- Nano Express
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Development and Characterization of Sr-Containing Glass-Ceramic Composites Based on Biogenic Hydroxyapatite
© The Author(s). 2018
Received: 30 December 2017
Accepted: 20 April 2018
Published: 16 May 2018
Composite materials based on hydroxyapatite are widely used for bone tissue engineering. There is evidence of a positive effect of the presence of strontium in osteoplastic materials in the case of a Ca/Sr certain ratio. To examine the effect of the addition of Sr2+, a study was made by introducing it into the material composition based on biogenic hydroxyapatite and sodium borosilicate glass (50/50% wt.). The strontium was introduced into the composition in an amount of 1% wt. Composite materials were obtained at final sintering temperatures of 780 °C and a sintering time of 1 h. The effect of additions of glass phase and strontium affect changes in the crystal lattice of biogenic hydroxyapatite was investigated with the help of X-ray phase analysis, IR spectroscopy. Also the behavior of composites in vitro in physiological solution was studied.
Hydroxyapatite (HA) and other bioactive calcium phosphate materials, including bioactive glass, can be used in tissue engineering to replace bone tissue. Natural biogenic hydroxyapatite (BHA) is nano-structural material, nano-, and microstructure of which is analogous to mineral component of bone tissue.
Earlier production results were received, and various types of composites on the base of biogenic and synthetic hydroxyapatite and glass phase were studied [1–5]. It is known that, in the chemical composition of natural biogenic hydroxyapatite (BHA), there are small amounts of other non-organic compounds such as Ca4O(PO4)2, NaCaPO4, Ca3(PO4)2, CaO, and MgO, as well as traces of Al3+, Fe3+, Mg2+, Sr2+, K+, Si4+, Na+, Cu2+, Zn2+, and CO32−[6–9]. Therefore, composites of BHA/glass with the use of various bioactive additives that can contribute to the processes of osteogenesis and formation of new bone tissue were also studied and obtained. These composites were alloyed by ion additives Fe2+/Fe3+ and Cu2+ [10, 11], Ce2+ , Si4+ , La3+ , and other.
A modern problem in the field of diseases of bone pathology is the treatment of osteoporosis that is the rarefaction of the density of the patient’s bone tissue as a result of hormonal or other causes. That is why it is quite relevant to develop new bioactive materials that can stimulate the formation of new cells of bone tissue and improve its structure. From this point of view, the introduction of strontium ions into the composition of calcium phosphate materials can improve their bioactive properties [15–17].
Development of instruments used for bone tissue regeneration in vivo is focused on cellular models and processes of differentiation.
The authors [15, 18] point at the role of Sr2+in maintaining bone regeneration during the process of skeletal restoration as a whole, and more specifically, in application of cellular therapy. For example, strontium ranelatere presents itself an interesting compound that stimulates bone formation and inhibits bone resorption. In the estimation of effect of the wide range of strontium (Sr2+) concentration in vitro, the authors established that Sr2+ promoted a proliferation of PA20-h5 cells as well as HA formation in the course of osteoinduction in vitro. Data mentioned above point to the role of Sr2+ in maintaining bone regeneration during the process of skeletal restoration as a whole, and more specifically, in application of cellular therapy.
The authors of this work  have received stable suspensions of nanopowders of strontium-containing hydroxyapatite (Sr-HA) by method of precipitation from aqueous solution (strontium content in the amount from 0 to 100 mol%). Studies of materials obtained point to the complete solubility of strontium in hydroxyapatite lattice in isomorphic replacement of Ca2+ for Sr2+. Addition of strontium is responsible for the increase of ratio c/a in the triclinic elementary cell. A significant change in the form and size of nanopowders is observed, in which connection a prevailing growth is in the direction along c axis manifested in case of higher strontium content.
Data of suspensions biocompatibility studies, from the point of view of viability of cells, apoptosis, proliferation, and morphology with the use of cell line of osteosarcoma SAOS-2 point to the increased proliferation of cells for HA nanoparticles containing greater number of Sr2 +, in so doing, it has, practically, no effect on morphology of cells.
In the work , multifunctional porous granules were developed as fillers of bone tissue and carriers of medicinal agents. Powders of strontium and magnesium-replaced material HA/TCP with compositions close to the mineral part of human bone [(Ca + Sr + Mg)/P = 1,62], have been obtained by precipitation, then subjected to heat treatment and deagglomeration. Drying and sintering permitted to receive porous granules that were saturated with antibiotic (levofloxacin) solution, frozen, and then lyophilized. The estimation of medicinal agent release and osteocompatibility of granules has shown that Sr-alloyed granules have demonstrated the highest levels of proliferation and effectiveness in osteoblastic maturation.
