- Nano Express
- Open Access
Biosensors Based on Nano-Gold/Zeolite-Modified Ion Selective Field-Effect Transistors for Creatinine Detection
© The Author(s). 2017
- Received: 22 December 2016
- Accepted: 22 February 2017
- Published: 2 March 2017
The combination of advantages of using zeolites and gold nanoparticles were aimed to be used for the first time to improve the characteristic properties of ion selective field-effect transistor (ISFET)-based creatinine biosensors. The biosensors with covalently cross-linked creatinine deiminase using glutaraldehyde (GA) were used as a control group, and the effect of different types of zeolites on biosensor responses was investigated in detail by using silicalite, zeolite beta (BEA), nano-sized zeolite beta (Nano BEA) and zeolite BEA including gold nanoparticle (BEA-Gold). The presence of gold nanoparticles was investigated by ICP, STEM-EDX and XPS analysis. The chosen zeolite types allowed investigating the effect of aluminium in the zeolite framework, particle size and the presence of gold nanoparticles in the zeolitic framework.
After the synthesis of different types of zeolites in powder form, bare biosensor surfaces were modified by drop-coating of zeolites and creatinine deiminase (CD) was adsorbed on this layer. The sensitivities of the obtained biosensors to 1 mM creatinine decreased in the order of BEA-Gold > BEA > Nano BEA > Silicalite > GA. The highest sensitivity belongs to BEA-Gold, having threefold increase compared to GA, which can be attributed to the presence of gold nanoparticle causing favourable microenvironment for CD to avoid denaturation as well as increased surface area. BEA zeolites, having aluminium in their framework, regardless of particle size, gave higher responses than silicalite, which has no aluminium in its structure. These results suggest that ISFET biosensor responses to creatinine can be tailored and enhanced upon carefully controlled alteration of zeolite parameters used to modify electrode surfaces.
- Creatinine deiminase
- Gold nanoparticle
Creatinine is one of the most important analytes used for determination of kidney and muscular dysfunction and control of patients receiving hemodialysis. Its concentration in serum can rise from 35–140 μM to 1 mM during nephron malfunction. However, it can fall below 40 μM due to decreasing of muscle mass [1, 2]. It is usually determined by a spectrophotometric method based on the Jaffe’s reaction despite the high volume of sample and the poor selectivity of this method. HPLC and capillary electrophoresis have been also used for clinical determination of creatinine [3, 4]. These methods require expensive instruments, time-consuming sample pre-treatment and skilled persons to operate them. In addition, sensors and biosensors for creatinine determination have been proposed to reduce cost, time and complexity of routine analysis of biological fluids by being fast, reliable and portable methods allowing home testing. Creatinine biosensors developed are mostly based on either amperometric or potentiometric detection methods [5, 6]. The sensing performance of these biosensors is greatly affected by the immobilization of the bioselective element onto the transducer surface. The conventional methods of enzyme immobilization are physical adsorption, covalent binding, cross-linking and enzyme entrapment with polymers. These methods, however, may suffer from low reproducibility and poor spatially controlled deposition. Recently, inorganic materials such as clays, sol-gels and zeolites have been investigated to solve these problems.
Zeolites are inorganic solids with large surface areas and well-defined internal structures of uniform cages, cavities or channels of monodisperse dimensions . In the field of biosensors, zeolites are promising materials for enzyme immobilization since they have a large surface area, thermal/mechanical stabilities, ion exchange capacity and controllable hydrophilicity/hydrophobicity. Recently, there have been an increasing number of researches on zeolite containing biosensors such as conductometric biosensors based on zeolite immobilized urease , natural clinoptilolite  and amperometric biosensor based on dealuminated zeolite . Additionally, Phadare et al. presented that conjugation of gold nanoparticle and zeolite leads to enhanced biocatalytic activity compared to the free enzyme in solution. They believed that the interaction of gold nanoparticles and oxide zeolite particles have an inductive effect on the bioactivity of protein. This study also resulted in enhanced pH and temperature stability .
Gold nanoparticles have different properties from its bulk size such as biocompatibility, high specific surface area, high surface energy and high conductivity. They also offer numerous adsorption sites to enzymes, antibodies and proteins, which make them an ideal choice for biosensors . Crumbliss et al. used colloidal gold as an immobilization matrix for the development of amperometric biosensor to detect glucose. They concluded that enzymes are tightly adsorbed onto gold nanoparticles and these nanoparticles provide a biocompatible surface that is suitable for immobilizing active enzymes onto electrodes. The same enzymes were shown to denature on bulk metal surfaces [13, 14]. Despite these advantages, there are only a few articles using gold nanoparticles in the field-effect transistor-based biosensors [15–19].
