Impact of Superparamagnetic Iron Oxide Nanoparticles on Vocal Fold Fibroblasts: Cell Behavior and Cellular Iron Kinetics

Table 2 Long-term cellular iron quantification

Mean pg Fe/cell	Day 5	Day 10	Day 15	Day 20	Day 25	Day 30	Day 35	Day 40
20 μg/cm²	1.42 ± 0.21***	0.79 ± 0.07***	0.32 ± 0.07***	0.10 ± 0.01***	0.09 ± 0.01	0.05 ± 0.01	0.06 ± 0.01	0.05 ± 0.01*
40 μg/cm²	1.53 ± 0.09***	0.90 ± 0.08***	0.40 ± 0.07***	0.11 ± 0.01***	0.11 ± 0.02*	0.05 ± 0.02	0.07 ± 0.01**	0.05 ± 0.01
80 μg/cm²	3.27 ± 0.033***	1.54 ± 0.35***	0.53 ± 0.08***	0.16 ± 0.06***	0.11 ± 0.01**	0.06 ± 0.01	0.07 ± 0.01*	0.05 ± 0.01
Control	0.06 ± 0.02	0.10 ± 0.03	0.08 ± 0.03	0.04 ± 0.01	0.06 ± 0.03	0.05 ± 0.02	0.04 ± 0.01	0.04 ± 0.01

Data are significantly different between values of control group and each concentration for different days, when indicated as *p ≤ 0.05, **p ≤ 0.001, and ***p ≤ 0.0001