Fig. 5
From: Functionalized Nano-adsorbent for Affinity Separation of Proteins
SDS-PAGE analysis of the purified recombinant GPX3, OST1, and ABI2 proteins. Lanes 1, 4, and 7, E. coli lysate; lanes 2, 5, and 8, the proteins eluted from commercial Glutathione Sepharose 4B (GE Healthcare, USA); lanes 3, 6, and 9, the proteins eluted from SnO2/SiO2-GSH NSs; lane 10, the marker; lanes 11 and 13, GPX3 obtained after the GST tag is cut off from Glutathione Sepharose 4B bound GST-GPX3 and SnO2/SiO2-GSH NSs bound GST-tagged GPX3; lanes 12 and 14, GST tag eluted from Glutathione Sepharose 4B and SnO2/SiO2-GSH NSs