Complement Alternat Med. 2014. doi:10.1155/2014/639856.
- De Mendonca IC, Porto IC, do Nascimento TG, de Souza NS, Oliveira JM, Arruda RE, Mousinho KC, dos Santos AF, Basilio-Junior ID, Parolia A et al (2015) Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells. BMC Complement Altern Med 15:357View ArticleGoogle Scholar
- Li F, Awale S, Tezuka Y, Kadota S (2008) Cytotoxic constituents from Brazilian red propolis and their structure-activity relationship. Bioorg Med Chem 16(10):5434–5440View ArticleGoogle Scholar
- Pinheiro KS, Ribeiro DR, Alves AV, Pereira-Filho RN, Oliveira CR, Lima SO, Reis FP, Cardoso JC, Albuquerque-Junior RL (2014) Modulatory activity of Brazilian red propolis on chemically induced dermal carcinogenesis. Acta Cir Bras 29(2):111–117View ArticleGoogle Scholar
- Begnini KR, de Leon PM M, Thurow H, Schultze E, Campos VF, Martins Rodrigues F, Borsuk S, Dellagostin OA, Savegnago L, Roesch-Ely M et al (2014) Brazilian red propolis induces apoptosis-like cell death and decreases migration potential in bladder cancer cells. Evid Based Complement Alternat Med 2014:639856View ArticleGoogle Scholar
- Olczyk P, Wisowski G, Komosinska-Vassev K, Stojko J, Klimek K, Olczyk M, Kozma EM. Propolis modifies collagen types I and III accumulation in the matrix of burnt tissue. Evid Based Complement Alternat Med 2013: 423809. doi: 10.1155/2013/423809
- Olczyk P, Komosinska-Vassev K, Winsz-Szczotka K, Stojko J, Klimek K, Kozma EM Propolis induces chondroitin/dermatan sulphate and hyaluronic acid accumulation in the skin of burned wound. Evid Based Complement Alternat Med 2013:290675. doi: 10.1155/2013/290675
- Silva BB, Rosalen PL, Cury JA, Ikegaki M, Souza VC, Esteves A et al (2008) Chemical composition and botanical origin of red propolis, a new type of Brazilian propolis. Evid Based Complement Alternat Med 5:313–6View ArticleGoogle Scholar
- Trusheva B, Popova M, Bankova V, Simova S, Marcucci MC, Miorin PL et al (2006) Bioactive constituents of Brazilian red propolis. Evid Based Complement Alternat Med 3:249–54View ArticleGoogle Scholar
- Hadinoto K, Sundaresan A, Cheow WS (2013) Lipid–polymer hybrid nanoparticles as a new generation therapeutic delivery platform: a review. Eur J Pharm Biopharm 85:427–443View ArticleGoogle Scholar
- Crauste-Manciet S, Larquet E, Khawand K, Bessodes M, Chabot GG, Brossard D, Mignet N (2013) Lipid spherulites: formulation optimisation by paired optical and cryoelectron microscopy. Eur J Pharm Biopharm 85:1088–1094View ArticleGoogle Scholar
- Sun X, Liu Z, Welsher K, Robinson J, Goodwin A, Zaric S et al (2008) Nano-graphene oxide for cellular imaging and drug delivery. Nano Res 1:203–212View ArticleGoogle Scholar
- Bzylinska U, Pucek A, Sowa M, Matczak-Jon E, Wilk KA (2014) Engeineering of phosphatidylcholine-based solid lipid nanocarriers for flavonoids delivery. Colloids and Surface A. Physicochem Eng Aspects 460:483–493View ArticleGoogle Scholar
- Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y (2009) Superparamagnetic grapheme oxide-Fe3O4 nanoparticles hybrid for controlled targeted drug carriers. J Matter Chem 19:2710–2714View ArticleGoogle Scholar
- Huang P, Xu C, Lin J, Wang C, Wang X, Zhang C et al (2011) Folic acid-conjugated grapheme oxide loaded with photosensitizers for targeting photodynamic therapy. Teranostics 1:240–250View ArticleGoogle Scholar
