- Nano Express
- Open Access
Colloidal Nanomolybdenum Influence upon the Antioxidative Reaction of Chickpea Plants (Cicer arietinum L.)
© The Author(s). 2016
- Received: 8 September 2016
- Accepted: 13 October 2016
- Published: 26 October 2016
The use of colloidal solutions of metals as micronutrients enhances plant resistance to unfavorable environmental conditions and ensures high yields of food crops. The purpose of the study was a comparative evaluation of presowing treatment with nanomolybdenum and microbiological preparation impact upon the development of adaptive responses in chickpea plants. Oxidative processes did not develop in all variants of the experiment but in variants treated with microbial preparation, and joint action of microbial and nanopreparations even declined, as evidenced by the reduction of thiobarbituric acid reactive substances in photosynthetic tissues by 15 %. The activity of superoxide dismutase increased (by 15 %) in variant “nanomolybdenum” and joint action “microbial + nanomolybdenum,” but it decreased by 20 % in variants with microbial preparation treatment. The same dependence was observed in changes of catalase activity. Antioxidant status factor, which takes into account the ratio of antioxidant to pro-oxidant, was the highest in variants with joint action of microbial preparation and nanomolybdenum (0.7), the lowest in variants with microbial treatment only (0.1). Thus, the results show that the action of nanoparticles of molybdenum activated antioxidant enzymes and decreased oxidative processes, thus promoting adaptation of plants.
- Superoxide dismutase
Plants contain molybdenum in small quantities (0.001–0.1 mg% in terms of dry matter), but it has an important role in phosphorus and protein metabolism. Molybdenum is present in all organs, part of the 20 enzymes (aldehyde oxidases, hydrogenases, nitrate reductase) that catalyze the transition of nitrates into nitrites. It should be noted particularly for its role in the metabolism of legumes because molybdenum is involved in fixing of molecular nitrogen by nodule bacteria of the genus Rhizobium . Formation of legume-rhizobial symbiosis includes a number of stages, where the enzyme complex, nitrogenase, is synthesized. It catalyzes the reduction of molecular nitrogen from the atmosphere . This complex consists of two enzymes: the actual nitrogenase (so-called MoFe protein or dinitrogenase) and dehydrogenase (Fe protein) . The MoFe protein cofactor consists two atoms of molybdenum, which determine the influence of nanomolybdenum colloidal solution on nodulation—central link of legume—and rhizobial symbiosis, providing the necessary conditions for the formation and functioning of the enzyme complex and nitrogen-fixing system [2, 4]. Now, the great interest to the group of biologically active substances—nanosolution of metals (colloidal solutions of metals), owing to the structurally phased condition, gets a set of properties useful to biological objects. The nanoparticles of metals can be the activators of antioxidant protective mechanisms of plants while cultivating in various stressful conditions. There is no single opinion on the impact of metal nanoparticles upon physiological and biochemical processes in plants in available literature—as positive and negative effects are noted. Our previous studies have shown change in element metal content in roots and shoots of winter wheat plants at colloidal solution nanosized particles of metal (Cu, Zn, Fe, Mn) action [5, 6]. Also, we got a positive nanomolybdenum impact upon the root microflora Cicer arietinum L. . Nowadays, electrospark technologies for obtaining nanostructured metals and alloys are the most effective and can meet the requirements of scientific and applied problems .
A colloidal form of metal nanosized particles that have been derived from underwater electric discharge between the conductive granules was used in our studies. Dispersed phase is formed depending upon the discharge circuit in two size ranges—micro- and nanometer ranges. Micro fraction is the result of melting the surface of metal pellets, followed by crystallization; due to its large size, it cannot be used as an effective form of trace elements for use in biological objects. With nanofraction arising from melting and evaporation followed by vapor condensation of average size in the range of 10–150 nm, corresponding structural and phase composition of the solid phase has signs of biological function and can be used in biotechnology, particularly in the technologies of growing vegetable products. With the increase of nanoparticle size or aggregate formation, as well as with increasing degree of oxidation of the metal phase (formation of CuO, Fe3O4, and MnO2 oxides) and amount of oxide phase on particle surface, their biological activity slowed down . A metastable non-equilibrated nanoparticle state cannot allow to predict the physiological and biochemical processes in plants; therefore, these studies remain actual today.
