- Nano Express
- Open Access
Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity
Nanoscale Research Lettersvolume 11, Article number: 520 (2016)
Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm−1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm−1 (C–C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5–50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs.
Shape and size-controlled synthesis of silver nanoparticles using Aloe vera plant extract
Currently, nanoparticle-mediated studies have been predominantly demonstrated and penetrated into the interdisciplinary sciences with nanotechnology due to their potential applications [1–9]. The optical, electronic, catalytic, and electrochemical properties of the nanoparticles are undoubtedly dependent on their size, shape, and reaction medium. It can be synthesized by different methods including wet chemical method, polyol method, microwave synthesis, photochemical approach, and biogenic synthesis, the popular methods in recent days . Biogenic synthesis of nanoparticles is also known to be the green chemistry method using plant origin, edible items, algae, and herbal products [11–14]. The synthesis of silver nanoparticles (AgNP) with different sizes and shapes has been considered in the past with special interest in various fields of research due to their potential to develop for novel biosensors, chemical sensors, electro-optical devices, data storage media, surface-enhanced Raman spectral spectroscopy, and biological imaging [15–19]. AgNP have pronounced to have antimicrobial properties and performed well than other metal nanoparticles [20–25].
Recently, many researchers have been using plant or herbal extracts like neem , Aloe vera , Phyllanthus , turmeric , seaweeds , coffee, and tea  as reducing agents for the synthesis of AgNP. These extracts are not only acting as a reducing agent in the synthesis of nanoparticles but also as a stabilizing agent. In the present investigation, we exploited the usage of Aloe vera plant extract as a reducing agent to synthesize AgNP because of its inherent antimicrobial activity against various microorganisms and gelation properties [32–35]. It is well understood that Aloe vera plant extract can be used to cure various diseases with their biologically important molecules. Researchers have been shown to isolate the bioactive molecules from the Aloe vera plant extract using high-performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC-MS) .
Microwave heating is a fast emerging and widely followed new processing technology for a variety of nanostructured materials with smaller size, narrower size distribution, and spherical, octahedron, rod, and tetrahedron shape [37–42]. In the present study, we utilized the Aloe vera plant extract for the synthesis of AgNP under different conditions. The basic needs of this study are to control the size and shape of AgNP as information on this aspect is very limited. Here, we have shown how the plant extract direct the size and shapes during the growth of AgNP with Aloe vera plant extract under microwave heating and study its catalytic efficiency in antimicrobial activity.
Aloe vera was collected from Chennai city (Tamilnadu, India). Silver nitrate (AgNO3), ammonia solution (25 %), and sodium hydroxide (NaOH) were purchased from SRL chemical, India. The filter discs and nutrient agar were purchased from Merck, Mumbai, India. All the preparations were done by using deionized water. For microbial studies, the sterilized water was used.
Preparation of Aloe vera Plant Extract
A fully grown Aloe vera leaf was taken after washing thoroughly with water and removing the green skin and cutting them into small pieces, and finally, Aloe vera gel was ground to form a colloidal solution. The above colloidal solution was filtered and this solution was used for further analysis.
Synthesis of AgNP
About 5 ml of 10 mM AgNO3 solution was taken into a 50-ml flask containing about 5 ml Aloe vera plant extract and finally diluted with 1 % ammonia solution to adjust the pH of the medium. The reaction mixture was allowed to stand at room temperature and the appearance of orange yellow color indicated the formation of spherical shaped AgNP.
Synthesis of Ag Octahedron Under Microwave Irradiation
About 20 mg of AgNO3 was discharged into a 10 ml beaker containing different concentrations of Aloe vera plant extract, and 0.5 ml of 25 % ammonia solution was added to adjust the pH of the medium. Here, we have chosen three different concentrations of silver nitrate for the synthesis of Ag–Oh nanoparticles. These solutions were kept in microwave oven for a period of 60 s with an appropriate power supply, and the reaction mixture was allowed to cool until reaching the room temperature. Again, the reaction mixture was heated further for another 60 s. These heating and cooling steps were repeated three or four times to obtain a uniform-sized octahedral-shaped AgNP. Because of the intermittent heating and cooling processes, the initially formed spherical-shaped nanoparticles act as seeds for further growth of the octahedral-shaped AgNP. In this nucleation process Aloe vera gel plays an important role to regulate size and shape of the AgNP.