- Kaba SI, Egorova EM (2015) In vitro studies of the toxic effects of silver nanoparticles on HeLa and U937 cells. Nanotechnol, Sci Appl 8:19–29View ArticleGoogle Scholar
- Keck CM, Müller RH (2013) Nanotoxicological Classification System (NCS): a guide for risk-benefit assessment of nanoparticulate drug delivery system. Eur J Pharm Biopharm 84:445–448View ArticleGoogle Scholar
- Chowdhury SM, Lalwani G, Zhang K, Yang JY, Neville K, Sitharaman B (2013) Cell specific cytotoxicity and uptake of grahene nanoribbons. Biomaterials 34:282–293Google Scholar
- Bilati U, Allémann E, Doelker E (2005) Development of a nanoprecipitation method intended for the entrapment of hydrophilic drugs into nanoparticles. Eur J Pharm Sci 24:67–75View ArticleGoogle Scholar
- Abbas S, Hayat K, Karangwa E, Bashari M, Zhang X (2013) An overview of ultrasound-assisted food-grade nanoemulsions. Food Eng Rev 5:139–157View ArticleGoogle Scholar
- Chawla JS, Amiji MM (2002) Biodegradable poly(o-caprolactone) nanoparticles for tumor targeted delivery of tamoxifen. Int J Pharm 249:127–138View ArticleGoogle Scholar
- Ali M, Bahreini D, Shokri J, Samiei A, Kamali-Sarvestani E, Barzegar-Jalali M, Mohammadi-Samani S (2011) Nanovaccine for leishmaniasis: preparation of chitosan nanoparticles containing Leishmania superoxide dismutase and evaluation of its immunogenicity in BALB/c mice. Int J Nanomed 6:835–842Google Scholar
- Dhawan S, Kapil R, Singh B (2011) Formulation development and systematic optimization of solid lipid nanoparticles of quercetin for improved brain delivery. J Pharm Pharmacol 63(3):342–351View ArticleGoogle Scholar
- Kumari A, Yadav SK, Pakade YB, Singh B, Yadav SC (2010) Development of biodegradable nanoparticles for delivery of quercetin. Colloids Surf B Biointerfaces 80(2):184–192View ArticleGoogle Scholar
- Gatea F, Teodor ED, Seciu A-M, Covaci OI, Mănoiu S, Lazăr V, Radu GL (2015) Antitumour, antimicrobial and catalytic activity of gold nanoparticles synthesized by different ph propolis extracts. J Nanopart Res 17:320. doi:10.1007/s11051-015-3127-x View ArticleGoogle Scholar
- Luo H, Jiang B, Li B, Li Z, Jiang B-H, Chen YC (2012) Kaempferol nanoparticles achieve strong and selective inhibition of ovarian cancer cell viability. Int J Nanomedicine 7:3951–3959Google Scholar
- Qiu J-F, Gao X, Wang B-L, Wei X-W, Gou M-L, Men K, Liu X-Y, Guo G, Qian Z-Y, Huang M-J (2013) Preparation and characterization of monomethoxy poly(ethylene glycol)-poly(ε-caprolactone) micelles for the solubilization and in vivo delivery of luteolin. Int J Nanomed 8:3061–3069Google Scholar
- Song Q, Li D, Zhou Y, Yang J, Yang W, Zhou G, Wen J (2014) Enhanced uptake and transport of (+)-catechin and (−)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells. Int J Nanomed 9:2157–2165View ArticleGoogle Scholar
- Deshpande D, Devalapally H, Amiji M (2008) Enhancement in anti-proliferative effects of paclitaxel in aortic smooth muscle cells upon co-administration with ceramide using biodegradable polymeric nanoparticles. Pharmaceutal Res 25(8):1936–1947. doi:10.1007/s11095-008-9614-3 View ArticleGoogle Scholar
- Ahmad A, Syed F, Shah A, Zahid Khan Z, Kamran Tahir K, Khana AU, Yuan Q (2015) Silver and gold nanoparticles from Sargentodoxa cuneata: synthesis, characterization and antileishmanial activity. Royal Soc Chem Adv 5:73793–73806. doi:10.1039/c5ra13206a Google Scholar
