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Recent Advances in Silicon Nanowire Biosensors: Synthesis Methods, Properties, and Applications
Nanoscale Research Lettersvolume 11, Article number: 406 (2016)
The application of silicon nanowire (SiNW) biosensor as a subtle, label-free, and electrical tool has been extensively demonstrated by several researchers over the past few decades. Human ability to delicately fabricate and control its chemical configuration, morphology, and arrangement either separately or in combination with other materials as lead to the development of a nanomaterial with specific and efficient electronic and catalytic properties useful in the fields of biological sciences and renewable energy. This review illuminates on the various synthetic methods of SiNW, with its optical and electrical properties that make them one of the most applicable nanomaterials in the field of biomolecule sensing, photoelectrochemical conversion, and diseases diagnostics.
Silicon nanowire (SiNW) biosensors are typical field effect transistor (FET)-based devices, made up of three electrodes. The mechanism of their sensing process is due to the variation in their charge density that leads to changes in the electric field at the external surface of the SiNW. Practically speaking, the resistivity of the device is increased when a negatively charged biomolecules species is synthesized with the external surface of an n-type SiNW. Furthermore, rare properties like great surface-to-volume ratio, tunable electrical and optical properties, and biocompatibility possessed by SiNW have made them good candidates for the detection of metal ions species, nucleic acids, and virus (Table 1).
The three electrodes making up a SiNW consist of a source and drain an electrode that connects the semiconductor channel together and the third electrode; gate electrode regulates and maintains the conductance of the channel. It should be noted that the ability of this device to sense is as a result of the location of SiNW between the source electrode and the drain electrode in the semiconductor channel (Table 2).
SiNWs Synthesis Techniques
Generally, there are presently two procedures that have been developed for the nanofabrication processes of SiNWs, and they include top–down approach (Fig. 4) and bottom–up approach (Fig. 5). The efficient performance of the SiNW biosensor can be determined by various factors like diameters, carrier densities, and surface chemistry. An in-depth discussion about the bottom–up of the synthesis of SiNWs has been reported by Ramanujam et al. . The bottom–up approach includes processes like vapor-liquid-solid (VLS) and oxide-assisted growth (OAG) and photolithography or e-beam lithography . VLS technique has been reported to adopt to synthesize SiNWs, along with their applications as biosensors. The bottom–up method involves the synthesis of the SiNWs from a mass of silicon wafer with the reaction been metal catalyzed, while top–down technique begins from a bulk silicon wafer and trims down to the preferred and required size and shape of SiNWs through a lithographic mechanism. For comparison, see Tables 3 and 4.
Silicon nanowire synthesis via VLS was first reported in 1964 using silicon substrate integrated with liquid Au droplet. In VLS, there is a deposition of metal-catalyzed (Au, Fe, Pt, Al, etc.) on the silicon wafer and then the SiNWs growth is augmented either by chemical vapor deposition (CVD) technique [3, 4] (Fig. 1). Essentially, silicon wafer coated with metal catalysts are positioned at the middle of a tube furnace and initiated with a silane (SiH4) or tetrachlorosilane (SiCl4) and passed above the metal catalyst accumulated on Si wafer in the chamber at above eutectic temperature .
The SiH4 gas serving as the source of silicon gas would be converted into silicon vapor and disperses through a metal catalyst to produce metal-silicon alloy droplets. As silicon diffuses across the metal nanoparticle catalyst leading to a supersaturated state of condition, the silicon will precipitate out from droplets of metal-Si forming silicon nanowires .
OAG via Thermal Evaporation
Recently, many researchers have effectively synthesized SiNWs via a bottom–up approach called OAG via thermal evaporation due to its in generating a huge quantity of SiNWs . Using OAG method, the growth of SiNWs was significantly improved using SiO as starting material to stimulate the nucleation and the growth of SiNWs without the use of catalyzed metal generating high purities SiNWs and free of metal impurities . The development of SiNWs using OAG method has been reported by Shao et al. . Briefly, they reported that the alumina boat holding the mixture of SiO powder (10 g) and Si powder (0.05 g) was positioned at the alumina tube, inside a tube furnace. At particular pressure, Argon was introduced as a carrier gas and for 10 h, the furnace was heated to a temperature of 1250–1300 °C. The resulting SiNWs are with a diameter of 85 nm and were gathered around the alumina tube surface (Fig. 2). One of the features of the produced SiNWs via OAG method is it possesses at its outer layer, an oxide layer that is chemically inert. To efficiently improve the electrical and optical properties of the produced SiNWs, the outer layer covered by oxide layer should be removed by treating the oxide layer with hydrofluoric acid (HF).
It should be noted that this method is more preferable to VLS as it enables to produce SiNWs with various morphologies in chains, rods, wires, ribbons, and coaxial structures, and the use of silicon sources like silane (SiH4) or SiCl4 can be circumvented.
Metal-Assisted Chemical Etching
This is the most low-cost and simple method of synthesizing SiNMs . This method comprises two main stages which are electroless metal (silver, nickel, platinum, gold) deposition on silicon wafer followed by chemical etching in fluoride-ion-based solution [11, 12]. The real-time reaction of electro-less deposition and chemical etching has been reported by Brahiti and co-workers , and it entails soaking of cleaned silicon wafer into NH4HF2 and AgNO3 solution.