We have previously shown the development of new methods for potentiometric [20, 21], amperometric  and conductometric [8, 23] transducers based on immobilization of enzymes on zeolites. In our recent study, owing to the unique advantages such as easiness, fastness, inexpensiveness and ability to analyse the biological content as electrical signal of electrochemical biosensors with an additional advantage of miniaturized silicon-based semiconductor nature of field-effect transistor (FET)-based sensors, we have developed a novel approach for constructing an ion selective field-effect transistor (ISFET) device for creatinine monitoring using silicalite . The objective of this study was to combine the advantages of using zeolites with different properties (i.e. structure and particle size) with an additional benefit of the ability to use zeolites as hosts for gold nanoparticles to be used for the first time to improve the characteristic properties of ISFET-based creatinine biosensors.
Tetrapropylammonium hydroxide (TPAOH; 25% in water), tetraethyl orthosilicate (TEOS; 98%) from Acros Organics, sodium aluminate (anhydrous, Fisher), sodium hydroxide (97%, J.T. Baker), tetraethylammonium hydroxide (TEAOH; Aldrich, 35% in water), Ludox HS-40 colloidal silica (SiO2, Sigma-Aldrich, 40% in water) and aluminium isopropoxide (98%, Aldrich) were used for zeolite synthesis. For the ion exchange procedure, gold(III)chloride (Aldrich) and sodium borohydride (Sigma-Aldrich) were used.
For biosensor studies, enzyme creatinine deiminase (EC 188.8.131.52) with the activity of 36 U/mg, bovine serum albumin (BSA; fraction V), glycerol 25% aqueous solution of glutaraldehyde (GA) and creatinine were purchased from Sigma-Aldrich. Diethylaminoethyl dextran (DEAE-Dextran) was from Fluka Biochemica and lactitol was from Fluka. The working buffer solution (KH2PO4-NaOH), pH 7.4, was prepared from reagents of Helicon.
Design of Potentiometric Transducer and Measuring System
The synthesized zeolites were characterized by powder X-ray diffraction (XRD) using Rigaku-Ultima IV. Scanning electron microscopy (SEM) analyses were performed in a 400 Quanta FEI. Particle sizes of silicalite and nano BEA were estimated from SEM images whereas for zeolite BEA, Malvern Mastersizer 2000 was used. Cross-sectional SEM images were taken from zeolite-coated silicon wafers. High-angle annular dark-field (HAADF) STEM images of gold-BEA zeolite was obtained using a JEM JEOL 2100F electron microscope equipped with a field emission gun and operated at 200 kV with a STEM detector. This observation was coupled with EDX investigations for elemental analysis using Oxford Energy Dispersive Spectroscopy. The nitrogen adsorption/desorption experiments were carried out at by a Autosorb 6 series (Quantachrome Instruments) instrument. The surface area of the samples was obtained by Multipoint BET, while external surface area was obtained by a t-plot method. Sample preparation method includes outgassing samples under vacuum at 300 °C for 4 h before analysis. The elemental analysis of ion-exchanged and reduced samples was determined using Perkin Elmer Optima 4300DV ICP-OES. X-ray photo-electron spectroscopy (XPS) analysis was carried out on a PHI 5000 VersaProbe spectrometer with an Al-Kα radiation source. The binding energies were referenced to the internal standard C 1s binding energy at 284.5 eV. Contact angles, Ɵ, were measured from electrode surfaces using static sessile water drop method with an Attension Theta goniometer. For each sample, at least five measurements were made and the measurements were taken immediately after the drop had been deposited on the surface. The average angle was calculated by using the OneAttension software from both the left and right sides of the droplet. The standard error in Ɵ was approximately ±2°. Atomic force microscopy was performed in air on a Veeco MultiMode V AFM operated in tapping mode. A silicon tip was used with a scan rate of 1–2 Hz. For AFM measurements, zeolite-coated silicon wafers were used.