Taking into account the widespread use of microbiological origin preparation to improve the nitrogen fixation of plants, the purpose of our study was a comparative assessment of nanomolybdenum and microbiological preparations of Ryzobofitom impact upon the development of adaptive responses in chickpea plants. Chickpea plants are drought-tolerant and are able to fix atmospheric nitrogen by forming the symbiotic relationships with nitrogen fixation microorganisms that not only meet the requirements of plants in nitrogen but also bring it into the ground .
where m is the concentration of nanoparticles of metal (mg/l) and V is the volume of one mole of metal atoms (cm3/mol). The colloidal solution of nanoparticles of molybdenum was used in the dose of 1 μl per gram (μl/g).
Treatment was performed according to following scheme: (1) control and treatment of water, (2) presowing treatment with molybdenum nanoparticles, (3) presowing treatment with microbiological preparation of Ryzobofitom, and (4) presowing treatment with molybdenum nanoparticles and microbiological preparation of Ryzobofitom.
Extraction of antioxidant enzymes was performed according to Rios-Gonzalez et al. . The activity of superoxide dismutase (SOD; EC 18.104.22.168) was determined by the ability to inhibit recovery of nitroblue tetrazolium (NBT) at λ = 560 nm. The reaction mixture containing 1 ml of riboflavin, 1 ml of methionine, 1 ml NBT, and 50 ml of extract. The unit of enzyme activity was 50 % inhibition of formazan formation. SOD activity is expressed in arbitrary units per mg protein extract .
The activity of catalase (CAT; EC 22.214.171.124) was determined according to Aeby . The reaction mixture contained 2.9 ml of phosphate buffer (pH 7.0), 90 ml of extract, and 10 ml of 33 % H2O2. Measurements were carried out at λ = 240 nm for 60 s. Activity was expressed as arbitrary (arb.) units per mg of protein.
Determining the Level of Lipid Peroxidation
where F is the factor antioxidant status, CATa is the CAT activity, SODa is the SOD activity, and TBARScon is the TBARS content  characterizing antioxidant potential.
Chlorophylls a, b, and carotenoid content were determined by measuring alcohol extract optical density . Determination of protein was performed according to Bradford . The reaction mixture contained 2 ml of Bradford reagent, 16 ml of 0.15 M NaCl and 40 ml of extract. The tubes were left for 2 min; optical density was measured on a spectrophotometer UV-1800 (Shimadzu) at λ = 595 nm. Protein concentration of the extract was calculated according to the calibration curve for bovine albumin.
Statistical analysis of the data was made using program Statistica 6.0. Probability of the difference between the arithmetic mean of indicators was established using Student’s test. The differences are considered to be significant at a value P ≤ 0.05 .
Some investigators found a stimulating effect of metal nanoparticles upon chlorophyll content . Others pay attention to chromosomal aberrations of young plants treated with magnetic nanoparticles, and the level of chlorophyll was increased at their low concentrations and decreased at higher concentrations .
In general, there is an increase in dynamics of chlorophyll a and chlorophyll b while maintaining stable levels of carotenoids or some reduction of their contents. We can assume that treatment with molybdenum nanoparticles contributed to the preservation of pool of chlorophylls by maintaining a certain level of carotenoids, which are able to quench an excited state of chlorophyll and prevent the formation of singlet oxygen and other reactive oxygen species (ROS) . Joint action of nanomolybdenum and microbiological preparation keep stable these indexes appropriate to control variants, but the microbiological preparation treatment only (variant 3) declined them significantly.
Thus, modification of microbial preparation with nanomolybdenum raised its bioactivity, probably due to the fact that metal nanoparticles have excess energy; they are highly reactive and can engage in the process of aggregation. In addition, they can bind to different organelles in cytoplasm thus changing intracellular and physiological features.
Thus, the results point to the indirect effect of molybdenum nanoparticles upon the metabolic processes that lead to activation of antioxidant enzymes and reduction of oxidative processes, thus promoting adaptation of plants.
We thank Prof. Lopatko K.H. (National University of Life and Environmental Sciences of Ukraine) for the colloidal solution of molybdenum nanoparticles given for the experiment.
Publications are based on the research provided by the grant support of the State Fund for Fundamental Research (project N 0116U004779).