In this method, silver ion attracts electrons from the silicon substrate that stemmed from the deposition of silver nanoparticle on silicon surface . The silicon underneath the silver nanoparticle is oxidized and holes are formed by the action of HF; the holes formed serve as a sinking route for the residual of the Ag nanoparticles thereby forming a longitudinal and lateral suspension of silicon generating the formation of SiNWs arrangements  (Fig. 3). Zhang and co-workers  also reported that when parameters like temperature, concentration and deposition time, and doping level are manipulated, diverse morphologies of SiNWs arrays could be produced.
Alignment OF SiNWs
The bottom–up approach for the synthesis of SiNWs is appealing as it leads to the production of efficient high-quality, minute diameter of about 3–5 nm and single-crystalline SiNWs. However, it should be noted that in order to utilize SiNWs in the fabrication of any device, SiNWs need assemblage with the coordinated transfer, alignment, and density on a device substrate for successive incorporation with the circuitry in a spatially defined approach.
Various methods have been reported in several kinds of literature that permit well-ordered configuration and assembly of one-dimensional SiNWs into a required design. These methods include Langmuir–Blodgett (LB), blown–bubble (BB), microfluidic flow, electric field, and contact printing alignment. In brief, the details of these methods are described in Table 2 below.
Presently, there are two approaches to fabricate SiNW devices, namely top–down and bottom–up . Several researchers have reported in details the modalities behind top–down approaches for fabrication of SiNW [23, 26]. Table 2 below shows some differences between the two fabrication approaches as regards the merit and demerit related with each fabrication techniques. The top–down approach involves the synthesis of SiNWs starting from the bulk material and scaled down into a distinct SiNW that can be produced via the process of nanolithography techniques like electron beam lithography (EBL)  and nanoimprint lithography and so on . The synthesis via top–down technique has been reported by Park and co-workers  by using electron beam lithography and reactive ion etching on silicon-on-insulator (SOI) wafer leading to the production of high-pitched control of the geometry and alignment of SiNWs with efficient electrical properties. In addition, Vu and colleagues demonstrated SiNW arrangement with width dimensions of 20-nm width and 60-nm height  which possess the features of both nanoimprint lithography and wet anisotropic etching. Furthermore, the use of DEA technology and photolithography technique has been reported by a group of researchers to produce a lone SiNW with radius below 50 nm and 1 mm in height  (Fig. 4).
In another recent work by Kulkarni and co-workers, they were able to efficiently fabricate SiNW arrays of about 250 nanowires with dimensions of 150 nm breadth and 20 μm in length with 3.2 nm similar space size via top–down approach . It should be noted that they adopted the four stages of photolithography techniques in their research (Fig. 5).
Properties of Silicon Nanowires
The electronic and electrical properties of SiNW greatly and strongly depends on growth direction, size, morphology, and surface reconstruction because of their small sizes which are so evident in the size dependence of the electronic band gap width of SiNWs regardless of wire direction. The diameter of the wire is inversely proportional to the width of the band gap resulting into a deviation from the bulk silicon. In addition, the alignment of the wire axis and its surface area has some effects on the electronic properties of SiNWs.
Michael Nolan and co-workers investigated the band gap modification for small diameter of about 0.9–1 nm silicon nanowires fabricated by the use of several types of surface termination by density functional theory calculations (Fig. 6). The 0.9–1-mm nanowire demonstrated a direct band gap that increases concomitantly with a decrease in the diameter of the wire because of quantum limitation, regardless of surface termination.
Furthermore, Sacconi and co-worker also demonstrated the electronic properties of silicon nanowires with varying approaches such as Empirical Tight-Binding (ETB) model, the Linear Combination of Bulk Bands (LCBB) model, and Non-Equilibrium Green Function (NEGF) model by investigating both hydrogenated and SiO2 terminated silicon surfaces in these models.
The diameter of SiNW reduced from 3.2 to 1.6 nm concomitantly with an increase in the band gap of hydrogenated nanowire from 1.56 to 2.44 eV. However, Sacconi reported a minute increase in the SiO2/SiNW structure. This phenomenal is as a result of lower restriction caused by SiO2 trapping the SiNW when compared to simple hydrogen termination. They also reported effective masses for conduction and valence bands. Reduction of the conduction mass, from 0.47 mo to 0.31 m is equal to the effect of increasing the thickness of silicon on a hydrogen-terminated wire but the effect on the SiO2-confined wire was the same as a result of increase in silicon thickness and a decrease in effective mass from 0.36 to 0.29 mo .
