Table 4 Addition of trehalose after homogenization
Formula code | (Lipid:trehalose) ratio | Testing condition | PS (nm) | PDI | % EE |
|---|---|---|---|---|---|
VER-9 | 1:0 | Before lyophilization | 178.34 ± 4.85 | 0.534 ± 0.0002 | 97.98 ± 0.96 |
After lyophilization | 372.78 ± 10.81 | 0.733 ± 0.048 | 97.61 ± 0.51 | ||
1:1 | After lyophilization | 212.82 ± 3.83 | 0.544 ± 0.044 | 67.47 ± 1.61 | |
1:2 | 329.45 ± 12.10 | 0.995 ± 0.007 | 40.68 ± 7.32 | ||
1:4 | 227.93 ± 3.64 | 0.634 ± 0.812 | 22.19 ± 3.25 | ||
1:6 | 317.94 ± 65.10 | 0.812 ± 0.052 | 9.47 ± 2.93 | ||
1:8 | 260.67 ± 16.35 | 0.611 ± 0.033 | 8.37 ± 2.02 | ||
VER-11 | 1:0 | Before lyophilization | 166.34 ± 3.84 | 0.465 ± 0.012 | 99.09 ± 0.29 |
After lyophilization | 1544.18 ± 2064 | 1 ± 0.00 | 98.27 ± 0.34 | ||
1:1 | After lyophilization | 171.59 ± 3.69 | 0.558 ± 0.033 | 70.28 ± 0.87 | |
1:2 | 210.97 ± 48.38 | 0.610 ± 0.041 | 44.88 ± 3.55 | ||
1:4 | 264.33 ± 13.52 | 0.748 ± 0.022 | 23.81 ± 3.17 | ||
1:6 | 231.9 ± 17.64 | 0.549 ± 0.025 | 9.06 ± 2.75 | ||
1:8 | 260.33 ± 16.30 | 0.506 ± 0.078 | 7.96 ± 2.56 | ||
VER-13 | 1:0 | Before lyophilization | 165.5 ± 4.34 | 0.524 ± 0.174 | 93.90 ± 0.77 |
After lyophilization | 1129.41 ± 657.64 | 1 ± 0.00 | 92.77 ± 1.37 | ||
1:1 | After lyophilization | 261.11 ± 13.35 | 0.608 ± 0.025 | 71.53 ± 2.85 | |
1:2 | 188.66 ± 9.19 | 0.662 ± 0.044 | 52.81 ± 2.50 | ||
1:4 | 256.45 ± 22.60 | 1 ± 0.00 | 25.19 ± 3.90 | ||
1:6 | 272.61 ± 34.13 | 0.622 ± 0.025 | 10.64 ± 0.82 | ||
1:8 | 238.02 ± 12.73 | 0.824 ± 0.030 | 8.43 ± 3.08 | ||
VER-15 | 1:0 | Before lyophilization | 174.52 ± 2.97 | 0.497 ± 0.012 | 95.87 ± 0.25 |
After lyophilization | 1175.55 ± 1016.11 | 1 ± 0.00 | 94.90 ± 1.26 | ||
1:1 | After lyophilization | 232.77 ± 4.55 | 0.497 ± 0.069 | 74.95 ± 3.18 | |
1:2 | 300.91 ± 21.33 | 0.737 ± 0.036 | 46.04 ± 9.01 | ||
1:4 | 270.42 ± 19.17 | 0.659 ± 0.094 | 27.28 ± 4.32 | ||
1:6 | 289.23 ± 42.33 | 0.824 ± 0.030 | 9.39 ± 2.48 | ||
1:8 | 208.55 ± 10.87 | 0.738 ± 0.022 | 7.78 ± 3.00 |