Fig. 6From: Novel Delivery of Mitoxantrone with Hydrophobically Modified Pullulan Nanoparticles to Inhibit Bladder Cancer Cell and the Effect of Nano-drug Size on Inhibition EfficiencyApoptosis of mitoxantrone and nanodrugs at 24 h on MB49 bladder cancer cells(a DMSO, b mitoxantrone, c CHP-3, d CHP-2, e CHP-1): A. Annexin V-FITC/PI double staining was detected by fluorescence microscopy, early apoptotic cells showed Annexin V-FITC–positive staining (green), necrotic cells were PI-positive (red), and late apoptotic cells showed positive double staining (yellow). B. Apoptotic rate was determined by FCM. Live cell (Q3), early apoptotic rate (Q4), late apoptotic rate (Q2), necrotic cells (Q1).The greater the cell staining, the higher the apoptotic rateBack to article page