Silicon bulk possesses an indirect band gap coupled with the valence band maximum at the Γ point and the conduction minimum at about 85 % along the Γ to X direction, and a phonon is needed to sustain the momentum in any electronic transition. Outstandingly, SiNWs developed laterally and most of the crystallographic orientations have a direct band gap; as a result, both the maximum and minimum of the valence band and the conduction band respectively occur at a similar point in k-space. This unique property has made SiNWs as effective optically active materials for photonics applications. Controlling the band gap width can open new doors to the application of SiNWs in optoelectronics fields: such that both the band gap and width of SiNWs can be tuned to increase its optical efficiency. The possibility of tuning the band gap and width of SiNWs is determined by controlling the chemical composition and the coverage density of the wire surface area, and it has been regarded as an easier and effective route for tuning. Leu and co-workers reported that chlorine, bromine, and iodine can be used in place of hydrogen as a surface passivation agents because they have the ability to reduce the band gap but still maintaining the semiconducting abilities of the wires .
Recently, Ramos and colleagues demonstrated the optical and mechanical characterization of SiNWs by showing experimental and theoretical data to investigate the fundamental mechanisms behind the light-nanowire interaction in an optical interferometry setup .
In the experiment, they synthesized silicon nanowires horizontally compiled and epitaxially clamped at the sidewalls of pre-patterned micro-trenches on Si substrates via the vapor-liquid-solid approach . The length and diameter of the fabricated nanowires were between 8 and 16 μm and 40 and 240 nm, respectively, and a vertical distance between 1.0 and 1.3 μm was between the nanowire and the substrate underneath. One of the most important features of this study was the selection of growth conditions to synthesize tapered nanowires in which the diameter linearly decreases from the clamped end to the free end . Optical interferometer at room temperature was used to measure the mechanical vibration of the nanowires  in a Fabry-Perot configuration operating at a wavelength of 633 nm. Figure 7a depicts a scanning electron microscopy (SEM) diagram of one of the fabricated tapered nanowires and a frequency spectrum of the thermo-mechanical oscillations that displays the quasi-degeneration of the two orthogonal fundamental vibration modes . The fabricated tapered nanowire has a length of 11.3 μm, and there was a reduction in the diameter from 150 ± 5 nm at the clamp area to 60 ± 5 nm at the tip as determined by the electron microscopy.
The tapered nanowire displayed colors ranging throughout the visible spectrum between the clamped and loose ends when it is observed under a dark-field microscopy (Fig. 7b) [48, 49]. Given that the dark-field microscopy has the optimum capability to detect only scattered light, as such the emitted colors of the collected light solely comes from the light scattered by the tapered nanowire. In addition, Ramos et al. reported that numerical simulations from their studies of light-silicon nanowire interaction demonstrated that silicon nanowires display optical resonances that competently improve the light scattering for a specific wavelength values to diameter ratio. These optical resonances created a connection between the diameter of the tapered nanowire and the scattered light’s color similar to that of the dark-field data .
Ramos et al. in order to investigate more on the optical resonances of the tapered nanowire, at a wavelength of 633 nm, the scattering efficiency of the nanowire was calculated as a function of its diameter that is depicted in Fig. 7b for transverse magnetic (TM) and transverse electric (TE) azimuthal polarizations . The spectra illustrated a series of optical resonances indicating the location of strong scattering of light while the light limitation within the nanowire is been demonstrated by the spatial distribution of the near electric-field intensity at these resonances (Fig. 7b). It should be noted that there is a generation of evanescent field resulting from the electromagnetic field extending some few nanometers away from the nanowire because of the small size of the nanowire and thus the resonances can proficiently relate with the neighboring electromagnetic field .
Application of SiNWs
SiNWs as Ion-Selective Nanosensors
Cui and co-workers reported the first case of SiNW application as chemical transducers in 2001  (Tables 5 and 6). Cui and colleagues produced a pH nanosensor by modifying p-type (boron-doped) SiNWs with an APTES film. Depending on the pH of the solution, the APTES film was subjected to the process of protonation or de-protonation, which regulated the surface charge on the SiNWs and gated the conductance of the NWs in a pH-dependent manner (Fig. 8ii).
It can be seen that the SiNW conductance increased concomitantly with an increase in pH from 2 to 9 in a linear manner, and at a specific pH value, the conductance of the SiNW was constant. Figure 11a(i) shows the surface modification of SiNW with APTES with an introduction of primary amine functional groups to the underlying surface silanol groups that resulted into the protonation of NH2 group into NH3 + at low pH (Fig. 8i). The density of the charge carriers in the p-type SiNW was depleted as a result of the positive surface charge thereby leading to a reduction in conductance. At an alkaline pH, the surface silanol groups were deprotonated to SiO (Fig. 8iii), leading to a concomitant accumulation of charge carriers in the p-type SiNW and increase in conductance. However, conductance measurements carried on unmodified SiNWs demonstrated a non-linear dependence on changes of pH (Fig. 8iv).
Recently, Chen and co-workers also reported that the functional group present on the surface of a SiNW was responsible for the pH sensitivity of the SiNW-FET sensor . Furthermore, it has also been reported that Dorvel et al. produced SiNW-FETs via top–down method with hafnium oxide (HfO2)-based gate dielectric interfaces for pH sensing that gave a response of ca. 56 mV/pH . However, there is a possibility that the pH sensitivity of NW-FET sensors can exceed the Nernst limit by operating the device under dual gate  or in a DG configuration .