Preparation of Biosensors for Creatinine Detection
Synthesis of Silicalite
The optimized molar composition of the gel used for synthesis of silicalite-1 is 1 TPAOH : 4 TEOS : 350 H2O. The alkali source used was TPAOH and the silica source was TEOS. TEOS was added to TPAOH solution under vigorous stirring. The mixtures were aged at room temperature for 6 h under stirring. The gel was introduced into Teflon-lined autoclaves for crystallization. Static synthesis was carried out for 1 day at 125 °C. Product was centrifuged three times at 7500 rpm and dried overnight at 50 °C. The particle size of silicalite is approximately 450 nm.
Synthesis of BEA
Optimized molar composition of the gel used for the synthesis of zeolite beta (BEA) is 1.92 Na2O : 1 Al2O3 : 60 SiO2 : 444 H2O : 4.6 (TEA)2O. The mixture of sodium aluminate, sodium hydroxide and distilled water was stirred for 40 min then placed in an oven at 100 °C for 50 min to form alumina precursor solution. TEAOH as a structure directing agent was added to the cooled mixture and stirred for 15 min. Finally, Ludox HS-40 colloidal silica as a source of silica were added into the prepared precursor solution and mixed for 15 min. The resulting mixture was introduced into Teflon-lined autoclaves for crystallization. Static synthesis was carried out for 7 days at 120 °C. Product was centrifuged three times at 7500 rpm and dried overnight at 50 °C. The average particle size of zeolite beta is 1–1.5 μm.
Synthesis of Nano BEA
Molar composition of the nano beta is 0.25 Al2O3 : 25 SiO2 : 490 H2O : 9 TEAOH. Silica source was TEOS. Aluminium isopropoxide, TEAOH and distilled water were used as the other reactants. Aging was continued under static conditions for 4 h with a clear solution. Crystallization was completed within 14 days under static conditions at 100 °C in Teflon-lined autoclaves. Product was purified by using centrifugation three times at 7500 rpm . The particle size of nano beta is approximately 100 nm.
All zeolites used were calcined at 550 °C for 6 h at a rate of 1 °C/min to remove the templates and clear the pores.
Modification of BEA to BEA-Gold by Ion Exchange and Reduction
The ion exchange and reduction of zeolite beta were done by slight modification of the procedure found in the literature . The synthesized zeolite beta was calcined at 500 °C in air for 6 h before ion exchange procedure. Four hundred milligrams of calcined BEA was added to 2.33 mM Au(III)chloride solution for obtaining gold ion-exchanged BEA samples (Au(III)BEA)) according to maximum theoretical loading of 4 wt.% Au at 50 °C with stirring. After 24 h, obtained Au(III)BEA samples were washed and centrifuged for four times at 7500 rpm. Following this, the samples were dried at 50 °C under ambient air. Au(0)BEA nanoclusters were produced from gold ion reduction at 50 °C in sodium borohydride (3.4 mM) suspension and called as BEA-Gold. The reduction step was terminated when the hydrogen gas formation was finished. BEA-Gold samples were washed and centrifuged for four times at 7500 rpm and dried at 50 °C. The gold content was measured as 0.65 wt.% using ICP-OES.
Immobilization of Enzymes in Glutaraldehyde Vapour
Working solution was prepared using 10% CD and 10% BSA in 20 mM phosphate buffer (pH 7.4) containing 10% glycerol, 4% lactitol and 0.4% DEAE-Dextran. In reference solution, instead of enzyme, 20% BSA was used to hold the protein concentration constant. BSA was used both for providing amino groups for cross-linking and stabilization of enzymes. Lactitol and DEAE-Dextran were used as enzyme stabilizers. The latter and glycerol were also used to prevent the membrane from being cracked with a view to providing better adhesion to ISFET surface.
0.1 μl of working solution was deposited to one gate area of the transducer, and 0.1 μl of reference solution was deposited on the other gate area of the transducer. These transducers were exposed to saturated GA vapour for 15 min and dried at ambient air for 15 min. Finally, they were washed with buffer solution to remove the unbound protein and excess GA.
Immobilization of Enzymes onto Zeolite Layers
Drop-Coating of Zeolites onto Transducers
Different zeolite solutions with 10% concentration were prepared using 5 mM phosphate buffer (pH 7.4) and these solutions were ultrasonicated before usage to obtain a homogeneous mixture. 0.2 μl of zeolite mixture was deposited on each active zone of transducers and they were heated at 120 °C for 15 min. The obtained surfaces were washed with buffer solution to remove unbound zeolites before enzyme immobilization.
