Preparation of Glycyrrhetinic Acid Liposomes Using Lyophilization Monophase Solution Method: Preformulation, Optimization, and In Vitro Evaluation

Table 1 Single-factor experiments

Factor		MD (nm)^a	EE (%)^b	Score	Other condition
Quantity of SPC (mg)	240	221.8	45.63	− 0.052	Quantity of cholesterol 60 mg; volume percentage of TBA 50%
	360	224.6	55.86	0.019
	480	230.4	64.47	0.073
	600	245.8	66.49	0.061
	720	284.6	64.77	− 0.020
Quantity of cholesterol (mg)	60	225.3	56.35	0.015	Quantity of SPC 480 mg; volume percentage of TBA 50%
	120	230.4	64.47	0.069
	180	238.6	63.13	0.043
	240	235.8	60.57	0.029
	300	267.2	55.62	− 0.069
Volume percentage of TBA (%)	40%	230.4	64.47	0.056	Quantity of SPC 480 mg; quantity of cholesterol 120 mg
	50%	226.7	65.13	0.068
	60%	219.4	61.83	0.056
	70%	224.2	62.72	0.054