The synthesized AgNP was tested for antibacterial activity using agar disc diffusion assay method as per Clinical Laboratory Standards Institute (CLSI). Briefly, 0.5 McFarland of inoculums were made using the overnight grown bacterial strains. Strains of microorganism used in this study were gram positive and gram negative bacteria. They were diluted as 1:10 to get final inoculums of 1 × 107 colony forming unit per milliliter (CFU/ml) and inoculated on the Mueller-Hinton agar (BD Difco) medium. Sterile discs of 6 mm width were dipped into the extract, AgNP solution, and extract stabilized AgNP as well as the assay control antibiotics. Discs were placed over the incubated plates followed by incubation overnight at 37 °C. The antibacterial activity was assigned by measuring the zone of inhibition (mm) formed around the disc.
Optical, Structural, and Imaging Studies
The biogenic synthesis of AgNP was monitored by a UV-visible spectrophotometer (Shimadzu, UV-1800, Japan). The progress of the reaction and the appearance of plasmon band were monitored at different time intervals. AgNP was dried and ground (with KBR pellets) and analyzed by Fourier transform infrared spectroscopy (FTIR; Perkin Elmer Spectrum 65). All spectra were nullified against the background spectrum obtained with KBr. The X-ray powder diffraction (XRD) pattern (Bruker D8 Advance) was recorded by using Cu-Kα1 radiation (λ of 1.5406 Å) and nickel monochromator filtering wave tube voltage (40 kV) and tube current (30 mA). The scanning was done in the region of 2θ (from 0o to 80o) at 0.02o/min. The size and shape of the AgNP was analyzed by transmission electron microscopy (TEM), and the images were obtained by JEM-1200EX instrument operated at accelerating voltage (120 kV). The particle size was analyzed by scanning electron microscopy (SEM) (FEI Quanta FEG 200). Size and shape-controlled nanoparticles were studied in a microwave oven (National model 64 N.N-GD 576 M). The sample was subjected to several short bursts of microwave irradiation at the desired frequency (2.45 GHz), at a power output of ~100 W in a cyclic mode (on 5 s, off 5 s) to avoid overheating and metal aggregation. The irradiation process was conducted as a minimum of five and maximum of 15 cycles. Surface-enhanced Raman spectroscopy/Raman scattering (SERS) was performed by Real-Time Analyzers, Inc. that incorporates a high-performance 785 nm spectrometer with RTA’s patented SERS vials for the detection of trace chemicals.
Results and Discussion
The synthesis of nanoparticles benefits for the development of clean, non-toxic and environmentally acceptable “green chemistry procedures” and involves a wide range of materials including plant sources [10–14]. Among different nanoparticles that have been proposed, AgNP was found to be with significant applications [24, 32, 34]. Different groups have been involved in synthesizing various sizes and shapes of nanoparticles under microwave condition using high boiling solvents . Here, we report size and shape-controlled synthesis of AgNP using Aloe vera plant extract wherein the Aloe vera gel plays a vital role as a shape-directing agent for the growth of AgNP under microwave irradiation. The chemistry involved in the formation of AgNP is displayed in Fig. 1. For the initial assessments, we have generated AgNP under room temperature and evaluated by UV-Visible spectroscopy.
UV-Visible Spectroscopic Analysis
Figure 2 shows the UV-Visible spectra of AgNP grown with different time intervals at room temperature in alkaline pH medium. The progress of the reaction is facile when ammoniacal silver nitrate was used as a starting material in the presence of freshly collected Aloe vera gel, and here it was proved that the reaction is faster in alkaline pH medium. A sharp peak increment in absorbance value was noted during the addition of silver nitrate solution, and then after reaching the maximum absorbance, the reaction slowed down due to the complete reduction of Ag+ to Ag (0). The plasmon peak appeared at 420 nm, which indicates the formation of AgNP. After a few hours, the attainment of peak becomes stabilized (Fig. 2; inset). No obvious change was noticed in 3 h due to saturation in 2 h. However, as the duration of reaction increases, more amounts of AgNPs were formed. In order to optimize the experiment, the kinetics of the particle growth was investigated. Based on the preliminary preparation and assessment at room temperature, further synthesis was preceded by microwave heating.