Adsorption of Enzymes onto Zeolite Layers
The working solution used for the adsorption method contained 10% CD solution in 20 mM phosphate buffer, pH 7.4 with 10% glycerol, 4% lactitol and 0.4% DEAE-Dextran whereas the reference solution contained 10% BSA instead of CD. 0.16 μl of working solution was deposited onto one gate area of the transducer, and 0.16 μl of reference solution was deposited onto the other gate area of the transducer. These transducers were dried in ambient air for 15 min.
All measurements were performed in an open stirred measuring cell filled with 5 mM phosphate buffer at pH 7.4. The substrate concentrations were varied by addition of a different volume of 500 mM stock solution to the working buffer. Each experiment was performed at least three times. Fluctuations of temperature, pH and voltage effects producing nonspecific changes in the output signal were avoided by usage of differential mode of measurements.
Characterization of Synthesized Zeolites
Physicochemical characteristics of used zeolites
S BET, (m2/g)c
S ext, (m2/g)d
Zeta potential, (mV)e
21.5 ± 0.7
21.0 ± 0.9
20.8 ± 0.8
According to Table 1, silicalite which belongs to MFI-type structure has the lowest surface area (447 m2/g) with the lowest zeta potential (−62.6 mV) and it has no aluminium inside whereas BEA-type of zeolites have similar Si/Al ratios with different particle sizes such as 1.2 μm for BEA and 100 nm for nano BEA. BEA-Gold has the highest surface area (776 m2/g) which is a result of the increase almost only in external surface area of BEA (an increase of about 20%). Additionally, nano BEA has the lowest zeta potential as −36.6 mV.
ICP results of BEA and BEA-Gold
35.80 ± 1.6
34.70 ± 0.7
1.52 ± 0.03
1.52 ± 0.02
0.45 ± 0.01
0.03 ± 0.01
0.65 ± 0.02
Contact angles of biosensor surfaces
Contact angle, Ɵ (°)
Characteristics of Zeolite-Modified ISFET Biosensors
The enzymatic reaction occurring at the dielectric gate of the transducer produces a pH increase that is proportional to creatinine concentration, which makes it detectable by the currently developed ISFET device .
Biosensor responses were compared for BEA and nano BEA samples to investigate the effect of particle size, since both zeolites had similar Si/Al ratios and identical zeolite framework (BEA). It was observed that the BEA-based one having a higher surface area (743 m2/g) with larger particle size (~1.2 μm) showed higher response. This result can also be related with its surface roughness since enzymes prefer rough surfaces rather than smooth surfaces [37–39].
In the current study, more hydrophilic surfaces gave higher sensitivities as consistent with the findings of Peng et al. They demonstrated that enzyme adsorption can be more on hydrophobic surfaces whereas hydrophilic surfaces can be more active enzymatically .
Characteristics of creatinine-based biosensors with different types of enzyme immobilization
Linear equation between response (y) and creatinine concentration (x)
Detection limit, μM
Response time, s
Regeneration time, s
y = 10.131x + 0.9594, R 2 = 0.9807
y = 20.79x − 0.3002, R 2 = 0.9934
y = 24.663x + 0.6437, R 2 = 0.9976
y = 29.591x − 0.3703, R 2 = 0.9967
y = 22.942x + 0.0394, R 2 = 0.9980
The linear dynamic range (up to 2 mM of creatinine) was same for all biosensors; however, there was a significant improvement in response time of about 60% decrease in silicalite-based biosensors and a minimal 40% decrease in regeneration time of all BEA-type of zeolite-based biosensors compared to respective values of GA-based biosensors. Additionally, the detection limit was increased twofold for all types of zeolite-based biosensors compared to GA-based biosensors.
