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Fig. 1 | Nanoscale Research Letters

Fig. 1

From: Potential Suppressive Effects of Two C60 Fullerene Derivatives on Acquired Immunity

Fig. 1

Effects of C60 derivatives on T cell responses. a C60-P or C60(OH)36 was added to responder splenocytes 30 min before the addition of stimulator splenocytes. After incubation of the cells for 4 days, the amount of IL-2 in the supernatants was measured by ELISA. b One week after the third immunization with OVA and alum, mice were euthanized and single-cell suspensions of splenocytes were prepared. C60-P or C60(OH)36 was added to the wells 30 min before the addition of OVA (100 μg/mL). After incubation of the cells for 3 days, the amount of IL-4 in the supernatants was measured by ELISA. c Purified CD4+ T cells were added to anti-CD3-coated plates. Each C60 derivative or N-acetylcysteine (NAC) was added to the wells 30 min before the addition of anti-CD28. After incubation of the cells for 3 days, the amount of IL-2 in the supernatants was determined by ELISA. Data are means ± SDs for three to six independent cultures (n = 3 to 6). *P < 0.05 vs. control group

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