Microwave Synthesis of AgNP
Polyol method is routinely followed to synthesize various sizes and shapes of AgNP using polyvinylpyrrolidone (PVP) as a shape-directing agent. Here, we reported that the Aloe vera plant extract can be used as a size and shape-directing agent for the preparation of octahedron-shaped AgNP. The addition of silver nitrate solution into Aloe vera plant extract yielded a spherical shaped AgNP, whereas the reaction mixture subjected to heat under microwave results in the formation of octahedron-shaped AgNP. In order to avoid the fast evaporation of solvent, the reaction mixture was kept in a microwave oven for a period of 1 min and allowed to cool, and the heating and cooling cycles were continued three to four times. The color change during the microwave heating at different intervals was monitored by UV-Visible spectral studies. The UV-Visible absorption spectrum of the octahedral-shaped AgNP is shown in Fig. 3. From the figure, three major shoulder bands were observed at 300, 350, and 430 nm ranges. The appearance of three major shoulder peaks clearly indicates the multiple electromagnetic interactions with the octahedral-shaped AgNP. This preparation was also carried out with different amounts of AgNO3 and shown in Fig. 3; the profile of the spectra was increased with the increment of AgNO3, which indicates the concentration-dependent synthesis of AgNP. At room temperature, the SPR value was higher than that obtained in the case of microwave-assisted synthesis. It is observed that the SPR band shifts to a longer wavelength with increasing particle size. The main attraction of microwave synthesis is that it yields small, uniform-sized nanoparticles in much lesser reaction time. The speedy consumption of starting materials reduces the formation of agglomerates in microwave-assisted methods and provides nanoparticles with narrow size distribution.
The obtained particles were observed under field-emission scanning electron microscopy (FE-SEM). Based on this observation, the surface morphology and the shape of AgNP were further confirmed from the resulting images with the octahedral-shaped AgNP (Fig. 4). These particles have narrow sizes and are distributed with the average size of 5–50 nm. Further, for confirmation and to support the FE-SEM results, we also observed the images of AgNP by TEM, and a clear image of AgNP was visualized (Fig. 5).
For structural analyses, XRD and FTIR studies were carried out. The XRD pattern for the Aloe vera plant extract in the presence of AgNP is shown in Fig. 6. The XRD peaks were indexed and represented the fcc pattern of AgNP. The displayed peak indicates the nanocrystalline shaped silver particles. The FTIR spectrum of AgNP protected with Aloe vera plant extract is shown in Fig. 7. The observed peaks indicate the occurrence of flavanones and terpenoids that are plenty in Aloe vera plant extract . The peaks were observed with AgNP at 1587.6 cm−1 (C=C groups or from aromatic rings), 1386.4 cm−1 (geminal methyls), and 1076 cm−1 (ether linkages) suggest the presence of flavanones or terpenoids on the surface of synthesized AgNP.
Surface-Enhanced Raman Scattering (SERS) Study
When molecules are on nanostructured silver or gold surfaces, they can experience the enhancement of Raman scattering, the phenomenon known as SERS. SERS-active substrates include electrochemically roughened metals, suspensions of metal nanoparticles or nanoparticles adsorbed on the surfaces, lithographically produced metal nanostructures, and vacuum-deposited metal island films. On the other hand, nanoparticles with different shapes and sizes have also shown an enhancement factor that is one of the important factors for the sensing and imaging applications [43–46]. The SERS data for the 4-aminothiophenol modified octahedral shaped AgNP are shown in Fig. 8. The appearance of the strong intense peak is due to the interaction between the electromagnetic radiations of AgNP with the probe molecules. The SERS spectrum of AgNPs displaying a group of vibrational bands were at 1594, 1082, and 474 cm−1. The main position band was at 1594 cm−1, corresponding to the C–C stretching vibration and the peaks were at 1082 cm-1, attributed to the C–H stretching vibration, whereas weak peak was appearing at 474 cm−1, corresponding to the N–H bending vibration, CNC symmetric stretching, and CO bending vibration.
To study the SERS ability of octahedral-shaped AgNP modified substrate, the SERS enhancement factor (EF) for 4-aminothiophenol (4-ATP) was estimated according to the following formula:
where ISERS and Ibulk are the vibration intensities in the SERS and normal Raman spectra of 4-ATP, respectively. Nsurf and Nbulk are the number of molecules for SERS, and the number of molecules for the bulk sample under laser illumination, respectively. The Nsurf and Nbulk values can be calculated on the basis of the estimated concentration of the surface species or bulk sample and the sample areas. In our experiment, 5 μl of 1 × 10−9 M 4-ATP solution was pipetted on the Ag–Oh NPs modified substrate, and after the droplet evaporated in air, the Raman spectrum was recorded. The resulting enhanced Raman spectrum is shown in Fig. 8. The spectral pattern is assigned for the self-assembly of 4-ATP on the surface of the octahedral AgNP. Other than major peaks, we can also see some less intense peaks which are due to Raman spectra data of the biopolymers and some of the small molecules present along the Ag–Oh NPs.