For inter-reproducibility studies, several clean electrodes were drop-coated with zeolites then CD was adsorbed on these transducers as described in the chapter titled ‘Immobilization of enzymes onto zeolite layers’. After obtaining the response to 1 mM of creatinine in three repetitions, the enzyme and zeolites were removed from the transducers with ethanol-wetted cotton. Thereafter, the whole procedure was repeated three times and RSD values of biosensors were calculated. According to the results, the highest RSD (36.03%) belonged to the traditional method prepared with GA and the least RSD belonged to BEA-Gold (3.19%), while the intermediate values belonged to Silicalite (13.27%), BEA (10.7%) and Nano BEA (18.5%). The low RSD for zeolite-based biosensors compared to GA-based biosensors can be due to the better controlled procedure of adsorption. Additionally, low RSD of BEA-Gold can be due to the presence of gold nanoparticles, which will bind covalently the amine groups of the enzyme and actively stabilize the enzyme. This result is consistent with the study of Gole et al., where pepsin-gold colloid conjugates were prepared by just mixing under protein-friendly conditions and then demonstrated that pepsin showed significant catalytic activity. They explained the binding mechanism of the enzyme to the gold nanoparticle by covalent interactions between thiol groups in cysteine residues as well as amine groups in lysine residues of pepsin with the surface of gold. This interaction resulted in a more stabilized enzyme with its activity .
In this study, creatinine-based ISFET biosensors have been successfully produced by modifying the gate of ISFET with four different types of zeolites separately. Their responses and other biosensor characteristics such as sensitivity, detection limit, response time and regeneration time are compared with covalently cross-linked CD as a control group. All zeolite-based biosensors had higher sensitivities, lower detection limits, response and regeneration time than GA-based biosensors with good reproducibility showing that zeolites can be good alternatives for creatinine biosensor production. The incorporation of zeolite and gold nanoparticles for adsorption of CD on ISFET-based biosensor was studied for the first time. The sensitivities of the obtained biosensors were decreasing in following order: BEA-Gold > BEA > nano BEA > Silicalite > GA. The BEA-Gold-based biosensor resulted in threefold increased sensitivity compared to GA-based biosensor, which could be attributed to favourable microenvironment for CD to avoid denaturation as well as increased surface area produced by gold nanoparticles with good stability. This result showed that gold nanoparticles can be used with zeolite to improve the characteristics of ISFET-based biosensors.
The authors gratefully acknowledge the financial support of this study by the European IRSES-NANODEV project, Ministry of Education and Science of Ukraine (project DZ/45-2015) and STCU Project 6052 “Enzyme multibiosensor system for renal dysfunction diagnosis and hemodialysis control”.
BOK synthesized and characterized the zeolites with different properties. SVM performed the enzyme immobilization on the zeolite-modified ISFET and studied the analytical characteristics of obtained creatinine biosensors with BOK. BOK, SVM and BAK processed the obtained results and wrote and arranged the article. BAK planned and supervised the experiments performed by BOK and SVD. OOS and SVD proposed the idea of the development of potentiometric creatinine biosensor based on zeolite-modified electrodes. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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- Serafín V, Hernández P, Agüí L, Yáñez-Sedeño P, Pingarrón JM (2013) Electrochemical biosensor for creatinine based on the immobilization of creatininase, creatinase and sarcosine oxidase onto a ferrocene/horseradish peroxidase/gold nanoparticles/multi-walled carbon nanotubes/Teflon composite electrode. Electrochim Acta 97:175–83View ArticleGoogle Scholar
