Inhibition of HIV Virus by Neutralizing Vhh Attached to Dual Functional Liposomes Encapsulating Dapivirine

Table 1 Characterisation of the immunoliposomes

Liposome	PC conc. (mg/ml)^a	Dapivirine (μM)	No. of Vhh molecules per liposome	Vhh (μg/ml)	Diameter (nm)^a	Zeta potential (mV)^a
Lipo1-lab5	11.937 ± 0.001	–	160	126.3	228.0 ± 1.0	−45.0 ± 0.9
Lipo1-J3	11.708 ± 0.008	–	160	118.4	223.4 ± 4.5	−37.4 ± 0.5
Lipo2-lab5	5.974 ± 0.005	–	95	150.0	130.9 ± 1.4	−38.7 ± 0.7
Lipo2-J3	6.149 ± 0.003	–	108	159.9	131.2 ± 1.9	−38.5 ± 0.2
Lipo2-dapi	6.478 ± 0.001	6.5	–	–	122.9 ± 1.8	−46.0 ± 0.9
Lipo2-dapi-J3	5.548 ± 0.002	5.5	125	169.6	123.4 ± 1.8	−38.5 ± 0.4
Lipo3 control	7.573 ± 0.003	–	–	–	130.9 ± 1.7	−42.5 ± 0.4
Lipo3-J3	6.456 ± 0.004	–	121	168.2	142.2 ± 3.2	−36.4 ± 0.4
Lipo3-dapi	6.675 ± 0.003	6.7	–	–	128.2 ± 2.6	−41.7 ± 0.5
Lipo3-dapi-J3	6.806 ± 0.001	6.8	108	157.0	139.1 ± 1.8	−37.8 ± 0.6

Dapi: dapivirine
^aData are means ± standard deviations of three replicate measurements in one representative experiment of three independent experiments