Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene-co-acrylic Acid Composite Nanospheres

Table 3 Selectivity of DNA biosensor towards the detection of V. cholerae DNA

Real samples	(DNA) (μg/μL)	Current ± SD (μA)	Control ± SD (μA)	t test	Current (%)	Remark
Citrobacter freundii (CF-I)	1.0 × 10⁻⁴	0.250 ± 0.01	0.261 ± 0.02	1.115	2.27	×
Citrobacter freundii (CF–II)	1.0 × 10⁻⁴	0.248 ± 0.06	0.261 ± 0.02	0.267	2.26	×
Vibrio cholerae J2126-I	1.0 × 10⁻⁴	2.568 ± 0.09	0.261 ± 0.02	7.561	23.37	√
Vibrio cholerae J2126-II	1.0 × 10⁻⁴	2.579 ± 0.10	0.261 ± 0.02	7.983	23.47	√
Vibrio cholerae J3324-I	1.0 × 10⁻⁴	10.117 ± 0.20	0.261 ± 0.02	15.630	92.06	√
Vibrio cholerae J3324-II	1.0 × 10⁻⁴	10.990 ± 0.24	0.261 ± 0.02	18.299	100.00	√
Vibrio cholerae J3330-I	1.0 × 10⁻⁴	2.800 ± 0.43	0.261 ± 0.02	7.926	25.48	√
Vibrio cholerae J3330-II	1.0 × 10⁻⁴	1.864 ± 0.34	0.261 ± 0.02	5.128	16.96	√
Vibrio cholerae CDHI5294-II	1.0 × 10⁻⁴	3.705 ± 0.41	0.261 ± 0.02	8.124	33.71	√
Vibrio cholerae UVC1324	1.0 × 10⁻⁴	10.904 ± 0.25	0.261 ± 0.02	18.115	99.00	√

√-DPV peak current is significantly higher than the control’s response. × − DPV peak current is significantly lower than the control’s response at 95% confidence level and 4 degrees of freedom