- Ribeiro TG, Chávez-Fumagalli MA, Valadares DG, França JR, Rodrigues LB, Duarte MC, Lage PS, Andrade PHR, Lage DP, Arruda LV, Abánades DR, Costa LE, Martins VT, Tavares CAP, Castilho RO, Coelho EAF, Faraco AAG (2014) Novel targeting using nanoparticles: an approach to the development of an effective anti-leishmanial drug-delivery system. Int J Nanomed 9:877–890View ArticleGoogle Scholar
- Feng Z, Hao W, Lin X, Fan D, Zhou J (2014) Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice. OncoTargets Therapy 7:947–956Google Scholar
- Govender R, Phulukdaree A, Gengan RM, Anand K, Chuturgoon AA (2013) Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line. J Nanobiotechnol 11:5. doi:10.1186/1477-3155-11-5 View ArticleGoogle Scholar
- Khan M, Al-Marri AH, Khan M, Shaik MR, Mohri N, Adil SF, Kuniyil M, Alkhathlan HZ, Al-Warthan A, Tremel W, Tahir MN, Siddiqui MRH (2015) Green approach for the effective reduction of graphene oxide using salvadora persica l. root (miswak) extract. Nanoscale Res Lett 10:281. doi:10.1186/s11671-015-0987-z View ArticleGoogle Scholar
- Ke L-J, Gao G-Z, Shen Y, Zhou J-W, Rao P-F (2015) Encapsulation of aconitine in self-assembled licorice protein nanoparticles reduces the toxicity in vivo. Nanoscale Res Lett 10:449. doi:10.1186/s11671-015-1155-1 View ArticleGoogle Scholar
- Ibrahim MA, Khalaf AA, Galal MK, Ogaly HA, Hassan AHM (2015) Ameliorative influence of green tea extract on copper nanoparticle-induced hepatotoxicity in rats. Nanoscale Res Lett 10:363. doi:10.1186/s11671-015-1068-z View ArticleGoogle Scholar
- Bhattacharyya SS, Paul S, Khuda-Bukhsh AR (2010) Encapsulated plant extract (Gelsemium sempervirens) poly (lactide-co-glycolide) nanoparticles enhance cellular uptake and increase bioactivity in vitro. Exp Biol Med 235(6):678–688.Google Scholar
- Prakash DJ, Arulkumar S, Sabesan M (2010) Effect of nanohypericum (Hypericum perforatum gold nanoparticles) treatment on restraint stress induced behavioral and biochemical alteration in male albino mice. Pharmacognosy Res 2(6):330–334View ArticleGoogle Scholar
- da Silva SS, Thomé GS, Cataneo AHD, Miranda MM, Felipe I, Andrade CGTJ et al (2013) Brazilian propolis antileishmanial and immunomodulatory effects. Evid Based Complement Alternat Med 2013:673058. doi:10.1155/2013/673058 Google Scholar
- Lin Y-J, Liu Y-S, Yeh HH, Cheng T-L, Wang L-F (2012) Self-assembled poly(ε-caprolactone)-g-chondroitin sulfate copolymers as an intracellular doxorubicin delivery carrier against lung cancer cells. Int J Nanomed 7:4169–4183Google Scholar
- Ribeiro TG, Franca JR, Fuscaldi LL, Santos ML, Duarte MC, Lage PS, Martins VT, Costa LE, Fernandes SOA, Cardoso VN, Castilho RO, Soto M, Tavares CAP, Faraco AAG, Coelho EAF, Chávez-Fumagalli MA (2014) An optimized nanoparticle delivery system based on chitosan and chondroitin sulfate molecules reduces the toxicity of amphotericin B and is effective in treating tegumentary leishmaniasis. Int J Nanomed 9:5341–5353Google Scholar
- Mudavath SL, Talat M, Rai M, Srivastava ON, Sundar S (2014) Characterization and evaluation of amine-modified graphene amphotericin B for the treatment of visceral leishmaniasis: in vivo and in vitro studies. Drug Design, Development and Therapy 8: 1235–1247. http://dx.doi.org/10.2147/DDDT.S63994