Nucleic Acid and DNA Detection Using SiNWs
Nucleic acids have been reported to be labeled and successfully detected by SiNW-FETs thereby making them attractive sensors. The negative charge related to the sugar-phosphate backbone of DNA and RNA allows sensitive detection of nucleic acids with detection limits in the fM range [34, 54] (Table 5). DNA probes that are electrostatically neutral can be used to attain comparative changes in surface charge and this is evident in the use of PNA  and alkyl-phosphonate oligonucleotide  chemistries in probe production that lead to an enhanced signal-to-noise ratio as compared to DNA. In addition, Jiang and co-workers  have produced a SiNW integrated with AgNPs through metal-assisted chemical etching method-based sandwich structural DNA SERS sensor for multiplex DNA detection. Jiang and colleagues reported that immobilization of thiolated single-stranded DNA probe functionalized with silver nanoparticles through Ag-S bonding and hybridization with the target reporter probe marked with Rhodamine 6G before SERS detection was done (Fig. 9). This significant approach demonstrated high reproducibility and specifically for DNA detection coupled with the fact that SERS sensor is efficient of distinguishing single base mismatched DNA at lower concentrations of 1 pM.
Han et al.  reported the optimized single SiNWs-AgNPs for surface-enhanced Raman scattering detection of pesticide residues (carbaryl) on the surface of a cucumber in regard to merits like a rapid response, easiness, elasticity, and increased resolution. Han and colleagues also demonstrated the discovery of Escherichia coli-based SERS sensor by filtering the AgNPs-SiNWs because the water has been contaminated with E. coli and then followed by characterization by Raman spectroscopy (Fig. 10a, b).
Fluorescence’s Sensor-Utilized SiNWs
Recently, Su and co-workers  demonstrated a novel AuNP-SiNW-based molecular beacons (MBs) for high-sensitivity multiplex DNA detection (Figs. 11 and 12). They reported that AuNP-SiNW-based MBs displayed stout stability in wide salt concentrations within the range of 0.01–0.1 M and thermal stability within 10–80 °C. And in addition, it slowly accumulated as a result of the salt-induced reduction of electrostatic between AuNPs at an increased concentration of salt . Su and co-workers reported that after the process of DNA hybridization, there were conformational changes in the stem loop of MBs leading to spatial separation of the carboxyfluorescein and AuNPs-SiNWs, thus improving the fluorescence intensity.
Finally, Su discovered that the fluorescence intensity was significantly augmented with an increased concentration of target DNA from 50 pM to 10 nM, Conclusively, the authors reported that AuNPs-SiNWs based on MBs were efficient in detecting DNA target at reduced concentrations down to pM level and also exhibited high selectivity in the presence of non-complementary DNA and single base mismatch.
Furthermore, Han recently demonstrated another application of SiNWs  for fluorescence protein immunosensor development. They reported the construction of vertically aligned SiNW arrays with a dimension of 8 μm in height and 75 μM in radius through electro-less etching (AEE) process, and protein were covalently trapped onto APTES-modified SiNWs.
As a result of high aspect ratio of SiNW-produced high surface of SiNWs that increased the immobilization of loaded BSA protein, based on this potent positive result of BSA immobilization using modified SiNWs-BSA, Han and colleagues were impressed to fabricate two types of immunosensor assays between IgG and FITC-anti-Ig-G (fluorescein isocyanate) and IgM and Cys3-anti IgM. In conclusion, they reported in their findings that fluorescence intensity due to the bond between anti-IgG and anti-IgM was greatly enhanced using SiNWs compared with planar substrates (Figs. 13 and 14).
FET Sensor-Utilized SiNWs
SiNW sensors are classic FET-based devices, composed of three electrodes. The variation in charge density can shed more light on the mechanism of the sensing process, which stimulates a change in the electric field at the SiNW outer surface. In practical, a negatively charged biomolecules species integrated to the outer surface of an n-type SiNW increases the resistivity of the device and vice versa if using p-type SiNWs . More recently, Gao et al.  have fabricated a high performance of label-free and direct time for DNA detection using SiNWs-FET sensor via top–down approach. In their research work, they efficiently improved the sensitivity of the SiNWs-FET sensor by optimization of qualities like gate voltage, probe concentration, and buffer ionic strength. In brief, SiNW surface was firstly customized by the amine group of APTES and functionalized with carboxyl (COOH–) group modified target DNA via N-hydroxysuccinimide (NHS) and 1-ethyl 3-(3-dimethylaminopropyl) carbodiimide (EDC). Conclusively, Gao and co-workers reported that the enhanced SiNWs-FET sensor demonstrated a detection limit of 0.1 fM for DNA target (Fig. 15). In addition, the existing change presented around 40 % when DNA probe hybridized with full complementary target DNA and presented with only 20 and 5 % upon the introduction of single and second base mismatched DNA. It should also be noted that Zhang et al.  have investigated for the very first time the development of SiNWs-FET sensor based on the carbohydrate-protein interaction where unmodified carbohydrate is immobilized through the formation of an oxime bonding. Zhang and colleagues’ investigations on the newly fabricated sensor demonstrated increased specificity of lectin EC detection via galactose-modified SiNW sensor which is able to detect as low as 100 fg/m, as against 400 fg/m of other previously investigated sensors (Fig. 16).
