Skip to main content
Fig. 8 | Nanoscale Research Letters

Fig. 8

From: Evaluating the antimicrobial, apoptotic, and cancer cell gene delivery properties of protein-capped gold nanoparticles synthesized from the edible mycorrhizal fungus Tricholoma crassum

Fig. 8

Assay of apoptotic properties of the AuNPs. a Comet assay and fluorescent microscopic images of nuclei of tobacco leaves treated with AuNPs for A 0 min. B 15 min, C 20 min, and D 30 min (bar = 5 μm). In the control sets (0 min incubation), most of the DNA is located in the head of the comet while cells subjected to longer treatment show increasing DNA damage and longer comet tails. b Mean of % tail DNA ± SE after different periods of incubation. Data represents means ± SE of three replicates. Different letters indicate statistically significant differences among the samples (P < 0.05, Tukey’s HSD test). c Assay of threshold dosage of AuNPs for apoptosis showing images of nuclei of tomato leaf cells treated with A 0% (control), B 5%, C 10%, D 15%, E 20% of AuNP suspension for 24 h (bar = 10 μm). d Mean % tail DNA ± SE after 24 h treatment with different concentration of AuNPs. Data represents means ± SE of three replicates. Different letters indicate statistically significant differences among the samples (P < 0.05, Tukey’s HSD test). Dosage below 10% show negligible DNA damage while higher than 20% show start of DNA damage

Back to article page