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Fig. 4 | Nanoscale Research Letters

Fig. 4

From: Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression

Fig. 4

AFM force measurements with integrin β1-functionalized AFM tip on living hADSc. a Schematic representation of the strategy used for immobilization of integrin β1 onto an AFM tip. b Schematic representation of the single-molecule force measured between integrin β1-functionalized AFM tips and living hADSc. c, d Representative force curves obtained with integrin β1-modified AFM tips on hADSc, and e after the system was blocked with the integrin β1 monoclonal antibody solution. f The binding probability of integrin β1-functionalized tips on hADSc before and after blocking by integrin β1 antibody on day 0. g The binding probability of CD29-functionalized tips on hADSc after blocking by integrin β1 antibody at 0, 6, and 12 days. ***p < 0.001, n.s.. no significant difference

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