Several research works have investigated the efficiency of SiNWs and SiNW coupled with metal nanoparticles like gold and silver nanoparticles and have labeled it as excellent sensing material electrodes with high-quality catalytic activity and conductivity that can be harnessed in different fields due to their unique characterization (high detection, portability, and easiness of the procedure [77–79]. However, there are still few restraints to overcome [80–82].
Firstly, the two main broad fabrication techniques of SiNWs must be more efficiently developed to guarantee the dependable electrochemical and electrical SiNW sensor . In addition, parameter manipulations in SiNW synthesis in terms of its alignment, surface area, and diameters are to be done in other to fabricate a highly controlled and reproducible sensor-based SiNWs .
Secondly, via the bottom–up techniques, the produced SiNW lacks control, accurate alignment, and identical precise direction as such new and improved synthesis technique is needed with greater control and accurate alignment [85–87]. Although this problem has been solved in top–down approach but its expensive cost of SiNW fabrication sensors still poses a problem for several manufacturers. Conclusively, SiNW is the promising nanomaterial sensing in the nearest future.
Ramanujam J, Shiri D, Verma A (2011) Silicon nanowire growth and properties: a review. Mater Express 1:105–126
Chen KI, Li BR, Chen YT (2011) Silicon nanowire field-effect transistor-based biosensors for biomedical diagnosis and cellular recording investigation. Nano Today 6:131–154
Jamal IP, Chong SK, Chan KW, Othman M, Abdul Rahman S, Aspanut Z (2013) Formation of silicon/carbon core-shell nanowires using carbon nitride nanorods template and gold catalyst. J Nanomater 7:1–7.
Hong TWH, FCN (2012) A novel method to grow vertically aligned silicon nanowires on Si (111) and their optical absorption. J Nanomater 2012:9
Schmidt V, Wittemann JV, Senz S, Gösele U (2009) Silicon nanowires: a review on aspects of their growth and their electrical properties. Adv Mater 21:2681–2702
Bandaru PR, Pichanusakorn P (2010) An outline of the synthesis and properties of silicon nanowires. Semicond Sci Technol 25:024003
Suzuki H, Araki H, Tosa M, Noda T (2007) Formation of silicon nanowires by CVD using gold catalysts at low temperatures. Mater Trans 48:2202–2206
Yang L, Lin H, Zhang Z, Cheng L, Ye S, Shao M (2013) Gas sensing of tellurium-modified silicon nanowires to ammonia and propylamine. Sensors Actuators B Chem 177:260–264
Shao M-W, Zhang N-BW M-L et al (2008) Ag-modified silicon nanowires substrate for ultrasensitive surface-enhanced raman spectroscopy. Appl Phys Lett 93:233118
Huang Z, Geyer N, Werner P, De Boor J, Gösele U (2011) Metal-assisted chemical etching of silicon: a review. Adv Mater 23:285–308
Bai F, Li M, Song D, Yu H, Jiang B, Li Y (2012) One-step synthesis of lightly doped porous silicon nanowires in HF/AgNO3/H2O2 solution at room temperature. J Solid State Chem 196:596–600
Kolasinski KW (2005) Silicon nanostructures from electroless electrochemical etching. Curr Opin Solid State Mater Sci 9:73–83
Brahiti N, Bouanik S-A, Hadjersi T (2012) Metal-assisted electroless etching of silicon in aqueous NH4HF2 solution. Appl Surf Sci 258:5628–5637
Megouda N, Douani R, Hadjersi T, Boukherroub R (2009) Formation of aligned silicon nanowire on silicon by electroless etching in HF solution. J Lumin 129:1750–1753
Shiu SC, Lin SB, Hung SC, Lin CF (2011) Influence of pre-surface treatment on the morphology of silicon nanowires fabricated by metal-assisted etching. Appl Surf Sci 257:1829–1834
Zhang ML, Peng KQ, Fan X, Jie JS, Zhang RQ, Lee ST, Wong NB (2008) Preparation of large-area uniform silicon nanowires arrays through metal-assisted chemical etching. J Phys Chem C 112:4444–4450
He B, Morrow TJ, Keating CD (2008) Nanowire sensors for multiplexed detection of biomolecules. Curr Opin Chem Biol 12:522–528
Yu G, Cao A, Lieber CM (2007) Large-area blown bubble films of aligned nanowires and carbon nanotubes. Nat Nanotechnol 2:372–377
Huang Y, Duan X, Wei Q, Lieber CM (2001) Directed assembly of one-dimensional nanostructures into functional networks. Science 291:630–633
