Nanotechniques Inactivate Cancer Stem Cells

Table 1 Total number of cells in peritoneal cavity and their phenotypic parameters to day 7 after administration of total population, CD44⁺ and CD44^– EC fractions

Index		Group 1.1. Administration of total population of EC cells (3 × 10⁶ cells/animal)	Group 2.1. Administration of CD44⁺ EC cell fraction (3 × 10⁶ cells/animal)	Group 3.1. Administration of CD44^– EC cell fraction (3 × 10⁶ cells/animal)
Concentration of cells with corresponding phenotype, %	CD44^high	0.17 ± 0.03	0.32 ± 0.05^*	0.02 ± 0.002^,*
	CD44⁺CD24^–	3.78 ± 0.51	5.65 ± 0.41^*	3.81 ± 0.28^**
	CD44⁺CD24⁺	2.89 ± 0.22	4.21 ± 0.50^*	2.98 ± 0.18^**
	CD44^–CD24⁺	5.33 ± 0.64	2.00 ± 0.15^*	8.50 ± 0.59^,*
	Sca-1⁺	90.32 ± 5.33	89.05 ± 4.35	81.30 ± 6.41
	CD117⁺	7.81 ± 0.83	–	3.56 ± 0.06^*
CD44^high/CD117⁺ ratio (rel. units)		0.02	–	0.005
TN (×10⁷)		35.80 ± 2.27	826.50 ± 6.53^*	7.82 ± 0.94^,*
MF (rel.units)		116.67 ± 9.60	2755 ± 9.34*	26.07 ± 1.84^,*
TD (h)		24.47 ± 2.75	14.70 ± 1.35*	35.71 ± 2.53^,*

Notes: the indices are determined to day 7 after administration of total population and isolated CD44⁺ and CD44^– EC fractions; EC Ehrlich carcinoma, TN a total number of EC cells in peritoneal cavity (×10⁷), MF multiplicity factor, TD time doubling of total EC population in peritoneal cavity
*Differences are statistically significant if compared with group 1.1; **differences are statistically significant if compared with group 2.1 (P < 00.5)