NT is the author of the idea, guided the research, and is the corresponding author. LB and OK performed the biochemical assays and calculation of the results and data statistical analysis. OS and MK designed and wrote the first draft of the manuscript. LG performed the field experiments. AO performed the literature data analysis and discussion of the results. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Kaiser BN, Gridley KL, Brady JN, Phillips T, Tyerman SD (2005) The role of molybdenum in agricultural plant production. Ann Bot 96:745–54View ArticleGoogle Scholar
- Volkogon V (2006) Microbial preparations in crop production. Theory and practice. Agrarna nauka, Kyiv, ukrGoogle Scholar
- Schwarz G, Mendel RR, Ribbe MW (2009) Molybdenum cofactors, enzymes and pathways. Nature 460:839–47View ArticleGoogle Scholar
- Priestera JH, Gea Y, Mielkea RE, Horst AM, Moritz SC, Espinosa K et al (2012) Soybean susceptibility to manufactured nanomaterials with evidence for food quality and soil fertility interruption. Proc Natl Acad Sci U S A 109:2451–6View ArticleGoogle Scholar
- Taran N, Batsmanova L, Konotop Y, Okanenko A (2014) Redistribution of elements of metals in plant tissues under treatment by non-ionic colloidal solution of biogenic metal nanoparticles. Nanoscale Res Lett 9:354–7View ArticleGoogle Scholar
- Panyuta O, Belava V, Fomaidi S, Kalinichenko O, Volkogon M, Taran N (2016) The effect of pre-sowing seed treatment with metal nanoparticles on the formation of the defensive reaction of wheat seedlings infected with the eyespot causal agent. Nanoscale Res Lett 11:92View ArticleGoogle Scholar
- Taran N, Gonchar O, Lopatko K, Batsmanova L, Patyka M, Volkogon M (2014) The effect of colloidal solution of molybdenum nanoparticles on the microbial composition in rhizosphere of Cicer arietinum L. Nanoscale Res Lett 9:289–95View ArticleGoogle Scholar
- Lopatko K (2012) Physical modeling the process of formation metal nanoparticles in spark processing. Scientific Bulletin NUBiP Ukraine 174:132–9Google Scholar
- Lopatko KG, Melnychuk MD (2013) Physics, synthesis and biological functionality of nanoscale objects. Publishing center NUBiP Ukraine, Kyiv (ukr)Google Scholar
- Davies S, Turner N, Palta JA, Siddique K, Plummer J (2000) Remobilisation of carbon and nitrogen supports seed filling in chickpea subjected to water deficit. Aust J Agric Res 51:855–66View ArticleGoogle Scholar
- Lopatko KG, Melnichuk MD, Aftandilyants YG, Gonchar EN, Boretskij VF, Veklich AN et al (2013) Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application. Ann Warsaw Univ Life Sci 61:105–15, SGGW AgricultureGoogle Scholar
- Lopatko KG, Aftandilyants EH, Kalenska SM, Tonkha OL. Mother colloidal solution of metals. B01J 13/00 Patent of Ukraine No. 38459 from 12 Jan 2009. http://uapatents.com/4-38459-matochnijj-kolodnijj-rozchin-metaliv.html. Accessed 12 Jan 2009
- Rios-Gonzalez K, Erdei L, Lips SH (2002) The activity of antioxidant enzymes in maize and sunflower seedlings as affected by salinity and different nitrogen sources. Plant Sci 162:923–30View ArticleGoogle Scholar
- Giannopolitis CN, Ries SK (1977) Superoxide dismutase I. Occurrence in higher plants. Plant Physiol 59:309–14View ArticleGoogle Scholar
- Aeby H (1984) Catalase in vitro. Methods Enzymol 105:121–6View ArticleGoogle Scholar
- Kumar GNM, Knowles NR (1993) Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seed-tubers. Plant Physiol 102:115–24View ArticleGoogle Scholar
- Chevari S, Andyal T, Strenger J (1991) Determination of antioxidant blood parameters and their diagnostic value in the elderly. Laboratory work 10:9–13, rusGoogle Scholar
- Lichtethaler HK (1987) Chlorophylls and: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–82View ArticleGoogle Scholar
- Bradford M (1976) A rapid and sensitive methods for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–54View ArticleGoogle Scholar
- Millar N (2001) Biology statistics made using Excel. Sch Sci Rev 83:23–34Google Scholar
- Voica C, Polescu L, Lazar DA (2003) The influence of the magnetic fluids on some physiological processes in Phaseolus vulgaris. Rev Roum Biol 48:9–15Google Scholar
- Racuciu M, Miclaus S, Creanga D (2009) The response of plant tissues to magnetic fluid and electromagnetic exposure. Rom J Biophys 19:73–82Google Scholar
- Martin JE, Herzing AA, Yan W, Li XQ, Koel BE, Kiely CJ et al (2008) Determination of the oxide layer thickness in core-shell zero-valent iron nanoparticles. Langmuir 24:4329–34View ArticleGoogle Scholar
- Sun YP, Li XQ, Cao J, Zhang WX, Wang HP, Sun YP et al (2006) Characterization of zero-valent iron nanoparticles. Adv Colloid Interface Sci 120:47–56View ArticleGoogle Scholar