Freer EM, Grachev O, Duan X, Martin S, Stumbo DP (2010) High-yield self-limiting single-nanowire assembly with dielectrophoresis. Nat Nanotechnol 5:525–530
Xu F, Durham JW, Wiley BJ, Zhu Y (2011) Strain-release assembly of nanowires on stretchable substrates. ACS Nano 5:1556–1563
Durham JW, Zhu Y (2013) Fabrication of functional nanowire devices on unconventional substrates using strain-release assembly. ACS Appl Mater Interfaces 5:256–261
Penner RM (2012) Chemical sensing with nanowires. Annu Rev Anal Chem 5:461–485
Reddy B, Dorvel BR, Go J et al (2011) High-k dielectric Al2O3 nanowire and nanoplate field effect sensors for improved pH sensing. Biomed Microdevices 13:335–344
Dorvel BR, Reddy B, Go J, Duarte Guevara C, Salm E, Alam MA, Bashir R (2012) Silicon nanowires with high-k hafnium oxide dielectrics for sensitive detection of small nucleic acid oligomers. ACS Nano 6:6150–6164
Hobbs RG, Petkov N, Holmes JD (2012) Semiconductor nanowire fabrication by bottom-up and top-down paradigms. Chem Mater 24:1975–1991
Yang L, Lin H, Wang T, Ye S, Shao M (2012) Tellurium-modified silicon nanowires with a large negative temperature coefficient of resistance. Appl Phys Lett 101:133111
Park I, Li Z, Pisano AP, Williams RS (2010) Top-down fabricated silicon nanowire sensors for real-time chemical detection. Nanotechnology 21:015501
Vu XT, GhoshMoulick R, Eschermann JF, Stockmann R, Offenhäusser A, Ingebrandt S (2010) Fabrication and application of silicon nanowire transistor arrays for biomolecular detection. Sensors Actuators B Chem 144:354–360
Pham VB, Pham XTT, Dang NTD, Le TTT, Tran PD, Nguyen TC, Nguyen VQ, Dang MC, van Rijn CJM, Tong DH (2011) Detection of DNA of genetically modified maize by a silicon nanowire field-effect transistor. Adv Nat Sci Nanosci Nanotechnol 2:25010
Kulkarni A, Xu Y, Ahn C, Amin R, Park SH, Kim T, Lee M (2012) The label free DNA sensor using a silicon nanowire array. J Biotechnol 160:91–96
Tong HD, Chen S, Van Der Wiel WG, Carlen ET, Den Van Berg A (2009) Novel top-down wafer-scale fabrication of single crystal silicon nanowires. Nano Lett 9:1015–1022
Jiang Z, Qing Q, Xie P, Gao R, Lieber CM (2012) Kinked p-n junction nanowire probes for high spatial resolution sensing and intracellular recording. Nano Lett 12:1711–1716
Gao A, Lu N, Wang Y, Dai P, Li T, Gao X, Wang Y, Fan C (2012) Enhanced sensing of nucleic acids with silicon nanowire field effect transistor biosensors. Nano Lett 12:5262–5268
Masood MN, Chen S, Carlen ET, Van Den Berg A (2010) All-(111) surface silicon nanowires: Selective functionalization for biosensing applications. ACS Appl Mater Interfaces 2:3422–3428
Ahn JH, Kim JY, Seol ML, Baek DJ, Guo Z, Kim CH, Choi SJ, Choi YK (2013) A pH sensor with a double-gate silicon nanowire field-effect transistor. Appl Phys Lett 102:083701. doi:10.1063/1.4793655
Ahn JH, Choi SJ, Han JW, Park TJ, Lee SY, Choi YK (2010) Double-gate nanowire field effect transistor for a biosensor. Nano Lett 10:2934–2938
Qing Q, Pal SK, Tian B, Duan X, Timko BP, Cohen-Karni T, Murthy VN, Lieber CM (2010) Nanowire transistor arrays for mapping neural circuits in acute brain slices. Proc Natl Acad Sci U S A 107:1882–1887
Hu J, Odom TW, Lieber CM (1999) Chemistry and physics in one dimension: Synthesis and properties of nanowires and nanotubes. Acc Chem Res 32:435–445
Gao Z, Agarwal A, Trigg AD, Singh N, Fang C, Tung CH, Fan Y, Buddharaju KD, Kong J (2007) Silicon nanowire arrays for label-free detection of DNA. Anal Chem 79:3291–3297
Nolan M, O’Callaghan S, Fagas G, Greer JC, Frauenheim T (2007) Silicon nanowire band gap modification. Nano Lett 7:34–38
Sacconi F, Persson MP, Povolotskyi M, Latessa L, Pecchia A, Gagliardi A, Balint A, Fraunheim T, Di Carlo A (2007) Electronic and transport properties of silicon nanowires. J Comput Electron 6:329–333
Leu PW, Shan B, Cho KJ (2006) Surface chemical control of the electronic structure of silicon nanowires: density functional calculations. Phys Rev B 73:195320
Ramos D, Gil-Santos E, Malvar O, Llorens JM, Pini V, Paulo AS, Calleja M, Tamayo J (2013) Silicon nanowires: where mechanics and optics meet at the nanoscale. Sci Rep 3:3445