There are some data about the influence of strontium additive on the behavior of bioactive glass 1393 nBG particles in vitro, from the point of view of physic-chemical reactions occurring on their surface in SBF . In comparison with non-alloyed glass 1393 nBG, it was found out that apatite formation is slowed down in Sr introduction that, probably, is the result of inhibiting action of Sr ions on HA crystallization. In addition, it was revealed that crystallinity of precipitated calcium phosphate was lower in the case with Sr-alloyed glass 1393 nBG compared to a pure standard material 1393 nBG. The authors of the abovementioned work also point out that the ability to form HA bioactive glasses gives the notion about their surface reactivity that has relation to the application of the nano-scaled particles of biologically active glass in bone regeneration.
Studies carried out in vivo on rats by the authors of work  have shown positive impact of strontium-containing macroporous bioactive glass (Sr-MBG) on the process of bone formation as well as on the decrease of bone resorption in rats with osteoporosis (osteoporotic rats). Investigation has demonstrated that the use of (Sr-MBG) leads to the increase of bone formation (46.67%) in comparison with non-alloyed glass MBG (39.33%) and control group (17.50%).
The aim of the work is preparation and investigation of bioactive materials based on biogenic hydroxyapatite/glass composite with SrО-additive for tissue engineering.
Bioactive glass-ceramic composites based on nanostructured biogenic hydroxyapatite (BHA) with addition of sodium borosilicate glass (mass%: 46 SiO2; 28 B2O3; 26 Na2O) were prepared as described in . To obtain Sr-containing glass-ceramic composites samples were used powders of bioactive glass-ceramic composites based on nanostructured biogenic hydroxyapatite (BHA) and sodium borosilicate glass with the relation 50/50% wt and sintered at the temperature 1100 °C (start sintering), then were crushed. Obtained powders of the composites were mixed with powder of strontium oxide (1 wt.%). The samples of BHA/glass and BHA/glass-Sr composites of 2.5 g and 11 mm diameter were formed and sintered at the temperatures 780 °C.
The phase composition and structure of the obtained samples were studied by X-ray diffraction, IR spectroscopy, and SEM.
For X-ray analysis, the X-ray diffractometer DRON-3M was used equipped with additional computer system of scanning, X-ray tube with copper anode and nickel filter. Thus, diffraction images were obtained with the help of Cu–Kα radiation with a medium wavelength λ = 1,54178 Ǻ3.
In addition, the materials were studied by infrared (IR) spectroscopy using a spectrophotometer FSM 1202 (TOV Infraspectr, Russia) in the wavenumber range 4000–400 cm− 1.
The structure of the composites was studied by scanning electron microscopy (SEM) using a REM-106I (VAT SELMI, Ukraine).
ρpykn is the pycnometric density of compact material, g/cm3.
For BHA ρpykn = 3.00 g/cm3.
m 1 is the saturated sample weight in air, g;
m 2 is the saturated sample weight in water, g;
ρ w is the density of water, g/cm3;
ρliq is the density of the saturating liquid (ethylene), g/cm3.
In Vitro Bioactivity Testing
Investigation of solubility in vitro of porous samples was carried out in an isotonic saline solution (0.9% NaCl) at a solid/liquid ratio of 1:30 after 2, 5, and 7 days exposition in a thermostat at 36.5 ± 0.5 °C followed by determination of mass loss on an analytic balance “OHAUS Pioneer PA214C” (OHAUS Corporation, China) with an accuracy of 0.0001 g.
Results and Discussion
Initial powder BGA demonstrates a diffraction image corresponding to PDF file 72-1243 (Tables JCPDS) (Fig. 1). Indicated standard diffraction image belongs to crystals with hexagonal primitive crystal lattice having periods of а = 9432 and с = 6881 Å. Calculation of periods is performed with the use of peaks (002), (211), (300), (222), and (213), lying in the range of 2θ = 24°–52°. Accordingly, the volume of elementary crystal cell of such crystals is equal to 530,496 Ǻ3, that insignificantly differs from that indicated in the standard file value – 530.14 Ǻ3.
At the expense of presence of amorphous phase in the composite BHA/glass, a process of liquid-phase sintering occurs. As the result of active interaction of components of glass phase and ВНА, a formation of new crystal phases takes place. By the results of XPA were determined basic crystal phases of BHA/glass composite (Fig. 2). It was established that obtained composites are heterogeneous material. The following phases are presented with the most intensive peaks: Ca10(PO4)6(OH)2, Na2Ca3Si3O10, Ca2SiO4, Na4SiO4, Na2BO2.