The main idea to study the EF is how the molecular signal is enhancing and how many folds. 4-aminothiophenol, 4-mercaptopyridine, and crystal violet were used as probes to study the enhancement factor. We used these compounds in SERS study to control the particle shape and size and to accurately tune nanoparticles for a specific localized surface plasmon resonance peak for optimized EF. Shape also influences SERS enhancement due to locations of high curvature such as sharp corners or tips. These locations produce unusually large electromagnetic enhancement referred as lightning rod effect; the electric field induced at the tip will be much stronger than other areas on the surface. The influence of the lightning rod effect can be observed near high curvature points on different shapes.
Since Aloe vera plant extract has been widely used to treat various diseases, we are interested to test its antimicrobial activity against different microorganisms. The antibacterial activity of Aloe vera plant extract with AgNP was compared to that of AgNP-free extract and EDTA-capped AgNP (synthesized by EDTA as reducing agent). The Ag concentration was adjusted to be identical for each sample. Among the tested samples, AgNPs@Aloe vera was shown to have the largest zone of inhibition around the discs, indicating the occurrence of significant antibacterial activity. Though, the mechanism of antibacterial activity of AgNP was not yet clearly understood, but the generalized mechanism is that the interaction of silver ion with the phosphorus moieties present in DNA of microorganism, resulting in the inactivation of DNA replication. The summary of antimicrobial activity of AgNP synthesized using Aloe vera plant extract and EDTA-capped AgNP synthesized by chemical reduction and Aloe vera plant extract are shown in Table 1. The inhibition studies were performed against different bacterial species including Staphylococcus aureus, Bacillus cereus, Micrococcus luteus, Escherichia coli, Klebsiella pneumoniae. These analyses against pathogenic bacteria have clearly displayed different inhibition rates in each case. Comparison between inhibitions by AgNP synthesized using Aloe vera plant extract and controls (only AgNP and only Aloe vera plant extract) revealed that there are two to four folds higher inhibition rate with the synthesized AgNP using Aloe vera plant extract. Comparisons among inhibitions are displayed in Additional file 1: Figure S1. The values were determined based on statistical standard deviation with the triplicate values. From these data, the AgNP using aloe vera extract (biological synthesis) show a significant activity compared to all the other samples.
The UV-visible spectrum of the interacting behavior of the active component present in the Aloe vera with the freshly prepared AgNP was analyzed (Fig. 9). Figure 9 shows the UV-visible spectra of Aloin, Aloin-stabilized AgNP (biogenic method), and pure AgNP synthesized by chemical reduction method. For Aloin, the absorbance peaks appear at 268, 295, and 352 nm. The sharp peak appearing at 406 nm indicates the formation of AgNP stabilized by Aloin, and the broad peak appearing at 438 nm indicates the formation of pure AgNP synthesized by chemical reduction method. Then, the antibacterial activities against Aloin, AgNP-stabilized Aloin, and pure AgNP (chemical method) were compared and the zone of inhibition was calculated. The calculated standard deviations were between ±1 and ±2. Among these, AgNP stabilized aloin exhibits highest zone of inhibition values which indicates an enhanced antibacterial activity. Hence, these kinds of combined forms of herbal medicine can be used to treat the antibiotic-resistant bacterial strains.
The nano-sized AgNPs as antimicrobial agents have become more common as technological advances make their production more economical. One of the potential applications in which silver can be utilized is in management of plant diseases. Since silver displays multiple modes of inhibitory action to microorganisms, it may be used for controlling various plant pathogens in a relatively safer way compared to synthetic fungicides. The oxidation state of nanosilver strongly influences its ion leaching, and therefore, nanosilver exhibited much stronger antibacterial activity. Furthermore, the Ag+ ion releases about 5 and 60-nm silver particles, as well as macro-sized silver foil in the Ag-exposed surface area. So, antibacterial activity of nanosilver against gram positive and gram negative bacteria is better expressed by surface area, rather than mass or concentrations. Nano-scaled silver may exert these potentials: (1) releases silver ions and generates reactive oxygen species (ROS); (2) interacts with membrane proteins and influences their function; (3) accumulates in the cell membrane, affecting membrane permeability; and (4) enters into the cell where it can generate ROS, release silver ions, and affect DNA.