Ramos D, Gil-Santos E, Pini V, Llorens JM, Fernández-Regúlez M, San Paulo Á, Calleja M, Tamayo J (2012) Optomechanics with silicon nanowires by harnessing confined electromagnetic modes. Nano Lett 12:932–937
Malvar O, Gil-Santos E, Ruz JJ, Ramos D, Pini V, Fernandez-Regulez M, Calleja M, Tamayo J, San Paulo A (2013) Tapered silicon nanowires for enhanced nanomechanical sensing. Appl Phys Lett. doi: 10.1063/1.4813819
Gil-Santos E, Ramos D, Martínez J, Fernández-Regúlez M, García R, San Paulo A, Calleja M, Tamayo J (2010) Nanomechanical mass sensing and stiffness spectrometry based on two-dimensional vibrations of resonant nanowires. Nat Nanotechnol 5:641–645
Seo K, Wober M, Steinvurzel P, Schonbrun E, Dan Y, Ellenbogen T, Crozier KB (2011) Multicolored vertical silicon nanowires. Nano Lett 11:1851–1856
Brönstrup G, Jahr N, Leiterer C, Csäki A, Fritzsche W, Christiansen S (2010) Optical properties of individual silicon nanowires for photonic devices. ACS Nano 4:7113–7122
Cao L, White JS, Park J-S, Schuller JA, Clemens BM, Brongersma ML (2009) Engineering light absorption in semiconductor nanowire devices. Nat Mater 8:643–647
Cui Y, Wei Q, Park H, Lieber CM (2001) Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science 293:1289–1292
Chen S, Bomer JG, Carlen ET, Van Den Berg A (2011) Al2O3/silicon nanoISFET with near ideal nernstian response. Nano Lett 11:2334–2341
Knopfmacher O, Tarasov A, Fu W, Wipf M, Niesen B, Calame M, Schönenberger C (2010) Nernst limit in dual-gated Si-nanowire FET sensors. Nano Lett 10:2268–2274
Chu CJ, Yeh CS, Liao CK, Tsai LC, Huang CM, Lin HY, Shyue JJ, Chen YT, Chen CD (2013) Improving nanowire sensing capability by electrical field alignment of surface probing molecules. Nano Lett 13:2564–2569
Zhang GJ, Chua JH, Chee RE, Agarwal A, Wong SM (2009) Label-free direct detection of MiRNAs with silicon nanowire biosensors. Biosens Bioelectron 24:2504–2508
Chen WY, Chen HC, Yang YS, Huang CJ, Chan HWH, Hu WP (2013) Improved DNA detection by utilizing electrically neutral DNA probe in field-effect transistor measurements as evidenced by surface plasmon resonance imaging. Biosens Bioelectron 41:795–801
Jiang ZY, Jiang XX, Su S, Wei XP, Lee ST, He Y (2012) Silicon-based reproducible and active surface-enhanced Raman scattering substrates for sensitive, specific, and multiplex DNA detection. Appl Phys Lett. doi: 10.1063/1.3701731
Han X, Wang H, Ou X, Zhang X (2012) Highly sensitive, reproducible, and stable SERS sensors based on well-controlled silver nanoparticle-decorated silicon nanowire building blocks. J Mater Chem 22:14127
Bunimovich YL, Shin YS, Yeo WS, Amori M, Kwong G, Heath JR (2006) Quantitative real-time measurements of DNA hybridization with alkylated nonoxidized silicon nanowires in electrolyte solution. J Am Chem Soc 128:16323–16331
Zhang GJ, Chua JH, Chee RE, Agarwal A, Wong SM, Buddharaju KD, Balasubramanian N (2008) Highly sensitive measurements of PNA-DNA hybridization using oxide-etched silicon nanowire biosensors. Biosens Bioelectron 23:1701–1707
Gao A, Lu N, Dai P, Li T, Pei H, Gao X (2011) Silicon nanowire-based CMOS-compatible field-effect transistor nanosensors for ultrasensitive electrical detection of nucleic acids. Nano Lett 11:3974–3978
Gao A, Zou N, Dai P, Lu N, Li T, Wang Y, Zhao J, Mao H (2013) Signal-to-noise ratio enhancement of silicon nanowires biosensor with rolling circle amplification. Nano Lett 13:4123–4130
Su S, Wei X, Zhong Y, Guo Y, Su Y, Huang Q, Lee ST, Fan C, He Y (2012) Silicon nanowire-based molecular beacons for high-sensitivity and sequence-specific DNA multiplexed analysis. ACS Nano 6:2582–2590
Serre P, Ternon C, Stambouli V, Periwal P, Baron T (2013) Fabrication of silicon nanowire networks for biological sensing. Sensors Actuators B Chem 182:390–395
Han SW, Lee S, Hong J, Jang E, Lee T, Koh WG (2013) Mutiscale substrates based on hydrogel-incorporated silicon nanowires for protein patterning and microarray-based immunoassays. Biosens Bioelectron 45:129–135
Zhang GJ, Ning Y (2012) Silicon nanowire biosensor and its applications in disease diagnostics: a review. Anal Chim Acta 749:1–15