- Valdez RH, Tonin LTD, Ueda-Nakamura T, Silva SO, Dias Filho BP, Kaneshima EM et al (2012) In vitro and In vivo trypanocidal synergistic activity of n-butyl-1-(4-dimethylamino)phenyl-1,2,3,4-tetrahydro-β-carboline-3-carboxamide associated with benznidazole. Antimicrob Agents Chemother 56:507–512. doi:10.1128/AAC.05575-11 View ArticleGoogle Scholar
- Huang F, You M, Chen T, Zhu G, Liang H, Tan W (2014) Self-assembled hybrid nanoparticles for targeted co-delivery of two drugs into cancer cells. Chem Commun 50:3103–3105View ArticleGoogle Scholar
- Croft SL, Sundar S, Fairlamb AH (2006) Drug resistance in leishmaniasis. Clin Microbiol Rev 1:111–126View ArticleGoogle Scholar
- Basselin M, Badet-Denisot MA, Lawrence F, Robert-Gero M (1997) Effects of pentamidine on polyamine level and biosynthesis in wild-type, pentamidine-treated, and pentamidine-resistante Leishmania. Exp Parasitol 85(3):275–282View ArticleGoogle Scholar
- Perez-Victoria FJ, Gamarro F, Ouellette M, Castanys S (2003) Functional clonig of the miltefosine transporter. A novel P-type phospholipid translocase from Leishmania involved in drug resistance. J Biol Chem 278(50):49965–49971View ArticleGoogle Scholar
- Turner KG, Vacchina P, Robles-Murguia M, Wadsworth M, McDowell MA, Morales MA (2015) Fitness and phenotypic characterization of miltefosine-resistant leishmania major. Plos Neglected Tropical Diseases 9(7):e0003948. doi:10.1371/journal.pntd.0003948
- Fessi H, Puisieux F, Devissaguet J-P, Ammoury N, Benita S (1989) Nanocapsule formation by interfacial polymer deposition following solvent displacement. Int J Pharm 55:R1–R4View ArticleGoogle Scholar
- Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss Technol 28:25–30View ArticleGoogle Scholar
- Brito MEF, Andrade MS, Mendonça MG, Silva CJ, Almeida EL, Lima BS, Félix SM, Abath FGC, Graça GC, Porrozzi R, Ishikawa EA, Shaw JJ, Cupolillo E, Brandão-Filho SP (2009) Species diversity of leishmania (Viannia) parasites circulating in an endemic area for cutaneous leishmaniasis located in the Atlantic rainforest region of northeastern Brazil. Trop Med Int Health 14(10):1278–1286View ArticleGoogle Scholar
- Machado GMC, Leon LL, De Castro SL (2007) Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo Cruz 102(1):73–77View ArticleGoogle Scholar
- Rocha LG, Aragão CFS, Loiola MIB, Bezerril RA, Paiva NRF, Holanda CMCX, Brito MEF (2009) Evaluation of the leishmanicide action of ethanol extracts of Crotalaria retusa L. (Fabaceae). Brazilian J Pharmacognosy 19(1A):51–56Google Scholar
- Quintanar-Guerrero D, Ganem-Quintanar A, Allémann E, Fessi H, Doelker E (1998) Influence of the stabilizer coating layer on the purification and freeze-drying of poly(D, L-lactic acid) nanoparticles prepared by an emulsion-diffusion technique. J Microencapsμlation 15:107–119View ArticleGoogle Scholar
- Raffin RP, Obach ES, Mezzalira G, Pohlmann AR, Guterres SS (2003) Nanocápsulas poliméricas secas contendo indometacina: estudo de formulação e tolerância gastrintestinal em ratos. Acta Farmacêutica Bonaerense 22(2):163–172Google Scholar
- Zili Z, Sfar S, Fessi H (2005) Preparation and characterization of poly-ε- caprolactone nanoparticles containing griseolfulvin. Int J Pharm 294:261–267View ArticleGoogle Scholar
- Scaffazick SR, Guterres SS, Lucca-Freitas L, Pohlamnn AR (2003) Caracterização e estabilidade físico-química de sistemas poliméricos nanoparticulados para administração de fármacos. Quim Nova 26:726–737View ArticleGoogle Scholar