Silver nanoparticles (AgNP) have been attested with the versatile preparation methods towards their potential applications in interdisciplinary sciences. With the current study, an additional strength has been given for the preparation using Aloe vera gel, as it is one of the important sources for the synthesis of spherical as well as octahedron-shaped AgNP. Methods were demonstrated by different synthetic methodologies (room temperature growth and microwave heating). The antimicrobial activities of the synthesized AgNP were tested against different Gram-negative and Gram-positive bacteria. The results claimed that the enhanced and synergetic activity of Aloe vera plant extract was obtained when it combined with AgNP. This study delineates an excellent SERS substrate with octahedron-shaped AgNP that can be synthesized by microwave heating using Aloe vera gel, which is one of the potential size and shape-directing agents.
- AgNO3 :
Field-emission scanning electron microscopy
Fourier transform infrared spectroscopy
Gas chromatography–mass spectrometry
High-performance liquid chromatography
Scanning electron microscopy
Surface-enhanced Raman spectroscopy/Raman Scattering
Transmission electron microscopy
X-ray powder diffraction
Antoine TE, Hadigal SR, Yakoub AM et al (2016) Intravaginal zinc oxide tetrapod nanoparticles as novel immunoprotective agents against genital herpes. J Immunol 196:4566–4575. doi:10.4049/jimmunol.1502373
Hölken I, Hoppe M, Mishra YK et al (2016) Complex shaped ZnO nano-and microstructure based polymer composites: mechanically stable and environmentally friendly coatings for potential antifouling applications. Phys Chem Chem Phys 122:50–58. doi:10.1016/j.compscitech.2015.11.002
Gopinath SCB, Awazu K, Fujimaki M (2012) Waveguide-mode sensors as aptasensors. Sensors 12:2136–2151. doi:10.3390/s120202136
Gopinath SCB, Awazu K, Fujimaki M, Shimizu K (2013) Evaluation of anti-A/Udorn/307/1972 antibody specificity to influenza A/H3N2 viruses using an evanescent-field coupled waveguide-mode sensor. PLoS ONE 8:e81396. doi:10.1371/journal.pone.0081396
Gopinath SCB, Lakshmipriya T, Awazu K (2014) Colorimetric detection of controlled assembly and disassembly of aptamers on unmodified gold nanoparticles. Biosens Bioelectron 51:115–123. doi:10.1016/j.bios.2013.07.037
Prabakaran E, Pandian K (2015) Amperometric detection of Sudan I in red chili powder samples using Ag nanoparticles decorated graphene oxide modified glassy carbon electrode. Food Chem 166:198–205. doi:10.1016/j.foodchem.2014.05.143
Addanki S, Jayachandiran J, Pandian K, Nedumaran D (2015) Development of optical sensors for the quantitative detection of ozone using gold and silver thin film nanoislands. Sensors Actuators B Chem 210:17–27. doi:10.1016/j.snb.2014.12.071
Jin NZ, Anniebell S, Gopinath SCB, Chen Y (2016) Variations in spontaneous assembly and disassembly of molecules on unmodified gold nanoparticle. Nanoscale Res Lett 11:399
Citartan M, Gopinath SCB, Tominaga J, Tang T (2013) Label-free ways of reporting biomolecular interactions by optical biosensor. Analyst 138:3576–3592
Hebbalalu D, Lalley J, Nadagouda MN, Varma RS (2013) Greener techniques for the synthesis of silver nanoparticles using plant extracts, enzymes, bacteria, biodegradable polymers, and microwaves. ACS Sustain Chem Eng 1:703–712. doi:10.1021/sc4000362
Jha AK, Prasad K, Kumar V, Prasad K (2009) Biosynthesis of silver nanoparticles using eclipta leaf. Biotechnol Prog 25:1476–1479. doi:10.1002/btpr.233
Raveendran P, Fu J, Wallen SL (2003) Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 125:13940–13941. doi:10.1021/ja029267j
Raveendran P, Fu J, Wallen SL (2006) A simple and “green” method for the synthesis of Au, Ag, and Au–Ag alloy nanoparticles. Green Chem 8:34. doi:10.1039/b512540e