The volume of crystal cell depends on its perfection, i.e., appropriate filling with ions forming a part of hydroxyapatite. Structural features of elementary HA cell are studied sufficiently deep [9, 22, 23]. Using existing data, we can state that a plane (004) has in its composition atoms of phosphorus, oxygen, and calcium, while a plane (211) is formed with atoms of oxygen and (202)– with ions СаII2+. The main amount of oxygen is in the composition of tetrahedrons РО43−. Since the relative intensity depends on atomic factor of dissipation (other factors are identical), a relative intensity of peak (202)–I(202)/I(211) can indirectly testify to the changes in the number of vacancies in ions Са2+ or their absence. It should be noted that atomic scattering factor for Cu-Kα radiation at sinθ/λ=0.5 for Са2+ ions is equal to 8.1, and for Sr2+ ions – 19.6 . Replacement of ions Са2+ for Sr2+ ions increases periods а and с of crystal lattice in hydroxyapatite . So, if the above replacement leads to a formula of solid solution Ca10−xSr x (PO4)6(OH)2, where х = 1, then a volume of the elementary crystal cell exceeds the value ~ 540 Ǻ3.
Introduction of strontium oxide in the amount of 1% by mass into the composition of BHA/glass does not substantially change a phase composition of composites; this can be seen in comparison of diffraction images, located in one diagram without any change of intensity (Figs. 3 and 4).
However, there are differences in the position of centers of peaks BHA/glass and BHA/glass-Sr, as well as the decrease in the intensity of peaks and their shift to the side of large angles in BHA/glass-Sr (Fig. 4). Probably, that strontium introduction into BHA/glass leads to the decrease of the number of crystal phases compared to a non-alloyed composite, as well as contributes to the formation of crystalline structure of composite at the expense of transition of crystal phases to amorphous (Fig. 3). In Fig. 4, main peaks of ВНА are given that are expanded in the angular range 2θ and designated with vertical lines.
Parameters of HA crystal structure in BHA/glass composites
As it is known , hydroxyapatite spectrum is characterized with two intensive groups of bands about 1040 and 570 cm− 1.
In our case, strontium was introduced into the prepared composite material that, probably, involved a change in the composition of glass phase with weakening in the structure of silicon-oxygen framework. As the result of the above processes, it decreases a viscosity that is the basic factor determining the diffusion processes in sintering  and causes foaming of crystalline glass composite. And as the result, probably, it leads to the increase of interaction on the surface of ВНА/glass, i.e., ion exchange between ВНА and glass phase is increased. Probably, it leads to a partial replacement of calcium ions for strontium ions in the structure of ВНА, with subsequent transition of calcium ions into glass phase with formation of new crystal phases that leads to the subsequent changes not only in crystal lattice, but in the structure of the whole composite.
As a whole, it is evident that the microstructure of the internal part of samples is polyporous, in other words, it is characterized by the presence of multi-sized pores, which is promising in view of the achievement of high resorption properties of bioceramics under direct contact with human organism media.
Since the content of glass phase in both types of composites is practically identical, therefore, the influence of its quantity on the dissolution will be also analogous. Thus, the results obtained may be caused by significantly higher porosity of BHA/glass-Sr composites, as well as by greater share of open porosity compared to BHA/glass composites.
Obtained composites can be applied in medicine to replace some defective areas of patients’ bone tissue. Availability of the wide range of pore sizes in the obtained materials enables to use them in various regions of human skeleton.
New, strontium-doped composite materials are obtained on the base of biogenic hydroxyapatite and glass phase. XPA showed that obtained composites are heterogeneous material. Content of glass phase and strontium as alloyed additive in the composite material of BHA/glass has influence on the change of parameters of BHA elementary cell, namely, leads to the decrease of crystal lattice of hydroxyapatite. It was also established that BHA/glass-Sr composite possesses a higher porosity as well as higher rate of dissolution in physiologic solution that makes the composites obtained as perspective materials to be used in medicine for replacement of defective areas of patients’ bone tissue.
The authors would like to express their sincere thanks to Dr. L. Ivanchenko for her assistance in planning the experiment.
This work was supported by the International research and practice conference “Nanotechnology andNanomaterials” NANO-2017 (August 23–26, 2017, Chernivtsi, Ukraine).
Availability of Data and Materials
The datasets supporting the conclusions of this article are included within the article and its additional files (figures).
OK is responsible for the preparation of samples, porosity measurement, in vitro bioactivity testing, analysis and interpretation of IR, XRF, and microstructure results. NP is responsible for the analysis and interpretation of results. OlB and OO are responsible for the X-ray diffraction analysis. TT is responsible for the IR-spectroscopy analysis. MG is responsible for the scanning electron microscopy. All authors read and approved the final manuscript.
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