Zhang GJ, Huang MJ, Ang JJ, Yao Q, Ning Y (2013) Label-free detection of carbohydrate-protein interactions using nanoscale field-effect transistor biosensors. Anal Chem 85:4392–4397
Zhang ML, Yi CQ, Fan X, Peng KQ, Wong NB, Yang MS, Zhang RQ, Lee ST (2008) A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay. Appl Phys Lett. doi: 10.1063/1.2833695
Miao R, Mu L, Zhang H, Xu H, She G, Wang P, Shi W (2012) Modified silicon nanowires: a fluorescent nitric oxide biosensor with enhanced selectivity and stability. J Mater Chem 22:3348
Zhuo S, Shao M, Xu H, Chen T, Ma DDD, STL (2013) Au-modified silicon nanowires for surface-enhanced fluorescence of Ln3+ (Ln 5 Pr, Nd, Ho, and Er). J Mater Sci 24:324–330
Yan Q, Wang Z, Zhang J, Peng H, Chen X, Hou H, Liu C (2012) Nickel hydroxide modified silicon nanowires electrode for hydrogen peroxide sensor applications. Electrochim Acta 61:148–153
Su S, Wei X, Guo Y, Zhong Y, Su Y, Huang Q, Fan C, He Y (2013) A silicon nanowire-based electrochemical sensor with high sensitivity and electrocatalytic activity. Part Part Syst Charact 30:326–331
Kwon DH, An HH, Kim H-S, Lee JH, Suh SH, Kim YH, Yoon CS (2011) Electrochemical albumin sensing based on silicon nanowires modified by gold nanoparticles. Appl Surf Sci 257:4650–4654
Lee MH, Lee K, Jung SW (2012) Multiplexed detection of protein markers with silicon nanowire FET and sol-gel matrix. Conf Proc IEEE Eng Med Biol Soc 2012:570–573
Wu JY, Tseng CL, Wang YK, Yu Y, Ou KL, Wu CC (2013) Detecting interleukin-1β genes using a N2O plasma modified silicon nanowire biosensor. J Exp Clin Med 5:12–16
Shen F, Wang J, Xu Z et al (2012) Rapid flu diagnosis using silicon nanowire sensor. Nano Lett 12:3722–3730
Aiyelabegan HT, Zaidi SSZ, Fanuel S, Eatemadi A, Ebadi MTK, Sadroddiny E (2016) Albumin-based biomaterial for lungs tissue engineering applications. Int J Polym Mater Polym Biomater 65(16):853-861
Beiranvand S, Eatemadi A, Karimi A (2016) New updates pertaining to drug delivery of local anesthetics in particular bupivacaine using lipid nanoparticles. Nanoscale Res Lett 11:1–10
Daraee H, Eatemadi A, Abbasi E, Fekri Aval S, Kouhi M, Akbarzadeh A (2016) Application of gold nanoparticles in biomedical and drug delivery. Artif Cells Nanomed Biotechnol 44:410–422
Daraee H, Etemadi A, Kouhi M, Alimirzalu S, Akbarzadeh A (2016) Application of liposomes in medicine and drug delivery. Artif Cells Nanomed Biotechnol 44:381–391
Eatemadi A, Darabi M, Afraidooni L, Zarghami N, Daraee H, Eskandari L, Mellatyar H, Akbarzadeh A (2016) Comparison, synthesis and evaluation of anticancer drug-loaded polymeric nanoparticles on breast cancer cell lines. Artif Cells Nanomed Biotechnol 44:1008–1017
Ghafarzadeh M, Eatemadi A, Fakhravar Z (2016) Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro. Cell Mol Biol 2016:102–106
Eatemadi A, Daraee H, Karimkhanloo H, Kouhi M, Zarghami N, Akbarzadeh A, Abasi M, Hanifehpour Y, Joo SW (2014) Carbon nanotubes: properties, synthesis, purification, and medical applications. Nanoscale Res Lett 9:1–13
Eatemadi A, Daraee H, Zarghami N, Melat Yar H, Akbarzadeh A (2016) Nanofiber: synthesis and biomedical applications. Artif Cells Nanomed Biotechnol 44:111–121
Mellatyar H, Akbarzadeh A, Rahmati M, Ghalhar MG, Etemadi A, Nejati-Koshki K, Zarghami N, Barkhordari A (2014) Comparison of inhibitory effect of 17-DMAG nanoparticles and free 17-DMAG in HSP90 gene expression in lung cancer. Asian Pac J Cancer Prev 15:8693–8698
Mohammadian F, Eatemadi A (2016) Drug loading and delivery using nanofibers scaffolds. Artif Cells Nanomed Biotechnol 17:1–8
Seidi K, Eatemadi A, Mansoori B, Jahanban-Esfahlan R, Farajzadeh D (2014) Nanomagnet-based detoxifying machine: an alternative/complementary approach in HIV therapy. J AIDS Clin Res 11:1-10
The authors thank Department of Medical Biotechnology, School of advance Science in Medicine, Tehran University of Medical Sciences and Mechanical engineering, Sharif University of Technology, Tehran, Iran.
PN and HD conceived of the study and participated in its design and coordination. AE supervised the whole study. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.