Fig. 4

Free-body diagram of the centrifuge tube positioning during the centrifugal process and its mathematical derivation. (\( {F}_x= \) horizontal force, \( m= \) mass, \( {a}_x= \) horizontal acceleration, \( {F}_{cp}= \) centripetal force, \( \theta = \) angle, \( \omega = \) rotational speed, \( T= \) tension force, \( r= \) radius and \( {F}_y= \) vertical force, \( g= \) gravity)