- Nair HB, Sung B, Yadav VR, Kannappan R, Chaturvedi MM, Aggarwal BB (2010) Delivery of antiinflammatory nutraceuticals by nanoparticles for the prevention and treatment of cancer. Biochem Pharmacol 80(12):1833–1843View ArticleGoogle Scholar
- Duran N, Nuz M, Culha G, Duran G, Ozer B (2011) GC-MS Analysis and antileishmanial activities of two Turkish propolis types. Parasitol Res 108:95–105View ArticleGoogle Scholar
- Duran G, Duran N, Culha G, Ozcan B, Oztas H, Ozer B (2008) In vitro antileishmanial activity of Adana propolis samples on Leishmania tropica: a preliminary study. Parasitol Res 102:1217–1225View ArticleGoogle Scholar
- Ozbilge H, Kaya EG, Albayrak S, Silici S (2010) Anti- leishmanial activities of ethanolic extract of Kayseri propolis. Afr J Microbiol Resh 4(7):556–560Google Scholar
- Machado GMC, Leonor LL, De Castro SL (2007) Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo Cruz 102:73–77View ArticleGoogle Scholar
- Silva SS, Thomé GS, Cataneo AHD, Miranda MM, Felipe I, Andrade CGYJ, Watanabe MAE, Piana GM, Sforcin JM, Pavanelli WR, Conchon-Costa I. Brazilian propolis antileishmanial and immunomodulatory effects. Evidence-Based Complementary and Alternative Medicine. 2013; Article ID 673058: 7 pages. http://dx.doi.org/10.1155/2013/673058
- Miranda MM, Panis C, Cataneo AHD, Silva SS, Kawakami NY, Lopes LGF, Morey AT, Yamauchi LM, Andrade CGTJ, Cecchini R, Silva JJN, Sforcin JM, Conchon-Costa I, Pavanelli WR (2015) Nitric oxide and brazilian propolis combined accelerates tissue repair by modulating cell migration, cytokine production and collagen deposition in experimental leishmaniasis. PLoS One 10(5):14. doi:10.1371/journal.pone.0125101 Google Scholar
- Ayres DC, Marcucci MC, Giorgio S (2007) Effects of Brazilian propolis on Leishmania amazonensis. Mem Inst Oswaldo Cruz 102(2):215–220View ArticleGoogle Scholar
- Shaikh J, Ankola DD, Beniwal V, Singh D, Kumar MN (2009) Nanoparticle encapsulation improves oral bioavailability of curcumin by at least 9-fold when compared to curcumin administered with piperine as absorption enhancer. Eur J Pharm Sci 37:223–30View ArticleGoogle Scholar
- Zhou XJ, Hu XM, Yi YM, Wan J (2009) Preparation and body distribution of freeze-dried powder of ursolic acid phospholipid nanoparticles. Drug Dev Ind Pharm 35:305–10View ArticleGoogle Scholar
- Torres-Santos EC, Sampaio-Santos MI, Buckner FS, Yokoyama K, Gelb M, Urbina JA, Rossi-Bergmann B (2009) Altered sterol profile induced in Leishmania amazonensis by a natural dihydroxymethoxylated chalcone. J Antimicrobial Chemother 63:469–472View ArticleGoogle Scholar
- Brajtburg J, Bolard J (1996) Carrier effects on biological activity of amphotericin B. Clin Microbiol Rev 9:512–531Google Scholar
- Chen M, Zhai L, Christensen SB, Theander TG, Kharazmi A (2001) Inhibition of fumarate reductase in leishmania major and l. donovani by chalcones. Antimicrobial Agents Chemother 45(7):2023–2029View ArticleGoogle Scholar
- Maarouf M, Lawrence F, Croft SL, RobertGero M (1995) Ribosomes of Leishmania are a target for the aminoglycosides. Parasitol Res 81:421–425View ArticleGoogle Scholar
- Maarouf M, Kouchkovsky Y, Brown S, Petit PX, RobertGero M (1997) In vivo interference of paramomycin with mitochondrial activity of Leishmania. Exp Cell Res 232:339–348View ArticleGoogle Scholar