- Soldatkin AP, Montoriol J, Sant W, Martelet C, Jaffrezic-Renault N (2002) Creatinine sensitive biosensor based on ISFETs and creatinine deiminase immobilised in BSA membrane. Talanta 58:351–7View ArticleGoogle Scholar
- George SK, Dipu MT, Mehra UR, Singh P, Verma AK, Ramgaokar JS (2006) Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine. J Chromatogr B Anal Technol Biomed Life Sci 832:134–7View ArticleGoogle Scholar
- Costa ACO, da Costa JL, Tonin FG, Tavares MFM, Micke GA (2007) Development of a fast capillary electrophoresis method for determination of creatinine in urine samples. J Chromatogr A 1171:140–3View ArticleGoogle Scholar
- Killard AJ, Smyth MR (2000) Creatinine biosensors: principles and designs. Trends Biotechnol 18:433–7View ArticleGoogle Scholar
- Pundir CS, Yadav S, Kumar A (2013) Creatinine sensors. Trends Anal Chem 50:42–52View ArticleGoogle Scholar
- Walcarius A (2008) Electroanalytical applications of microporous zeolites and mesoporous (organo)silicas: recent trends. Electroanalysis 20:711–38View ArticleGoogle Scholar
- Kirdeciler SK, Soy E, Öztürk S, Kucherenko I, Soldatkin O, Dzyadevych S et al (2011) A novel urea conductometric biosensor based on zeolite immobilized urease. Talanta 85:1435–41View ArticleGoogle Scholar
- Saiapina OY, Pyeshkova VM, Soldatkin OO, Melnik VG, Kurç BA, Walcarius A et al (2011) Conductometric enzyme biosensors based on natural zeolite clinoptilolite for urea determination. Mater Sci Eng C 31:1490–7View ArticleGoogle Scholar
- Liu B, Hu R, Deng J (1997) Characterization of immobilization of an enzyme in a modified Y zeolite matrix and its application to an amperometric glucose biosensor. Anal Chem 69:2343–8View ArticleGoogle Scholar
- Phadtare S, Vinod VP, Mukhopadhyay K, Kumar A, Rao M, Chaudhari RV et al (2004) Immobilization and biocatalytic activity of fungal protease on gold nanoparticle-loaded zeolite microspheres. Biotechnol Bioeng 85:629–37View ArticleGoogle Scholar
- Kumar S, Ahlawat W, Kumar R, Dilbaghi N (2015) Graphene, carbon nanotubes, zinc oxide and gold as elite nanomaterials for fabrication of biosensors for healthcare. Biosens Bioelectron 70:498–503View ArticleGoogle Scholar
- Crumbliss, AL, Henkens, RW, Perine, SC, Tubergen, KR, Kitchell, BS, Stonehuerner J (1990). Biosensor Technology: Fundamentals and applications. Buck, R. P., Hatfield, W. E., Umana, M., Bowden EF, editor. Marcel Dekker: New YorkGoogle Scholar
- Crumbliss AL, Perine SC, Stonehuerner J, Tubergen KR, Zhao J, Henkens RW (1992) Colloidal gold as a biocompatible immobilization matrix suitable for the fabrication of enzyme electrodes by electrodeposition. Biotechnol Bioeng 40:483–90View ArticleGoogle Scholar
- Kharitonov AB, Shipway AN, Willner I (1999) An Au nanoparticle / bisbipyridinium field-effect transistor for the sensing of adrenaline. Anal Chem 71:5441–3View ArticleGoogle Scholar
- Gun J, Rizkov D, Lev O, Abouzar MH, Poghossian A, Schöning MJ (2009) Oxygen plasma-treated gold nanoparticle-based field-effect devices as transducer structures for bio-chemical sensing. Microchim Acta 164:395–404View ArticleGoogle Scholar
- Gun J, Schöning MJ, Abouzar MH, Poghossian A, Katzd E (2008) Field-effect nanoparticle-based glucose sensor on a chip: amplification effect of coimmobilized redox species. Electroanalysis 20:1748–53View ArticleGoogle Scholar
- Poghossian A, Weil MH, Bäcker M, Mayer D, Schöning MJ (2012) Field-effect devices functionalised with goldnanoparticle/ macromolecule hybrids: New opportunities for a label-free biosensing. Procedia Eng 47:273–6View ArticleGoogle Scholar
- Poghossian A, Bäcker M, Mayer D, Schöning MJ (2015) Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids. Nanoscale 7:1023–31View ArticleGoogle Scholar
- Shelyakina MK, Soldatkin OO, Arkhypova VM, Kasap BO, Akata B, Dzyadevych SV (2014) Study of zeolite influence on analytical characteristics of urea biosensor based on ion-selective field-effect transistors. Nanoscale Res Lett 9:124View ArticleGoogle Scholar
- Soldatkin OO, Shelyakina MK, Arkhypova VN, Soy E, Kirdeciler SK, Ozansoy Kasap B et al (2015) Nanosized zeolites as a perspective material for potentiometric biosensors creation. Nanoscale Res Lett 10:59View ArticleGoogle Scholar