Xiong J, Wang Y, Xue Q, Wu X (2011) Synthesis of highly stable dispersions of nanosized copper particles using l-ascorbic acid. Green Chem 13:900. doi:10.1039/c0gc00772b
Mishra YK, Mohapatra S, Kabiraj D et al (2007) Synthesis and characterization of Ag nanoparticles in silica matrix by atom beam sputtering. Scr Mater 56:629–632. doi:10.1016/j.scriptamat.2006.12.008
Mishra YK, Mohapatra S, Chakravadhanula VSK et al (2010) Synthesis and characterization of Ag-polymer nanocomposites. J Nanosci Nanotechnol 10:2833–2837. doi:10.1166/jnn.2010.1449
Mohapatra S, Mishra YK, Ghatak J et al (2008) Surface plasmon resonance of Ag nanoparticles embedded in partially oxidized amorphous Si matrix. J Nanosci Nanotechnol 8:4285–4289. doi:10.1166/jnn.2008.AN30
Yang J, Pan J (2012) Hydrothermal synthesis of silver nanoparticles by sodium alginate and their applications in surface-enhanced Raman scattering and catalysis. Acta Mater 60:4753–4758. doi:10.1016/j.actamat.2012.05.037
Bankura K, Maity D, Mollick MMR et al (2014) Antibacterial activity of Ag-Au alloy NPs and chemical sensor property of Au NPs synthesized by dextran. Carbohydr Polym 107:151–157. doi:10.1016/j.carbpol.2014.02.047
Zhao X, Xia Y, Li Q et al (2014) Microwave-assisted synthesis of silver nanoparticles using sodium alginate and their antibacterial activity. Colloids Surfaces A Physicochem Eng Asp 444:180–188. doi:10.1016/j.colsurfa.2013.12.008
Dwivedi S, Saquib Q, Al-Khedhairy AA et al (2015) Rhamnolipids functionalized AgNPs-induced oxidative stress and modulation of toxicity pathway genes in cultured MCF-7 cells. Colloids Surfaces B Biointerfaces 132:290–298. doi:10.1016/j.colsurfb.2015.05.034
Velusamy P, Das J, Pachaiappan R et al (2015) Greener approach for synthesis of antibacterial silver nanoparticles using aqueous solution of neem gum (Azadirachta indica L.). Ind Crops Prod 66:103–109. doi:10.1016/j.indcrop.2014.12.042
Velusamy P, Su C-H, Venkat Kumar G et al (2016) Biopolymers regulate silver nanoparticle under microwave irradiation for effective antibacterial and antibiofilm activities. PLoS ONE 11:e0157612. doi:10.1371/journal.pone.0157612
Bupesh G, Manikandan E (2016) Enhanced antibacterial, anticancer activity from terminalia chebula medicinal plant rapid extract by phytosynthesis of silver nanoparticles core-shell structures. J Nanomed Nanotechnol 07:1–6. doi:10.4172/2157-7439.1000355
Hybridization AN, Zn I (2016) A novel hybridization indicator Zn2(ET)(pzdc)(μ3-OH) for the electrochemical determination of breast cancer gene BRCA1. J Adv Electrochem 2:70–74
Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502. doi:10.1016/j.jcis.2004.03.003
Chandran SP, Chaudhary M, Pasricha R et al (2006) Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog 22:577–583. doi:10.1021/bp0501423
Kasthuri J, Kathiravan K, Rajendiran N (2009) Phyllanthin-assisted biosynthesis of silver and gold nanoparticles: a novel biological approach. J Nanoparticle Res 11:1075–1085. doi:10.1007/s11051-008-9494-9
Shameli K, Bin AM, Zamanian A et al (2012) Green biosynthesis of silver nanoparticles using Curcuma longa tuber powder. Int J Nanomedicine 7:5603–5610. doi:10.2147/IJN.S36786
Kannan RRR, Stirk WA, Van Staden J (2013) Synthesis of silver nanoparticles using the seaweed Codium capitatum P.C. Silva (Chlorophyceae). South African J Bot 86:1–4. doi:10.1016/j.sajb.2013.01.003
Nadagouda MN, Varma RS (2008) Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract. Green Chem 10:859. doi:10.1039/b804703k
Xiu Z, Zhang Q, Puppala HL et al (2012) Negligible particle-specific antibacterial activity of silver nanoparticles. Nano Lett 12:4271–4275. doi:10.1021/nl301934w
Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145:83–96. doi:10.1016/j.cis.2008.09.002
Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83. doi:10.1016/j.biotechadv.2008.09.002
Tsuji M, Hashimoto M, Nishizawa Y et al (2005) Microwave-assisted synthesis of metallic nanostructures in solution. Chem - A Eur J 11:440–452. doi:10.1002/chem.200400417
Saccù D, Bogoni P, Procida G (2001) Aloe exudate: characterization by reversed phase HPLC and headspace GC-MS. J Agric Food Chem 49:4526–4530. doi:10.1021/jf010179c
Hu B, Wang SB, Wang K et al (2008) Microwave-assisted rapid facile “green” synthesis of uniform silver nanoparticles: Self-assembly into multilayered films and their optical properties. J Phys Chem C 112:11169–11174. doi:10.1021/jp801267j
Horikoshi S, Abe H, Torigoe K et al (2010) Access to small size distributions of nanoparticles by microwave-assisted synthesis. Formation of Ag nanoparticles in aqueous carboxymethylcellulose solutions in batch and continuous-flow reactors. Nanoscale 2:1441–1447. doi:10.1039/c0nr00141d
Mittal AK, Chisti Y, Banerjee UC (2013) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31:346–356. doi:10.1016/j.biotechadv.2013.01.003
Zhu Y-J, Chen F (2013) Microwave-assisted preparation of inorganic nanostructures in liquid phase.. doi:10.1021/cr400366s
Nirmala Grace A, Pandian K (2007) One pot synthesis of polymer protected Pt, Pd, Ag and Ru nanoparticles and nanoprisms under reflux and microwave mode of heating in glycerol—a comparative study. Mater Chem Phys 104:191–198. doi:10.1016/j.matchemphys.2007.03.009
El-Rafie HM, El-Rafie MH, Zahran MK (2013) Green synthesis of silver nanoparticles using polysaccharides extracted from marine macro algae. Carbohydr Polym 96:403–410. doi:10.1016/j.carbpol.2013.03.071
Patel DK, Patel K, Dhanabal SP (2012) Phytochemical standardization of Aloe vera extract by HPTLC techniques. J Acute Dis 47–50. doi: 10.1016/S2221-6189(13)60054-2
Orendorff CJ, Gole A, Sau TK, Murphy CJ (2005) Surface-enhanced Raman spectroscopy of self-assembled monolayers: sandwich architecture and nanoparticle shape dependence. Anal Chem 77:3261–3266. doi:10.1021/ac048176x
Citartan M, Gopinath SCB, Tominaga J, Tan SC, Tang TH (2012) Assays for aptamer-based platforms. Biosens Bioelectron 34:1–11
Gopinath SCB, Tang TH, Chen Y, Citartan M, Lakshmipriya T (2014) Bacterial sensing: from microscope to smartphone. Biosens Bioelectron 60:332–342
Authors are grateful to Archit Pharmaceutical, Pvt Ltd, Chennai, India, for providing the infrastructural facilities to carry out the research program. One of the authors (Dr. KP) are grateful to Dr. T.R. Ravindran, Senior Scientist, Condensed Matter Physics Division, Indira Gandhi Centre for Atomic Research, Kalpakkam – 603 102 for SERS analysis.
This study was supported by the High Impact Research grants from University of Malaya MoE GrantUM.C/625/1/HIR/MOHE/MED/16/5, UM.C/625/1/HIR/MOE/DENT/09 and University of Malaya Research Grants (UMRG) RG454-12HTM.
KL and KP carried out the main part of the experiment work and XRD measurements. KL and AJR carried out the FTIR, SEM, and TEM analyses. KL, AJR, SCBG, and KP performed UV and biological analyses. YC and KP performed the supporting experiments. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Ethics Approval and Consent to Participate
This study is not involved with any compliance with ethical issues.
Comparison among inhibitions by AgNP (by chemical method), AgNP with Aloe vera plant extract, only Aloe vera plant extract and only AgNO3 solution. 1. Staphylococcus aureus; 2. Bacillus cereus; 3. Micrococcus luteus; 4. Escherichia coli; 5. Klebsiella pneumoniae. The values were determined based on statistical standard deviation with the triplicate values. The calculated standard deviations were between ±1 and ±2. (DOCX 84 kb)