- Soldatkin OO, Ozansoy Kasap B, Akata Kurc B, Soldatkin AP, Dzyadevych SV, El’Skaya AV (2014) Elaboration of new method of enzyme adsorption on silicalite and nano beta zeolite for amperometric biosensor creation. Biopolym Cell 30:291–8View ArticleGoogle Scholar
- Soldatkin OO, Kucherenko IS, Marchenko SV, Ozansoy Kasap B, Akata B, Soldatkin AP et al (2014) Application of enzyme/zeolite sensor for urea analysis in serum. Mater Sci Eng C 42:155–60View ArticleGoogle Scholar
- Marchenko SV, Soldatkin OO, Kasap BO, Kurc BA, Soldatkin AP, Dzyadevych SV (2016) Creatinine deiminase adsorption onto silicalite-modified pH-FET for creation of new creatinine-sensitive biosensor. Nanoscale Res Lett 11:173View ArticleGoogle Scholar
- Dzyadevych SV, Soldatkin AP, El’skaya AV, Martelet C, Jaffrezic-Renault N (2006) Enzyme biosensors based on ion-selective field-effect transistors. Anal Chim Acta 568:248–58View ArticleGoogle Scholar
- Pavluchenko AS, Kukla AL, Goltvianskyi YV, Soldatkin OO, Arkhypova VM, Dzyadevych SV, Soldatkin AP (2011) Investigation of stability of the pH-sensitive field-effect transistor characteristics. Sens Lett 9:2392View ArticleGoogle Scholar
- Prokesova P, Mintova S, Cejka JBT (2003) Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of Beta/MCM-48 phases. Microporous Mesoporous Mater 64:165–174View ArticleGoogle Scholar
- Zahmakiran M, Özkar S (2010) The preparation and characterization of gold(0) nanoclusters stabilized by zeolite framework: Highly active, selective and reusable catalyst in aerobic oxidation of benzyl alcohol. Mater Chem Phys 121:359–63View ArticleGoogle Scholar
- Yeong YF, Abdullah AZ, Ahmad AL, Bhatia S (2009) Synthesis, structure and acid characteristics of partially crystalline silicalite-1 based materials. Microporous Mesoporous Mater 123:129–39View ArticleGoogle Scholar
- Prokesova P, Mintova S, Cejka J, Bein T (2003) Preparation of nanosized micro/mesoporous composites. Mater Sci Eng C 23:1001–5View ArticleGoogle Scholar
- Narasimharao K, Hartmann M, Thiel HH, Ernst S (2006) Novel solid basic catalysts by nitridation of zeolite beta at low temperature. Microporous Mesoporous Mater 90:377–83View ArticleGoogle Scholar
- Zeng S, Ding S, Li S, Wang R, Zhang Z (2014) Controlled growth of gold nanoparticles in zeolite L via ion-exchange reactions and thermal reduction processes. Inorg Chem Commun 47:63–6View ArticleGoogle Scholar
- Jiang P, Xie S, Yao J, Pang S, Gao H (2001) The stability of self-organized 1-nonanethiol-capped gold nanoparticle monolayer. J Phys D Appl Phys 34:2255–9View ArticleGoogle Scholar
- Matsui M, Kiyozumi Y, Yamamoto T, Mizushina Y, Mizukami F, Sakaguchi K (2001) Selective adsorption of biopolymers on zeolites. Chem - A Eur J 7:1555–60View ArticleGoogle Scholar
- Sakaguchi K, Matsui M, Mizukami F (2005) Applications of zeolite inorganic composites in biotechnology: current state and perspectives. Appl Microbiol Biotechnol 67:306–11View ArticleGoogle Scholar
- Tavolaro P, Tavolaro A, Martino G (2009) Influence of zeolite PZC and pH on the immobilization of cytochrome c: a preliminary study regarding the preparation of new biomaterials. Colloids Surfaces B Biointerfaces 70:98–107View ArticleGoogle Scholar
- Rechendorff K, Hovgaard MB, Foss M, Zhdanov VP, Besenbacher F (2006) Enhancement of protein adsorption induced by surface roughness. Langmuir 22:10885–8View ArticleGoogle Scholar
- Jensen UB, Ferapontova EE, Sutherland DS (2012) Quantifying protein adsorption and function at nanostructured materials: Enzymatic activity of glucose oxidase at GLAD structured electrodes. Langmuir 28:11106–14View ArticleGoogle Scholar
- Kirdeciler SK, Ozen C, Akata B (2014) Fabrication of nano- to micron-sized patterns using zeolites: its application in BSA adsorption. Microporous Mesoporous Mater 191:59–66View ArticleGoogle Scholar
- Peng Y, Zhu-Ping H, Yong-Juan X, Peng-Cheng H, Ji-Jun T (2013) Effect of support surface chemistry on lipase adsorption and activity. J Mol Catal B: Enzym 94:69–76View ArticleGoogle Scholar
- Gole A, Dash C, Ramakrishnan V, Sainkar SR, Mandale AB, Rao M et al (2001) Pepsin - gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 17:1674–9View ArticleGoogle Scholar